RAD54_DROGR
ID RAD54_DROGR Reviewed; 786 AA.
AC B4JCS7;
DT 23-MAR-2010, integrated into UniProtKB/Swiss-Prot.
DT 23-SEP-2008, sequence version 1.
DT 03-AUG-2022, entry version 68.
DE RecName: Full=DNA repair and recombination protein RAD54-like {ECO:0000250|UniProtKB:O76460};
DE EC=3.6.4.-;
DE AltName: Full=Protein okra {ECO:0000250|UniProtKB:O76460};
GN Name=okr {ECO:0000250|UniProtKB:O76460}; ORFNames=GH10642;
OS Drosophila grimshawi (Hawaiian fruit fly) (Idiomyia grimshawi).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Hawaiian Drosophila.
OX NCBI_TaxID=7222;
RN [1] {ECO:0000312|EMBL:EDW03166.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Tucson 15287-2541.00 {ECO:0000312|EMBL:EDW03166.1};
RX PubMed=17994087; DOI=10.1038/nature06341;
RG Drosophila 12 genomes consortium;
RT "Evolution of genes and genomes on the Drosophila phylogeny.";
RL Nature 450:203-218(2007).
CC -!- FUNCTION: Involved in mitotic DNA repair and meiotic recombination.
CC Functions in the recombinational DNA repair pathway. Essential for
CC interhomolog gene conversion (GC), but may have a less important role
CC in intersister GC than spn-A/Rad51. In the presence of DNA, spn-A/Rad51
CC enhances the ATPase activity of okr/Rad54 (By similarity).
CC {ECO:0000250|UniProtKB:O76460}.
CC -!- SUBUNIT: Interacts (via N-terminus) with spn-A/Rad51. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250|UniProtKB:O76460}.
CC -!- SIMILARITY: Belongs to the SNF2/RAD54 helicase family. {ECO:0000255}.
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DR EMBL; CH916368; EDW03166.1; -; Genomic_DNA.
DR RefSeq; XP_001988299.1; XM_001988263.1.
DR AlphaFoldDB; B4JCS7; -.
DR SMR; B4JCS7; -.
DR STRING; 7222.FBpp0144548; -.
DR EnsemblMetazoa; FBtr0146056; FBpp0144548; FBgn0118123.
DR GeneID; 6562163; -.
DR KEGG; dgr:6562163; -.
DR eggNOG; KOG0390; Eukaryota.
DR HOGENOM; CLU_000315_10_5_1; -.
DR InParanoid; B4JCS7; -.
DR OMA; YTEHERM; -.
DR OrthoDB; 93727at2759; -.
DR PhylomeDB; B4JCS7; -.
DR ChiTaRS; okr; fly.
DR Proteomes; UP000001070; Unassembled WGS sequence.
DR GO; GO:0005634; C:nucleus; ISS:UniProtKB.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0140658; F:ATP-dependent chromatin remodeler activity; IEA:InterPro.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0004386; F:helicase activity; IEA:UniProtKB-KW.
DR GO; GO:0016817; F:hydrolase activity, acting on acid anhydrides; IEA:InterPro.
DR GO; GO:0051301; P:cell division; IEA:UniProtKB-KW.
DR GO; GO:0006338; P:chromatin remodeling; ISS:UniProtKB.
DR GO; GO:0043150; P:DNA synthesis involved in double-strand break repair via homologous recombination; ISS:UniProtKB.
DR GO; GO:0000724; P:double-strand break repair via homologous recombination; ISS:UniProtKB.
DR GO; GO:0045003; P:double-strand break repair via synthesis-dependent strand annealing; IEA:EnsemblMetazoa.
DR GO; GO:0000711; P:meiotic DNA repair synthesis; ISS:UniProtKB.
DR GO; GO:0030716; P:oocyte fate determination; IEA:EnsemblMetazoa.
DR GO; GO:0048477; P:oogenesis; IEA:EnsemblMetazoa.
DR GO; GO:0007131; P:reciprocal meiotic recombination; IEA:EnsemblMetazoa.
DR GO; GO:0010212; P:response to ionizing radiation; ISS:UniProtKB.
DR Gene3D; 3.40.50.10810; -; 1.
DR Gene3D; 3.40.50.300; -; 1.
DR InterPro; IPR014001; Helicase_ATP-bd.
DR InterPro; IPR001650; Helicase_C.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR013967; Rad54_N.
DR InterPro; IPR038718; SNF2-like_sf.
DR InterPro; IPR000330; SNF2_N.
DR Pfam; PF00271; Helicase_C; 1.
DR Pfam; PF08658; Rad54_N; 1.
DR Pfam; PF00176; SNF2-rel_dom; 1.
DR SMART; SM00487; DEXDc; 1.
DR SMART; SM00490; HELICc; 1.
DR SUPFAM; SSF52540; SSF52540; 2.
DR PROSITE; PS51192; HELICASE_ATP_BIND_1; 1.
DR PROSITE; PS51194; HELICASE_CTER; 1.
PE 3: Inferred from homology;
KW ATP-binding; Cell cycle; Cell division; DNA damage; DNA repair;
KW DNA-binding; Helicase; Hydrolase; Meiosis; Mitosis; Nucleotide-binding;
KW Nucleus; Phosphoprotein; Reference proteome.
FT CHAIN 1..786
FT /note="DNA repair and recombination protein RAD54-like"
FT /id="PRO_0000392521"
FT DOMAIN 165..340
FT /note="Helicase ATP-binding"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00541"
FT DOMAIN 497..654
FT /note="Helicase C-terminal"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00542"
FT REGION 2..9
FT /note="Required for chromatin remodeling, strand pairing
FT activities and coupling of ATPase activity"
FT /evidence="ECO:0000250|UniProtKB:O76460"
FT REGION 738..786
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOTIF 291..294
FT /note="DEGH box"
FT /evidence="ECO:0000255"
FT COMPBIAS 743..767
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT BINDING 178..185
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00541"
FT MOD_RES 22
FT /note="Phosphothreonine"
FT /evidence="ECO:0000250|UniProtKB:O76460"
SQ SEQUENCE 786 AA; 89188 MW; D17B11B357637500 CRC64;
MRRSLAPSQR LGVRIKSKDA FTPPLLKKNK RACQQELEKR QSALRDATNI VELPLPIRFT
ANSEYEQAIA KVLARKFKVP IANYVPDYGG NRSLGVRRSI VRRALHDPLA CNALVLFTPP
VYTEHERMSL DPSKLQVHVV VDPLLSNVLR PHQREGVRFM YECVEGKRGS FNGCIMADEM
GLGKTLQCVT LTWTLLRQSA DCKPTISKAI VVSPSSLVKN WEKEFTKWLH GRMHCLAMEG
GSKEETTRTL EQFAMNTSTR CGTPVLLISY ETFRLYAHIL CKTEVGMVIC DEGHRLKNSD
NLTYQALMGL KTKRRVLLSG TPIQNDLTEY FSLVNFVNPE MLGTATDFKR NFENAILRGQ
NADSTDTERE RALLKTQELI GLVNQCIIRR TNQILTKYLP VKFEMVVCAK LTAVQLQIYT
NFLKSDQVCR SLADCNEKTS LTALSDITTL KKLCNHPDLI YEKLAAREKG FENSQNVLPA
NYKPKDINPE LSGKFMLLDF MLAAIRANSD DKVVLISNYT QTLDLFEQLA RKRKYSYVRL
DGTMTIKKRS KVVDRFNDPS SDCFLFMLSS KAGGCGLNLI GANRLFMFDP DWNPANDEQA
MARVWRDGQK KPCYIYRLVA SGSIEEKILQ RQTHKKSLSS TIIDNNESVE KHFTRDDLKD
LFSFEANVLS DTHNKLKCKR CFQDVQRQPP ADNTDCTSHL SQWFHCSNNR GLPDTILSQA
WTASKCVSFV FHHRSQAEAK PAATTTDEDE ELSDSKRKAK KTLASDDDDD EDFVLNCSSG
EEFSGF
