RAD61_YEAST
ID RAD61_YEAST Reviewed; 647 AA.
AC Q99359; D6VS00;
DT 31-OCT-2006, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1996, sequence version 1.
DT 03-AUG-2022, entry version 122.
DE RecName: Full=Protein RAD61;
DE AltName: Full=Radiation sensitivity protein 61;
GN Name=RAD61; OrderedLocusNames=YDR014W; ORFNames=PZB647;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=8896275;
RX DOI=10.1002/(sici)1097-0061(199609)12:10b<1085::aid-yea9>3.3.co;2-s;
RA Eide L.G., Sander C., Prydz H.;
RT "Sequencing and analysis of a 35.4 kb region on the right arm of chromosome
RT IV from Saccharomyces cerevisiae reveal 23 open reading frames.";
RL Yeast 12:1085-1090(1996).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=9169867;
RA Jacq C., Alt-Moerbe J., Andre B., Arnold W., Bahr A., Ballesta J.P.G.,
RA Bargues M., Baron L., Becker A., Biteau N., Bloecker H., Blugeon C.,
RA Boskovic J., Brandt P., Brueckner M., Buitrago M.J., Coster F.,
RA Delaveau T., del Rey F., Dujon B., Eide L.G., Garcia-Cantalejo J.M.,
RA Goffeau A., Gomez-Peris A., Granotier C., Hanemann V., Hankeln T.,
RA Hoheisel J.D., Jaeger W., Jimenez A., Jonniaux J.-L., Kraemer C.,
RA Kuester H., Laamanen P., Legros Y., Louis E.J., Moeller-Rieker S.,
RA Monnet A., Moro M., Mueller-Auer S., Nussbaumer B., Paricio N., Paulin L.,
RA Perea J., Perez-Alonso M., Perez-Ortin J.E., Pohl T.M., Prydz H.,
RA Purnelle B., Rasmussen S.W., Remacha M.A., Revuelta J.L., Rieger M.,
RA Salom D., Saluz H.P., Saiz J.E., Saren A.-M., Schaefer M., Scharfe M.,
RA Schmidt E.R., Schneider C., Scholler P., Schwarz S., Soler-Mira A.,
RA Urrestarazu L.A., Verhasselt P., Vissers S., Voet M., Volckaert G.,
RA Wagner G., Wambutt R., Wedler E., Wedler H., Woelfl S., Harris D.E.,
RA Bowman S., Brown D., Churcher C.M., Connor R., Dedman K., Gentles S.,
RA Hamlin N., Hunt S., Jones L., McDonald S., Murphy L.D., Niblett D.,
RA Odell C., Oliver K., Rajandream M.A., Richards C., Shore L., Walsh S.V.,
RA Barrell B.G., Dietrich F.S., Mulligan J.T., Allen E., Araujo R., Aviles E.,
RA Berno A., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M.,
RA Hunicke-Smith S., Hyman R.W., Komp C., Lashkari D., Lew H., Lin D.,
RA Mosedale D., Nakahara K., Namath A., Oefner P., Oh C., Petel F.X.,
RA Roberts D., Schramm S., Schroeder M., Shogren T., Shroff N., Winant A.,
RA Yelton M.A., Botstein D., Davis R.W., Johnston M., Andrews S., Brinkman R.,
RA Cooper J., Ding H., Du Z., Favello A., Fulton L., Gattung S., Greco T.,
RA Hallsworth K., Hawkins J., Hillier L.W., Jier M., Johnson D., Johnston L.,
RA Kirsten J., Kucaba T., Langston Y., Latreille P., Le T., Mardis E.,
RA Menezes S., Miller N., Nhan M., Pauley A., Peluso D., Rifkin L., Riles L.,
RA Taich A., Trevaskis E., Vignati D., Wilcox L., Wohldman P., Vaudin M.,
RA Wilson R., Waterston R., Albermann K., Hani J., Heumann K., Kleine K.,
RA Mewes H.-W., Zollner A., Zaccaria P.;
RT "The nucleotide sequence of Saccharomyces cerevisiae chromosome IV.";
RL Nature 387:75-78(1997).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [4]
RP SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
RX PubMed=14562095; DOI=10.1038/nature02026;
RA Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W.,
RA Weissman J.S., O'Shea E.K.;
RT "Global analysis of protein localization in budding yeast.";
RL Nature 425:686-691(2003).
RN [5]
RP LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
RX PubMed=14562106; DOI=10.1038/nature02046;
RA Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N.,
RA O'Shea E.K., Weissman J.S.;
RT "Global analysis of protein expression in yeast.";
RL Nature 425:737-741(2003).
RN [6]
RP FUNCTION.
RX PubMed=12816519; DOI=10.1667/rr3019;
RA Game J.C., Birrell G.W., Brown J.A., Shibata T., Baccari C., Chu A.M.,
RA Williamson M.S., Brown J.M.;
RT "Use of a genome-wide approach to identify new genes that control
RT resistance of Saccharomyces cerevisiae to ionizing radiation.";
RL Radiat. Res. 160:14-24(2003).
CC -!- FUNCTION: Involved in resistance to ionizing radiation.
CC {ECO:0000269|PubMed:12816519}.
CC -!- INTERACTION:
CC Q99359; P47037: SMC3; NbExp=9; IntAct=EBI-2082336, EBI-17423;
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:14562095}.
CC -!- MISCELLANEOUS: Present with 721 molecules/cell in log phase SD medium.
CC {ECO:0000269|PubMed:14562106}.
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DR EMBL; X95966; CAA65206.1; -; Genomic_DNA.
DR EMBL; Z48008; CAA88074.1; -; Genomic_DNA.
DR EMBL; Z74310; CAA98834.1; -; Genomic_DNA.
DR EMBL; BK006938; DAA11860.1; -; Genomic_DNA.
DR PIR; S50995; S50995.
DR RefSeq; NP_010297.1; NM_001180322.1.
DR AlphaFoldDB; Q99359; -.
DR SMR; Q99359; -.
DR BioGRID; 32065; 182.
DR IntAct; Q99359; 1.
DR MINT; Q99359; -.
DR STRING; 4932.YDR014W; -.
DR iPTMnet; Q99359; -.
DR MaxQB; Q99359; -.
DR PaxDb; Q99359; -.
DR PRIDE; Q99359; -.
DR EnsemblFungi; YDR014W_mRNA; YDR014W; YDR014W.
DR GeneID; 851577; -.
DR KEGG; sce:YDR014W; -.
DR SGD; S000002421; RAD61.
DR VEuPathDB; FungiDB:YDR014W; -.
DR eggNOG; ENOG502RY5C; Eukaryota.
DR HOGENOM; CLU_460918_0_0_1; -.
DR InParanoid; Q99359; -.
DR OMA; HLGLCLN; -.
DR BioCyc; YEAST:G3O-29633-MON; -.
DR PRO; PR:Q99359; -.
DR Proteomes; UP000002311; Chromosome IV.
DR RNAct; Q99359; protein.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0140588; P:chromatin looping; IMP:SGD.
DR GO; GO:0042138; P:meiotic DNA double-strand break formation; IMP:SGD.
DR GO; GO:0007064; P:mitotic sister chromatid cohesion; IGI:SGD.
DR GO; GO:1902340; P:negative regulation of chromosome condensation; IMP:SGD.
DR GO; GO:0034183; P:negative regulation of maintenance of mitotic sister chromatid cohesion; IMP:SGD.
DR GO; GO:1905339; P:positive regulation of cohesin unloading; IMP:SGD.
DR Gene3D; 1.25.10.60; -; 1.
DR InterPro; IPR031550; Rad61_Wapl.
DR InterPro; IPR038496; Rad61_Wapl_sf.
DR Pfam; PF16997; Wap1; 1.
PE 1: Evidence at protein level;
KW Nucleus; Reference proteome.
FT CHAIN 1..647
FT /note="Protein RAD61"
FT /id="PRO_0000255971"
FT REGION 1..90
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 17..50
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 67..90
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 647 AA; 74722 MW; ADCEFBA83CB49D95 CRC64;
MRAYGKRGPV LRTPFRSNKG LPSSSDVEFS DDDVNSVIPD VSSTISSSIA DHPIEGLLDE
PRKAQDSSSS FDGANEKPSS QLDSKRNDQN VKIITSSDTS MAFMKDEKLS AFNFLDGSKA
SKRKRRRTYQ KHDANITSSI EPDVQDEDSI TMHNEFESIR KIYNDINEFI LKLPRADDDI
LNKMLENEMK MDDSIENNSI RTSKDKKYGK FRTILINKNK ENEIMGEEVD QKANTLSLNN
ADNSNAEKEG LTSTNHYNEL KNMGDTIKYQ DDIEFLLSNS KSNDNTTVPI NEYFKKLLNL
SLMIINDEEF FQYAKRYFKK EIIKLSFAQF RSDFPELILL QGYLLHKVSE SQSDFPPSFD
NFSIELSKDD GKIRTKKNKH IKKLSHLNFE DFLRKTQFKT GLYYSLSLWE MHGNLSLDII
KRISILASNK DLFSRHVKTF IPLLEKLITA SEFCHMYIEQ PEMFDSLISN LNNQFKDMLD
DDSLIKILIL LTNMEVHNYT LWKEADMIFQ SSMNTILESI HPLTDAKVDN ILLHLGLCLN
ICSRENSRLK LDGKLWYDMK TIFVKMIRDG SDTENRLVQG LFYLNFSFLI KQRKENSNLD
PGELNLLLVE LEAFKSETSQ FNEGISNKIE IALNYLKSIY TSERITI
