RAD34_YEAST
ID RAD34_YEAST Reviewed; 692 AA.
AC Q06665; D6VSU3;
DT 17-OCT-2006, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1996, sequence version 1.
DT 03-AUG-2022, entry version 134.
DE RecName: Full=DNA repair protein RAD34;
GN Name=RAD34; OrderedLocusNames=YDR314C;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=9169867;
RA Jacq C., Alt-Moerbe J., Andre B., Arnold W., Bahr A., Ballesta J.P.G.,
RA Bargues M., Baron L., Becker A., Biteau N., Bloecker H., Blugeon C.,
RA Boskovic J., Brandt P., Brueckner M., Buitrago M.J., Coster F.,
RA Delaveau T., del Rey F., Dujon B., Eide L.G., Garcia-Cantalejo J.M.,
RA Goffeau A., Gomez-Peris A., Granotier C., Hanemann V., Hankeln T.,
RA Hoheisel J.D., Jaeger W., Jimenez A., Jonniaux J.-L., Kraemer C.,
RA Kuester H., Laamanen P., Legros Y., Louis E.J., Moeller-Rieker S.,
RA Monnet A., Moro M., Mueller-Auer S., Nussbaumer B., Paricio N., Paulin L.,
RA Perea J., Perez-Alonso M., Perez-Ortin J.E., Pohl T.M., Prydz H.,
RA Purnelle B., Rasmussen S.W., Remacha M.A., Revuelta J.L., Rieger M.,
RA Salom D., Saluz H.P., Saiz J.E., Saren A.-M., Schaefer M., Scharfe M.,
RA Schmidt E.R., Schneider C., Scholler P., Schwarz S., Soler-Mira A.,
RA Urrestarazu L.A., Verhasselt P., Vissers S., Voet M., Volckaert G.,
RA Wagner G., Wambutt R., Wedler E., Wedler H., Woelfl S., Harris D.E.,
RA Bowman S., Brown D., Churcher C.M., Connor R., Dedman K., Gentles S.,
RA Hamlin N., Hunt S., Jones L., McDonald S., Murphy L.D., Niblett D.,
RA Odell C., Oliver K., Rajandream M.A., Richards C., Shore L., Walsh S.V.,
RA Barrell B.G., Dietrich F.S., Mulligan J.T., Allen E., Araujo R., Aviles E.,
RA Berno A., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M.,
RA Hunicke-Smith S., Hyman R.W., Komp C., Lashkari D., Lew H., Lin D.,
RA Mosedale D., Nakahara K., Namath A., Oefner P., Oh C., Petel F.X.,
RA Roberts D., Schramm S., Schroeder M., Shogren T., Shroff N., Winant A.,
RA Yelton M.A., Botstein D., Davis R.W., Johnston M., Andrews S., Brinkman R.,
RA Cooper J., Ding H., Du Z., Favello A., Fulton L., Gattung S., Greco T.,
RA Hallsworth K., Hawkins J., Hillier L.W., Jier M., Johnson D., Johnston L.,
RA Kirsten J., Kucaba T., Langston Y., Latreille P., Le T., Mardis E.,
RA Menezes S., Miller N., Nhan M., Pauley A., Peluso D., Rifkin L., Riles L.,
RA Taich A., Trevaskis E., Vignati D., Wilcox L., Wohldman P., Vaudin M.,
RA Wilson R., Waterston R., Albermann K., Hani J., Heumann K., Kleine K.,
RA Mewes H.-W., Zollner A., Zaccaria P.;
RT "The nucleotide sequence of Saccharomyces cerevisiae chromosome IV.";
RL Nature 387:75-78(1997).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [3]
RP SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
RX PubMed=14562095; DOI=10.1038/nature02026;
RA Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W.,
RA Weissman J.S., O'Shea E.K.;
RT "Global analysis of protein localization in budding yeast.";
RL Nature 425:686-691(2003).
RN [4]
RP LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
RX PubMed=14562106; DOI=10.1038/nature02046;
RA Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N.,
RA O'Shea E.K., Weissman J.S.;
RT "Global analysis of protein expression in yeast.";
RL Nature 425:737-741(2003).
RN [5]
RP FUNCTION.
RX PubMed=15916602; DOI=10.1111/j.1365-2958.2005.04607.x;
RA den Dulk B., Brandsma J.A., Brouwer J.;
RT "The Rad4 homologue YDR314C is essential for strand-specific repair of RNA
RT polymerase I-transcribed rDNA in Saccharomyces cerevisiae.";
RL Mol. Microbiol. 56:1518-1526(2005).
CC -!- FUNCTION: Involved in nucleotide excision repair (NER) of damaged
CC ribosomal DNA (rDNA). Required for the repair of the RNA polymerase I-
CC transcribed strand of rDNA. {ECO:0000269|PubMed:15916602}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:14562095}.
CC -!- MISCELLANEOUS: Present with 78 molecules/cell in log phase SD medium.
CC {ECO:0000269|PubMed:14562106}.
CC -!- SIMILARITY: Belongs to the XPC family. {ECO:0000305}.
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DR EMBL; U28374; AAB64750.1; -; Genomic_DNA.
DR EMBL; BK006938; DAA12153.1; -; Genomic_DNA.
DR PIR; S61200; S61200.
DR RefSeq; NP_010600.1; NM_001180622.1.
DR AlphaFoldDB; Q06665; -.
DR BioGRID; 32367; 61.
DR DIP; DIP-4550N; -.
DR IntAct; Q06665; 3.
DR MINT; Q06665; -.
DR STRING; 4932.YDR314C; -.
DR PaxDb; Q06665; -.
DR PRIDE; Q06665; -.
DR EnsemblFungi; YDR314C_mRNA; YDR314C; YDR314C.
DR GeneID; 851909; -.
DR KEGG; sce:YDR314C; -.
DR SGD; S000002722; RAD34.
DR VEuPathDB; FungiDB:YDR314C; -.
DR eggNOG; KOG2179; Eukaryota.
DR HOGENOM; CLU_028212_0_0_1; -.
DR InParanoid; Q06665; -.
DR OMA; CFALPLH; -.
DR BioCyc; YEAST:G3O-29873-MON; -.
DR Reactome; R-SCE-3108214; SUMOylation of DNA damage response and repair proteins.
DR Reactome; R-SCE-5696395; Formation of Incision Complex in GG-NER.
DR PRO; PR:Q06665; -.
DR Proteomes; UP000002311; Chromosome IV.
DR RNAct; Q06665; protein.
DR GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR GO; GO:0000111; C:nucleotide-excision repair factor 2 complex; IBA:GO_Central.
DR GO; GO:0005634; C:nucleus; HDA:SGD.
DR GO; GO:0071942; C:XPC complex; IBA:GO_Central.
DR GO; GO:0003684; F:damaged DNA binding; IBA:GO_Central.
DR GO; GO:0003697; F:single-stranded DNA binding; IBA:GO_Central.
DR GO; GO:0006298; P:mismatch repair; IBA:GO_Central.
DR GO; GO:0006289; P:nucleotide-excision repair; IMP:SGD.
DR Gene3D; 3.30.70.2460; -; 1.
DR Gene3D; 3.90.260.10; -; 1.
DR InterPro; IPR018327; BHD_2.
DR InterPro; IPR004583; DNA_repair_Rad4.
DR InterPro; IPR018326; Rad4_beta-hairpin_dom1.
DR InterPro; IPR018328; Rad4_beta-hairpin_dom3.
DR InterPro; IPR042488; Rad4_BHD3_sf.
DR InterPro; IPR036985; Transglutaminase-like_sf.
DR PANTHER; PTHR12135; PTHR12135; 2.
DR Pfam; PF10403; BHD_1; 1.
DR Pfam; PF10405; BHD_3; 1.
DR SMART; SM01030; BHD_1; 1.
DR SMART; SM01031; BHD_2; 1.
DR SMART; SM01032; BHD_3; 1.
PE 1: Evidence at protein level;
KW DNA damage; DNA repair; Nucleus; Reference proteome.
FT CHAIN 1..692
FT /note="DNA repair protein RAD34"
FT /id="PRO_0000253814"
FT REGION 1..38
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1..15
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 16..30
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 692 AA; 82043 MW; EF63BF1F002E7F2D CRC64;
MAKRLLESSQ NDQANRKNSK IEKKEVSFYE EEETDDSFDS FYQDEEDDLS DIDWEEVSLD
GSLTVTFGNI RRDREKVSKY KRKHNKKAFN YQRLKYGLHL IMIPFMLFLL KSRMKWIDDE
RLNRRLRRSV PKLIGKKFKD WDVRDPAFKM DSLRTLLLGL VLWFRSNYKM NSNGIRQNFN
RLQYLIKYAD NQNENSISES TYKKVLENQQ EFYGNRPLIN HGVEDIRKMA KRKMANRDIL
TLFFFIILEN VLPGPKKLYL CFALPLHDYD IRCNKVKWQI EHGIGKVPNR FDSDLIQPYF
WIELEVPTLS DGELYIIDPI AHLGEREMVL KTREDQFVPT YQPSVDMKYN LNQKFHYVVR
INHAEKVLQD VSPRYVPNVC YRYFELSESS PILKSKHYTS YQYLSKWLKV LNKKKASVHH
YAIMKKIALT NFTLPKSVTE IKRTDNFVIP SLLKSNEVLK ACAKQAATFT KGDNSQEPIF
WRRDVIQLKS KQHWAILGRS ILPNAQPLKR KKYLPMRERM VRNLDKYVIK ELFSYEQTMK
SPKYPSTYCD HLGQEHVITD LSHYKNKFGN IEIYSKETKP DGFELIPLSK EVDIKCLIKE
YNKGKRKMQK IKYLDVVSGF DFKQKKGHAI PKIESILVKE TDYKAVQLLK QQTKVLLGLS
FWDILLRKLR VNDRLNAEYG NVGNNEETLD DH
