RAD54_DROWI
ID RAD54_DROWI Reviewed; 784 AA.
AC B4MX21;
DT 23-MAR-2010, integrated into UniProtKB/Swiss-Prot.
DT 23-SEP-2008, sequence version 1.
DT 03-AUG-2022, entry version 69.
DE RecName: Full=DNA repair and recombination protein RAD54-like {ECO:0000250|UniProtKB:O76460};
DE EC=3.6.4.-;
DE AltName: Full=Protein okra {ECO:0000250|UniProtKB:O76460};
GN Name=okr {ECO:0000250|UniProtKB:O76460}; ORFNames=GK15573;
OS Drosophila willistoni (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7260;
RN [1] {ECO:0000312|EMBL:EDW76660.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Tucson 14030-0811.24 {ECO:0000312|EMBL:EDW76660.1};
RX PubMed=17994087; DOI=10.1038/nature06341;
RG Drosophila 12 genomes consortium;
RT "Evolution of genes and genomes on the Drosophila phylogeny.";
RL Nature 450:203-218(2007).
CC -!- FUNCTION: Involved in mitotic DNA repair and meiotic recombination.
CC Functions in the recombinational DNA repair pathway. Essential for
CC interhomolog gene conversion (GC), but may have a less important role
CC in intersister GC than spn-A/Rad51. In the presence of DNA, spn-A/Rad51
CC enhances the ATPase activity of okr/Rad54 (By similarity).
CC {ECO:0000250|UniProtKB:O76460}.
CC -!- SUBUNIT: Interacts (via N-terminus) with spn-A/Rad51. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250|UniProtKB:O76460}.
CC -!- SIMILARITY: Belongs to the SNF2/RAD54 helicase family. {ECO:0000255}.
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DR EMBL; CH963857; EDW76660.1; -; Genomic_DNA.
DR RefSeq; XP_002065674.1; XM_002065638.2.
DR AlphaFoldDB; B4MX21; -.
DR SMR; B4MX21; -.
DR STRING; 7260.FBpp0244716; -.
DR EnsemblMetazoa; FBtr0246224; FBpp0244716; FBgn0217578.
DR GeneID; 6642593; -.
DR KEGG; dwi:6642593; -.
DR eggNOG; KOG0390; Eukaryota.
DR HOGENOM; CLU_000315_10_2_1; -.
DR InParanoid; B4MX21; -.
DR OMA; YTEHERM; -.
DR OrthoDB; 93727at2759; -.
DR PhylomeDB; B4MX21; -.
DR ChiTaRS; okr; fly.
DR Proteomes; UP000007798; Unassembled WGS sequence.
DR GO; GO:0005634; C:nucleus; ISS:UniProtKB.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0140658; F:ATP-dependent chromatin remodeler activity; IEA:InterPro.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0004386; F:helicase activity; IEA:UniProtKB-KW.
DR GO; GO:0016817; F:hydrolase activity, acting on acid anhydrides; IEA:InterPro.
DR GO; GO:0051301; P:cell division; IEA:UniProtKB-KW.
DR GO; GO:0006338; P:chromatin remodeling; ISS:UniProtKB.
DR GO; GO:0043150; P:DNA synthesis involved in double-strand break repair via homologous recombination; ISS:UniProtKB.
DR GO; GO:0000724; P:double-strand break repair via homologous recombination; ISS:UniProtKB.
DR GO; GO:0045003; P:double-strand break repair via synthesis-dependent strand annealing; IEA:EnsemblMetazoa.
DR GO; GO:0000711; P:meiotic DNA repair synthesis; ISS:UniProtKB.
DR GO; GO:0030716; P:oocyte fate determination; IEA:EnsemblMetazoa.
DR GO; GO:0048477; P:oogenesis; IEA:EnsemblMetazoa.
DR GO; GO:0007131; P:reciprocal meiotic recombination; IEA:EnsemblMetazoa.
DR GO; GO:0010212; P:response to ionizing radiation; ISS:UniProtKB.
DR Gene3D; 3.40.50.10810; -; 1.
DR Gene3D; 3.40.50.300; -; 1.
DR InterPro; IPR014001; Helicase_ATP-bd.
DR InterPro; IPR001650; Helicase_C.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR013967; Rad54_N.
DR InterPro; IPR038718; SNF2-like_sf.
DR InterPro; IPR000330; SNF2_N.
DR Pfam; PF00271; Helicase_C; 1.
DR Pfam; PF08658; Rad54_N; 1.
DR Pfam; PF00176; SNF2-rel_dom; 1.
DR SMART; SM00487; DEXDc; 1.
DR SMART; SM00490; HELICc; 1.
DR SUPFAM; SSF52540; SSF52540; 2.
DR PROSITE; PS51192; HELICASE_ATP_BIND_1; 1.
DR PROSITE; PS51194; HELICASE_CTER; 1.
PE 3: Inferred from homology;
KW ATP-binding; Cell cycle; Cell division; DNA damage; DNA repair;
KW DNA-binding; Helicase; Hydrolase; Meiosis; Mitosis; Nucleotide-binding;
KW Nucleus; Phosphoprotein; Reference proteome.
FT CHAIN 1..784
FT /note="DNA repair and recombination protein RAD54-like"
FT /id="PRO_0000392527"
FT DOMAIN 169..344
FT /note="Helicase ATP-binding"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00541"
FT DOMAIN 501..658
FT /note="Helicase C-terminal"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00542"
FT REGION 2..9
FT /note="Required for chromatin remodeling, strand pairing
FT activities and coupling of ATPase activity"
FT /evidence="ECO:0000250|UniProtKB:O76460"
FT REGION 742..784
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOTIF 295..298
FT /note="DEGH box"
FT /evidence="ECO:0000255"
FT BINDING 182..189
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00541"
FT MOD_RES 22
FT /note="Phosphothreonine"
FT /evidence="ECO:0000250|UniProtKB:O76460"
SQ SEQUENCE 784 AA; 89597 MW; DD029F675583381F CRC64;
MRRSLAPSQR LGVRLQPNQE FTPPLLQKKN KRTCQQEQEQ LQCQFALRDA TNSNGSIPLP
IRFTANSEYE LAIAKVLARK FKVPIANYVP DYGGNRCLGV RRSIVRRALH DPQACNALVL
YTPPVYSEHE RMKMDPTKIL VHVVVDPLLS NILRPHQREG VRFMYDCVEG KKGNFNGCIM
ADEMGLGKTL QCVTLVWTLL RQSCECKPTI TKAIIVSPSS LVKNWEKEFT KWLHGRMHCL
AMEGGSKEDT IKALEQFSMN TSTRLGTPVL LISYETFRIY ANILCQNEVG MVICDEGHRL
KNSDNLTYQA LMGLKTKRRV LLSGTPIQND LTEYFSLVNF VNPEMLGTAA DFKRNFENPI
LKGQNTDSSD KERERALEKT QELIGLVNQC IIRRTNQILT KYLPVKFEMV ICVRLTSVQL
EFYTNFLKSD KVRRSLADCN EKASLTALAD ITTLKKLCSH PDLIYEKMLA RDKGFENSQN
ILPTNYKPKD LNPELSGKFM LLDFMLATIR ANSDDKVVLI SNYTQTLDLF EQLARKRKYT
FVRLDGTMTI KKRSKVVDRF NDPENDCFLF MLSSKAGGCG LNLIGANRLF MFDPDWNPAN
DEQAMARVWR DGQKKPCYIY RLVASGSIEE KILQRQTHKK SLSSTIIDNN ESAEKHFTRD
DLKDLFSFDS KILSDTHEKL KCKRCLQNVQ TKPPPEDTDC TSHLSQWFHC SNNRGLPDDI
LAQAWTASKC VSFVFHHRSQ AQAIKESEET KQEAEDTSIP AKSKRKRSTT PESDDCNDED
FKGF
