RAD55_YEAST
ID RAD55_YEAST Reviewed; 406 AA.
AC P38953; D6VS62;
DT 01-FEB-1995, integrated into UniProtKB/Swiss-Prot.
DT 01-FEB-1995, sequence version 1.
DT 03-AUG-2022, entry version 181.
DE RecName: Full=DNA repair protein RAD55;
GN Name=RAD55; OrderedLocusNames=YDR076W; ORFNames=D4426;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 204510 / AB320;
RX PubMed=8181742; DOI=10.1016/0378-1119(94)90362-x;
RA Lovett S.T.;
RT "Sequence of the RAD55 gene of Saccharomyces cerevisiae: similarity of
RT RAD55 to prokaryotic RecA and other RecA-like proteins.";
RL Gene 142:103-106(1994).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RA Kuwahara S., Yoshima T., Fong J., Ogawa T.;
RT "Cloning of the RAD55 gene and identification of its gene product in
RT Saccharomyces cerevisiae.";
RL Submitted (NOV-1996) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 96604 / S288c / FY1679;
RX PubMed=7483840; DOI=10.1002/yea.320110708;
RA Coster F., Jonniaux J.-L., Goffeau A.;
RT "Analysis of a 32.8 kb segment of yeast chromosome IV reveals 21 open
RT reading frames, including TPS2, PPH3, RAD55, SED1, PDC2, AFR1, SSS1, SLU7
RT and a tRNA for arginine.";
RL Yeast 11:673-679(1995).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=9169867;
RA Jacq C., Alt-Moerbe J., Andre B., Arnold W., Bahr A., Ballesta J.P.G.,
RA Bargues M., Baron L., Becker A., Biteau N., Bloecker H., Blugeon C.,
RA Boskovic J., Brandt P., Brueckner M., Buitrago M.J., Coster F.,
RA Delaveau T., del Rey F., Dujon B., Eide L.G., Garcia-Cantalejo J.M.,
RA Goffeau A., Gomez-Peris A., Granotier C., Hanemann V., Hankeln T.,
RA Hoheisel J.D., Jaeger W., Jimenez A., Jonniaux J.-L., Kraemer C.,
RA Kuester H., Laamanen P., Legros Y., Louis E.J., Moeller-Rieker S.,
RA Monnet A., Moro M., Mueller-Auer S., Nussbaumer B., Paricio N., Paulin L.,
RA Perea J., Perez-Alonso M., Perez-Ortin J.E., Pohl T.M., Prydz H.,
RA Purnelle B., Rasmussen S.W., Remacha M.A., Revuelta J.L., Rieger M.,
RA Salom D., Saluz H.P., Saiz J.E., Saren A.-M., Schaefer M., Scharfe M.,
RA Schmidt E.R., Schneider C., Scholler P., Schwarz S., Soler-Mira A.,
RA Urrestarazu L.A., Verhasselt P., Vissers S., Voet M., Volckaert G.,
RA Wagner G., Wambutt R., Wedler E., Wedler H., Woelfl S., Harris D.E.,
RA Bowman S., Brown D., Churcher C.M., Connor R., Dedman K., Gentles S.,
RA Hamlin N., Hunt S., Jones L., McDonald S., Murphy L.D., Niblett D.,
RA Odell C., Oliver K., Rajandream M.A., Richards C., Shore L., Walsh S.V.,
RA Barrell B.G., Dietrich F.S., Mulligan J.T., Allen E., Araujo R., Aviles E.,
RA Berno A., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M.,
RA Hunicke-Smith S., Hyman R.W., Komp C., Lashkari D., Lew H., Lin D.,
RA Mosedale D., Nakahara K., Namath A., Oefner P., Oh C., Petel F.X.,
RA Roberts D., Schramm S., Schroeder M., Shogren T., Shroff N., Winant A.,
RA Yelton M.A., Botstein D., Davis R.W., Johnston M., Andrews S., Brinkman R.,
RA Cooper J., Ding H., Du Z., Favello A., Fulton L., Gattung S., Greco T.,
RA Hallsworth K., Hawkins J., Hillier L.W., Jier M., Johnson D., Johnston L.,
RA Kirsten J., Kucaba T., Langston Y., Latreille P., Le T., Mardis E.,
RA Menezes S., Miller N., Nhan M., Pauley A., Peluso D., Rifkin L., Riles L.,
RA Taich A., Trevaskis E., Vignati D., Wilcox L., Wohldman P., Vaudin M.,
RA Wilson R., Waterston R., Albermann K., Hani J., Heumann K., Kleine K.,
RA Mewes H.-W., Zollner A., Zaccaria P.;
RT "The nucleotide sequence of Saccharomyces cerevisiae chromosome IV.";
RL Nature 387:75-78(1997).
RN [5]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [6]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=17322287; DOI=10.1101/gr.6037607;
RA Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F.,
RA Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J.,
RA Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J.,
RA Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D.,
RA LaBaer J.;
RT "Approaching a complete repository of sequence-verified protein-encoding
RT clones for Saccharomyces cerevisiae.";
RL Genome Res. 17:536-543(2007).
RN [7]
RP LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
RX PubMed=14562106; DOI=10.1038/nature02046;
RA Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N.,
RA O'Shea E.K., Weissman J.S.;
RT "Global analysis of protein expression in yeast.";
RL Nature 425:737-741(2003).
CC -!- FUNCTION: Required for radiation resistance and meiotic viability and
CC presumably acts in recombination and recombinational DNA repair
CC pathways.
CC -!- INTERACTION:
CC P38953; P25454: RAD51; NbExp=6; IntAct=EBI-14737, EBI-14709;
CC P38953; P25301: RAD57; NbExp=9; IntAct=EBI-14737, EBI-14744;
CC P38953; P12954: SRS2; NbExp=4; IntAct=EBI-14737, EBI-18110;
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}.
CC -!- MISCELLANEOUS: Present with 1050 molecules/cell in log phase SD medium.
CC {ECO:0000269|PubMed:14562106}.
CC -!- SIMILARITY: Belongs to the RecA family. RAD55 subfamily. {ECO:0000305}.
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DR EMBL; Z46796; CAA86798.1; -; Genomic_DNA.
DR EMBL; D10481; BAA01284.1; -; Genomic_DNA.
DR EMBL; U01144; AAA19688.1; -; Genomic_DNA.
DR EMBL; X82086; CAA57603.1; -; Genomic_DNA.
DR EMBL; Z74372; CAA98895.1; -; Genomic_DNA.
DR EMBL; AY723773; AAU09690.1; -; Genomic_DNA.
DR EMBL; BK006938; DAA11922.1; -; Genomic_DNA.
DR PIR; S48763; S48763.
DR RefSeq; NP_010361.3; NM_001180384.3.
DR AlphaFoldDB; P38953; -.
DR BioGRID; 32131; 272.
DR ComplexPortal; CPX-3139; RAD55-RAD57 complex.
DR DIP; DIP-1554N; -.
DR IntAct; P38953; 12.
DR MINT; P38953; -.
DR STRING; 4932.YDR076W; -.
DR iPTMnet; P38953; -.
DR MaxQB; P38953; -.
DR PaxDb; P38953; -.
DR PRIDE; P38953; -.
DR EnsemblFungi; YDR076W_mRNA; YDR076W; YDR076W.
DR GeneID; 851648; -.
DR KEGG; sce:YDR076W; -.
DR SGD; S000002483; RAD55.
DR VEuPathDB; FungiDB:YDR076W; -.
DR eggNOG; ENOG502RTRR; Eukaryota.
DR HOGENOM; CLU_053754_0_0_1; -.
DR InParanoid; P38953; -.
DR OMA; TIHELKC; -.
DR BioCyc; YEAST:G3O-29681-MON; -.
DR PRO; PR:P38953; -.
DR Proteomes; UP000002311; Chromosome IV.
DR RNAct; P38953; protein.
DR GO; GO:0033063; C:Rad51B-Rad51C-Rad51D-XRCC2 complex; IBA:GO_Central.
DR GO; GO:0033065; C:Rad51C-XRCC3 complex; IBA:GO_Central.
DR GO; GO:0005657; C:replication fork; IBA:GO_Central.
DR GO; GO:0033062; C:Rhp55-Rhp57 complex; IDA:SGD.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0008094; F:ATP-dependent activity, acting on DNA; ISS:SGD.
DR GO; GO:0140664; F:ATP-dependent DNA damage sensor activity; IEA:InterPro.
DR GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR GO; GO:0000730; P:DNA recombinase assembly; IMP:SGD.
DR GO; GO:0006302; P:double-strand break repair; IMP:ComplexPortal.
DR GO; GO:0042275; P:error-free postreplication DNA repair; IDA:ComplexPortal.
DR GO; GO:0030491; P:heteroduplex formation; IDA:SGD.
DR GO; GO:0000707; P:meiotic DNA recombinase assembly; IMP:SGD.
DR GO; GO:1903112; P:positive regulation of single-strand break repair via homologous recombination; IDA:ComplexPortal.
DR GO; GO:0007131; P:reciprocal meiotic recombination; IBA:GO_Central.
DR Gene3D; 3.40.50.300; -; 1.
DR InterPro; IPR013632; DNA_recomb/repair_Rad51_C.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR020588; RecA_ATP-bd.
DR Pfam; PF08423; Rad51; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
DR PROSITE; PS50162; RECA_2; 1.
PE 1: Evidence at protein level;
KW ATP-binding; DNA damage; DNA repair; Nucleotide-binding; Nucleus;
KW Reference proteome.
FT CHAIN 1..406
FT /note="DNA repair protein RAD55"
FT /id="PRO_0000122954"
FT REGION 385..406
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT BINDING 43..50
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255"
FT CONFLICT 110
FT /note="F -> L (in Ref. 2; AAA19688)"
FT /evidence="ECO:0000305"
FT CONFLICT 197
FT /note="F -> N (in Ref. 2; AAA19688)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 406 AA; 46350 MW; 8EA49831F7F3E098 CRC64;
MSLGIPLSQL IVESPKPLSS GITGLDEILN LGFQARSIYE IFGPPGIGKT NFGIQLVCNS
LEGIQQSEIN DDKILWIETF QEMPINILRE RFQKFKIVEE NVKRVRITKF GQLLYFFQNL
FKLSQSVRYK LVIIDGFSQL VCDHLCTLSK RGGGMIDKTI HELKCRHLIL IFTVMTKYTH
STGSTIIVLN DCMNTAFQSN EFESLEEYYE ILDDGSNFFV NSNNERRKNN VHILKSALVA
NIAMGSKDST WEVFLRDRIG LFRDWNEQVD ETVFVKSKRV KASSSQSNEG CTTIKEMRIN
KRNFENLRIA IVFNLHGEDR KREGRNLKRS RSSDDRNYIV KFDFDKATGQ LRDIIDLKPD
TANIASFPTL STSSSSCSQV FNNIDSNDNP LPNAEGKEEI IYDSEG