ID   RAD54_ORYSJ             Reviewed;         980 AA.
AC   A4PBL4; A0A0P0VPW7; B9F345; Q0DXB7; Q6Z6G1;
DT   05-SEP-2012, integrated into UniProtKB/Swiss-Prot.
DT   15-MAY-2007, sequence version 1.
DT   03-AUG-2022, entry version 75.
DE   RecName: Full=DNA repair and recombination protein RAD54;
DE            Short=OsRad54;
DE            EC=3.6.4.-;
GN   Name=RAD54; OrderedLocusNames=Os02g0762800, LOC_Os02g52510;
GN   ORFNames=OsJ_08484, P0486G03.23;
OS   Oryza sativa subsp. japonica (Rice).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC   Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX   NCBI_TaxID=39947;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], AND INTERACTION WITH MUS81.
RC   STRAIN=cv. Nipponbare;
RX   PubMed=17327258; DOI=10.1093/pcp/pcm029;
RA   Mimida N., Kitamoto H., Osakabe K., Nakashima M., Ito Y., Heyer W.D.,
RA   Toki S., Ichikawa H.;
RT   "Two alternatively spliced transcripts generated from OsMUS81, a rice
RT   homolog of yeast MUS81, are up-regulated by DNA-damaging treatments.";
RL   Plant Cell Physiol. 48:648-654(2007).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Nipponbare;
RX   PubMed=16100779; DOI=10.1038/nature03895;
RG   International rice genome sequencing project (IRGSP);
RT   "The map-based sequence of the rice genome.";
RL   Nature 436:793-800(2005).
RN   [3]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Nipponbare;
RX   PubMed=18089549; DOI=10.1093/nar/gkm978;
RG   The rice annotation project (RAP);
RT   "The rice annotation project database (RAP-DB): 2008 update.";
RL   Nucleic Acids Res. 36:D1028-D1033(2008).
RN   [4]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Nipponbare;
RX   PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA   Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA   Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA   Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA   Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT   "Improvement of the Oryza sativa Nipponbare reference genome using next
RT   generation sequence and optical map data.";
RL   Rice 6:4-4(2013).
RN   [5]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Nipponbare;
RX   PubMed=15685292; DOI=10.1371/journal.pbio.0030038;
RA   Yu J., Wang J., Lin W., Li S., Li H., Zhou J., Ni P., Dong W., Hu S.,
RA   Zeng C., Zhang J., Zhang Y., Li R., Xu Z., Li S., Li X., Zheng H., Cong L.,
RA   Lin L., Yin J., Geng J., Li G., Shi J., Liu J., Lv H., Li J., Wang J.,
RA   Deng Y., Ran L., Shi X., Wang X., Wu Q., Li C., Ren X., Wang J., Wang X.,
RA   Li D., Liu D., Zhang X., Ji Z., Zhao W., Sun Y., Zhang Z., Bao J., Han Y.,
RA   Dong L., Ji J., Chen P., Wu S., Liu J., Xiao Y., Bu D., Tan J., Yang L.,
RA   Ye C., Zhang J., Xu J., Zhou Y., Yu Y., Zhang B., Zhuang S., Wei H.,
RA   Liu B., Lei M., Yu H., Li Y., Xu H., Wei S., He X., Fang L., Zhang Z.,
RA   Zhang Y., Huang X., Su Z., Tong W., Li J., Tong Z., Li S., Ye J., Wang L.,
RA   Fang L., Lei T., Chen C.-S., Chen H.-C., Xu Z., Li H., Huang H., Zhang F.,
RA   Xu H., Li N., Zhao C., Li S., Dong L., Huang Y., Li L., Xi Y., Qi Q.,
RA   Li W., Zhang B., Hu W., Zhang Y., Tian X., Jiao Y., Liang X., Jin J.,
RA   Gao L., Zheng W., Hao B., Liu S.-M., Wang W., Yuan L., Cao M.,
RA   McDermott J., Samudrala R., Wang J., Wong G.K.-S., Yang H.;
RT   "The genomes of Oryza sativa: a history of duplications.";
RL   PLoS Biol. 3:266-281(2005).
CC   -!- FUNCTION: Involved in DNA repair and mitotic recombination.
CC       {ECO:0000250}.
CC   -!- SUBUNIT: Interacts with MUS81. {ECO:0000269|PubMed:17327258}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the SNF2/RAD54 helicase family. {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=BAD17217.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC       Sequence=BAF10121.2; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC       Sequence=EEE57852.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR   EMBL; AB240577; BAF56217.1; -; mRNA.
DR   EMBL; AP005002; BAD17217.1; ALT_SEQ; Genomic_DNA.
DR   EMBL; AP008208; BAF10121.2; ALT_SEQ; Genomic_DNA.
DR   EMBL; AP014958; BAS81046.1; -; Genomic_DNA.
DR   EMBL; CM000139; EEE57852.1; ALT_SEQ; Genomic_DNA.
DR   RefSeq; XP_015623131.1; XM_015767645.1.
DR   RefSeq; XP_015623132.1; XM_015767646.1.
DR   RefSeq; XP_015623133.1; XM_015767647.1.
DR   RefSeq; XP_015623134.1; XM_015767648.1.
DR   RefSeq; XP_015623135.1; XM_015767649.1.
DR   AlphaFoldDB; A4PBL4; -.
DR   SMR; A4PBL4; -.
DR   IntAct; A4PBL4; 1.
DR   STRING; 4530.OS02T0762800-00; -.
DR   PaxDb; A4PBL4; -.
DR   PRIDE; A4PBL4; -.
DR   EnsemblPlants; Os02t0762800-00; Os02t0762800-00; Os02g0762800.
DR   Gramene; Os02t0762800-00; Os02t0762800-00; Os02g0762800.
DR   KEGG; osa:4330819; -.
DR   eggNOG; KOG0390; Eukaryota.
DR   HOGENOM; CLU_000315_10_4_1; -.
DR   InParanoid; A4PBL4; -.
DR   OMA; FNDPSKD; -.
DR   OrthoDB; 93727at2759; -.
DR   Proteomes; UP000000763; Chromosome 2.
DR   Proteomes; UP000007752; Chromosome 2.
DR   Proteomes; UP000059680; Chromosome 2.
DR   Genevisible; A4PBL4; OS.
DR   GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0140658; F:ATP-dependent chromatin remodeler activity; IEA:InterPro.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0015616; F:DNA translocase activity; IBA:GO_Central.
DR   GO; GO:0004386; F:helicase activity; IEA:UniProtKB-KW.
DR   GO; GO:0016787; F:hydrolase activity; IEA:UniProtKB-KW.
DR   GO; GO:0051301; P:cell division; IEA:UniProtKB-KW.
DR   GO; GO:0045003; P:double-strand break repair via synthesis-dependent strand annealing; IBA:GO_Central.
DR   GO; GO:0007131; P:reciprocal meiotic recombination; IBA:GO_Central.
DR   Gene3D; 3.40.50.10810; -; 1.
DR   Gene3D; 3.40.50.300; -; 1.
DR   InterPro; IPR014001; Helicase_ATP-bd.
DR   InterPro; IPR001650; Helicase_C.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   InterPro; IPR038718; SNF2-like_sf.
DR   InterPro; IPR000330; SNF2_N.
DR   Pfam; PF00271; Helicase_C; 1.
DR   Pfam; PF00176; SNF2-rel_dom; 1.
DR   SMART; SM00487; DEXDc; 1.
DR   SMART; SM00490; HELICc; 1.
DR   SUPFAM; SSF52540; SSF52540; 2.
DR   PROSITE; PS51192; HELICASE_ATP_BIND_1; 1.
DR   PROSITE; PS51194; HELICASE_CTER; 1.
PE   1: Evidence at protein level;
KW   ATP-binding; Cell cycle; Cell division; DNA damage; DNA repair;
KW   DNA-binding; Helicase; Hydrolase; Mitosis; Nucleotide-binding; Nucleus;
KW   Reference proteome.
FT   CHAIN           1..980
FT                   /note="DNA repair and recombination protein RAD54"
FT                   /id="PRO_0000418426"
FT   DOMAIN          253..426
FT                   /note="Helicase ATP-binding"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00541"
FT   DOMAIN          593..758
FT                   /note="Helicase C-terminal"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00542"
FT   REGION          1..106
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          828..848
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          950..980
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   MOTIF           377..380
FT                   /note="DEAH box"
FT   COMPBIAS        17..52
FT                   /note="Acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        68..106
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        950..965
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        966..980
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   BINDING         266..273
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00541"
SQ   SEQUENCE   980 AA;  108302 MW;  92947F765AEFD8CD CRC64;
     MPSTSKCNRI SRVADEEEEE EIVAVSSDAD ESESESEVGS GAEEEDDDYV GESSDSAGGS
     GSGSGDGDGD EEGGRSDIGD GEGEGGGRRV RSACRGVRAN DRERKSQNVD ALVRGNLVVR
     RQPLIPRILS VSDAAAIARK PFKPPCQNGY SENNEQLARR LSARKRFVPW GSVQPFAVTN
     ILPQSPAVSS DDSVENEESL PPGIEPLILW QPEGRDKENS NFSAIKVDHL LVRYLRPHQR
     EGVQFMFDCV SGLLNDDGIS GCILADDMGL GKTLQSITLL YTLLCQGFDA KPMVKRAVVV
     TPTSLVSNWE SEIIKWLKGR VQLLALCEST RADVLSGIES FLKPLSRLQV LIVSYETFRM
     HSSKFERPGS CDLLICDEAH RLKNDQTLTN KALAALPCKR RILLSGTPMQ NDLEEFFSMV
     NFTNPGVLGD ATYFRRYYEA PIICGREPTA SAEEKNLGSE RSAELSAKVN LFILRRTNAL
     LSNHLPPKIV EVVCCKLTAL QTALYNHFIH SKNVKRLISE GTKQSKVLAY ITALKKLCNH
     PKLIYDTIKS NNSGGSGFDD CLRFFPPELF SGRSGSWTGG GGMWVELSGK MHVLARLLGH
     LRLKTDDRIV LVSNYTQTLD LFAQLCRERR YPYIRLDGAT SINKRQKLVN QFNDPSRDEF
     VFLLSSKAGG CGLNLVGGNR LILFDPDWNP ANDKQAAARV WRDGQKKRVY IYRFLSTGTI
     EEKVYQRQMS KEGLQKVIQQ EQADGKMQGS SLSTEDLRDL FTFHEQIRSE IHENLKCNRC
     NKDGCMVLDG SKFDSAATEH EASNSGENSY IDIGGFGAIS GCVQKMNSSN QQIGSPSEED
     LGSWGHHSDP STVPDTILQC SSGDEVSFVF TNQIDGKLVP VESMARAATH RTHEVTVNAE
     KEVGKINSSN VPGTERQSLL GKNLKMMGFN LKNSSMKFPT KSRRMLPNCL QGMNKTSTSS
     DHQQTKKLHV ISDASDDDFV