RAD9B_HUMAN
ID RAD9B_HUMAN Reviewed; 426 AA.
AC Q6WBX8; Q5U5K0; Q6NVJ1; Q6ZVT7; Q8N7T9; Q96LI8;
DT 07-MAR-2006, integrated into UniProtKB/Swiss-Prot.
DT 28-NOV-2006, sequence version 2.
DT 03-AUG-2022, entry version 134.
DE RecName: Full=Cell cycle checkpoint control protein RAD9B;
DE AltName: Full=DNA repair exonuclease rad9 homolog B;
DE Short=hRAD9B;
GN Name=RAD9B;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), INTERACTION WITH HUS1; HUS1B; RAD1
RP AND RAD9A, AND TISSUE SPECIFICITY.
RC TISSUE=Testis;
RX PubMed=14500360;
RA Hopkins K.M., Wang X., Berlin A., Hang H., Thaker H.M., Lieberman H.B.;
RT "Expression of mammalian paralogues of HRAD9 and Mrad9 checkpoint control
RT genes in normal and cancerous testicular tissue.";
RL Cancer Res. 63:5291-5298(2003).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 2; 3 AND 4).
RC TISSUE=Testis;
RX PubMed=14702039; DOI=10.1038/ng1285;
RA Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H.,
RA Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M.,
RA Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K.,
RA Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T.,
RA Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M.,
RA Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S.,
RA Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H.,
RA Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K.,
RA Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N.,
RA Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y.,
RA Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K.,
RA Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T.,
RA Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T.,
RA Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y.,
RA Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H.,
RA Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y.,
RA Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H.,
RA Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O.,
RA Isogai T., Sugano S.;
RT "Complete sequencing and characterization of 21,243 full-length human
RT cDNAs.";
RL Nat. Genet. 36:40-45(2004).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=16541075; DOI=10.1038/nature04569;
RA Scherer S.E., Muzny D.M., Buhay C.J., Chen R., Cree A., Ding Y.,
RA Dugan-Rocha S., Gill R., Gunaratne P., Harris R.A., Hawes A.C.,
RA Hernandez J., Hodgson A.V., Hume J., Jackson A., Khan Z.M., Kovar-Smith C.,
RA Lewis L.R., Lozado R.J., Metzker M.L., Milosavljevic A., Miner G.R.,
RA Montgomery K.T., Morgan M.B., Nazareth L.V., Scott G., Sodergren E.,
RA Song X.-Z., Steffen D., Lovering R.C., Wheeler D.A., Worley K.C., Yuan Y.,
RA Zhang Z., Adams C.Q., Ansari-Lari M.A., Ayele M., Brown M.J., Chen G.,
RA Chen Z., Clerc-Blankenburg K.P., Davis C., Delgado O., Dinh H.H.,
RA Draper H., Gonzalez-Garay M.L., Havlak P., Jackson L.R., Jacob L.S.,
RA Kelly S.H., Li L., Li Z., Liu J., Liu W., Lu J., Maheshwari M.,
RA Nguyen B.-V., Okwuonu G.O., Pasternak S., Perez L.M., Plopper F.J.H.,
RA Santibanez J., Shen H., Tabor P.E., Verduzco D., Waldron L., Wang Q.,
RA Williams G.A., Zhang J., Zhou J., Allen C.C., Amin A.G., Anyalebechi V.,
RA Bailey M., Barbaria J.A., Bimage K.E., Bryant N.P., Burch P.E.,
RA Burkett C.E., Burrell K.L., Calderon E., Cardenas V., Carter K., Casias K.,
RA Cavazos I., Cavazos S.R., Ceasar H., Chacko J., Chan S.N., Chavez D.,
RA Christopoulos C., Chu J., Cockrell R., Cox C.D., Dang M., Dathorne S.R.,
RA David R., Davis C.M., Davy-Carroll L., Deshazo D.R., Donlin J.E.,
RA D'Souza L., Eaves K.A., Egan A., Emery-Cohen A.J., Escotto M., Flagg N.,
RA Forbes L.D., Gabisi A.M., Garza M., Hamilton C., Henderson N.,
RA Hernandez O., Hines S., Hogues M.E., Huang M., Idlebird D.G., Johnson R.,
RA Jolivet A., Jones S., Kagan R., King L.M., Leal B., Lebow H., Lee S.,
RA LeVan J.M., Lewis L.C., London P., Lorensuhewa L.M., Loulseged H.,
RA Lovett D.A., Lucier A., Lucier R.L., Ma J., Madu R.C., Mapua P.,
RA Martindale A.D., Martinez E., Massey E., Mawhiney S., Meador M.G.,
RA Mendez S., Mercado C., Mercado I.C., Merritt C.E., Miner Z.L., Minja E.,
RA Mitchell T., Mohabbat F., Mohabbat K., Montgomery B., Moore N., Morris S.,
RA Munidasa M., Ngo R.N., Nguyen N.B., Nickerson E., Nwaokelemeh O.O.,
RA Nwokenkwo S., Obregon M., Oguh M., Oragunye N., Oviedo R.J., Parish B.J.,
RA Parker D.N., Parrish J., Parks K.L., Paul H.A., Payton B.A., Perez A.,
RA Perrin W., Pickens A., Primus E.L., Pu L.-L., Puazo M., Quiles M.M.,
RA Quiroz J.B., Rabata D., Reeves K., Ruiz S.J., Shao H., Sisson I.,
RA Sonaike T., Sorelle R.P., Sutton A.E., Svatek A.F., Svetz L.A.,
RA Tamerisa K.S., Taylor T.R., Teague B., Thomas N., Thorn R.D., Trejos Z.Y.,
RA Trevino B.K., Ukegbu O.N., Urban J.B., Vasquez L.I., Vera V.A.,
RA Villasana D.M., Wang L., Ward-Moore S., Warren J.T., Wei X., White F.,
RA Williamson A.L., Wleczyk R., Wooden H.S., Wooden S.H., Yen J., Yoon L.,
RA Yoon V., Zorrilla S.E., Nelson D., Kucherlapati R., Weinstock G.,
RA Gibbs R.A.;
RT "The finished DNA sequence of human chromosome 12.";
RL Nature 440:346-351(2006).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 3 AND 5).
RC TISSUE=Testis;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [5]
RP INTERACTION WITH HUS1; HUS1B; RAD1 AND RAD17, AND TISSUE SPECIFICITY.
RX PubMed=14611806; DOI=10.1016/s0888-7543(03)00200-3;
RA Dufault V.M., Oestreich A.J., Vroman B.T., Karnitz L.M.;
RT "Identification and characterization of RAD9B, a paralog of the RAD9
RT checkpoint gene.";
RL Genomics 82:644-651(2003).
CC -!- SUBUNIT: Interacts with HUS1, HUS1B, RAD1, RAD9A and RAD17.
CC {ECO:0000269|PubMed:14500360, ECO:0000269|PubMed:14611806}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=5;
CC Name=5;
CC IsoId=Q6WBX8-5; Sequence=Displayed;
CC Name=1;
CC IsoId=Q6WBX8-1; Sequence=VSP_017446;
CC Name=2;
CC IsoId=Q6WBX8-2; Sequence=VSP_017445, VSP_017446;
CC Name=3;
CC IsoId=Q6WBX8-3; Sequence=VSP_017444, VSP_017446;
CC Name=4;
CC IsoId=Q6WBX8-4; Sequence=VSP_017444;
CC -!- TISSUE SPECIFICITY: Expressed in testis and skeletal muscle.
CC {ECO:0000269|PubMed:14500360, ECO:0000269|PubMed:14611806}.
CC -!- SIMILARITY: Belongs to the rad9 family. {ECO:0000305}.
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DR EMBL; AY297459; AAQ62859.1; -; mRNA.
DR EMBL; AK058176; BAB71704.1; -; mRNA.
DR EMBL; AK097665; BAC05138.1; -; mRNA.
DR EMBL; AK124109; BAC85774.1; -; mRNA.
DR EMBL; AC002350; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; BC047645; AAH47645.1; -; mRNA.
DR EMBL; BC068031; AAH68031.2; -; mRNA.
DR CCDS; CCDS73527.1; -. [Q6WBX8-2]
DR RefSeq; NP_001273460.1; NM_001286531.1. [Q6WBX8-2]
DR RefSeq; NP_001273461.1; NM_001286532.1. [Q6WBX8-2]
DR RefSeq; NP_001273462.1; NM_001286533.1. [Q6WBX8-3]
DR RefSeq; NP_001273463.1; NM_001286534.1. [Q6WBX8-3]
DR RefSeq; NP_001273464.1; NM_001286535.1.
DR RefSeq; NP_001273465.1; NM_001286536.1.
DR RefSeq; NP_689655.3; NM_152442.3.
DR RefSeq; XP_011536276.1; XM_011537974.2.
DR RefSeq; XP_016874367.1; XM_017018878.1.
DR RefSeq; XP_016874371.1; XM_017018882.1. [Q6WBX8-3]
DR AlphaFoldDB; Q6WBX8; -.
DR SMR; Q6WBX8; -.
DR BioGRID; 126872; 10.
DR CORUM; Q6WBX8; -.
DR STRING; 9606.ENSP00000376440; -.
DR iPTMnet; Q6WBX8; -.
DR PhosphoSitePlus; Q6WBX8; -.
DR BioMuta; RAD9B; -.
DR MassIVE; Q6WBX8; -.
DR PaxDb; Q6WBX8; -.
DR PeptideAtlas; Q6WBX8; -.
DR PRIDE; Q6WBX8; -.
DR ProteomicsDB; 67754; -. [Q6WBX8-5]
DR ProteomicsDB; 67755; -. [Q6WBX8-1]
DR ProteomicsDB; 67756; -. [Q6WBX8-2]
DR ProteomicsDB; 67757; -. [Q6WBX8-3]
DR ProteomicsDB; 67758; -. [Q6WBX8-4]
DR Antibodypedia; 31019; 87 antibodies from 20 providers.
DR DNASU; 144715; -.
DR Ensembl; ENST00000409246.5; ENSP00000387329.1; ENSG00000151164.19. [Q6WBX8-2]
DR Ensembl; ENST00000409425.5; ENSP00000386629.1; ENSG00000151164.19. [Q6WBX8-2]
DR GeneID; 144715; -.
DR KEGG; hsa:144715; -.
DR UCSC; uc001tre.6; human. [Q6WBX8-5]
DR CTD; 144715; -.
DR DisGeNET; 144715; -.
DR GeneCards; RAD9B; -.
DR HGNC; HGNC:21700; RAD9B.
DR HPA; ENSG00000151164; Tissue enriched (testis).
DR MalaCards; RAD9B; -.
DR MIM; 608368; gene.
DR neXtProt; NX_Q6WBX8; -.
DR OpenTargets; ENSG00000151164; -.
DR PharmGKB; PA134889252; -.
DR VEuPathDB; HostDB:ENSG00000151164; -.
DR eggNOG; KOG2810; Eukaryota.
DR GeneTree; ENSGT00390000005767; -.
DR InParanoid; Q6WBX8; -.
DR OMA; HTKIWSE; -.
DR OrthoDB; 1176140at2759; -.
DR PhylomeDB; Q6WBX8; -.
DR PathwayCommons; Q6WBX8; -.
DR Reactome; R-HSA-176187; Activation of ATR in response to replication stress.
DR Reactome; R-HSA-5685938; HDR through Single Strand Annealing (SSA).
DR Reactome; R-HSA-5693607; Processing of DNA double-strand break ends.
DR Reactome; R-HSA-5693616; Presynaptic phase of homologous DNA pairing and strand exchange.
DR Reactome; R-HSA-6804756; Regulation of TP53 Activity through Phosphorylation.
DR Reactome; R-HSA-69473; G2/M DNA damage checkpoint.
DR Reactome; R-HSA-9709570; Impaired BRCA2 binding to RAD51.
DR SignaLink; Q6WBX8; -.
DR BioGRID-ORCS; 144715; 41 hits in 1081 CRISPR screens.
DR ChiTaRS; RAD9B; human.
DR GenomeRNAi; 144715; -.
DR Pharos; Q6WBX8; Tdark.
DR PRO; PR:Q6WBX8; -.
DR Proteomes; UP000005640; Chromosome 12.
DR RNAct; Q6WBX8; protein.
DR Bgee; ENSG00000151164; Expressed in right testis and 100 other tissues.
DR ExpressionAtlas; Q6WBX8; baseline and differential.
DR Genevisible; Q6WBX8; HS.
DR GO; GO:0030896; C:checkpoint clamp complex; IBA:GO_Central.
DR GO; GO:0005654; C:nucleoplasm; TAS:Reactome.
DR GO; GO:0005634; C:nucleus; IDA:MGI.
DR GO; GO:0071479; P:cellular response to ionizing radiation; IBA:GO_Central.
DR GO; GO:0006281; P:DNA repair; IBA:GO_Central.
DR GO; GO:0000076; P:DNA replication checkpoint signaling; IBA:GO_Central.
DR GO; GO:0031573; P:mitotic intra-S DNA damage checkpoint signaling; IBA:GO_Central.
DR InterPro; IPR026584; Rad9.
DR InterPro; IPR007268; Rad9/Ddc1.
DR PANTHER; PTHR15237; PTHR15237; 1.
DR Pfam; PF04139; Rad9; 1.
DR PIRSF; PIRSF009303; Cell_cycle_RAD9; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Phosphoprotein; Reference proteome.
FT CHAIN 1..426
FT /note="Cell cycle checkpoint control protein RAD9B"
FT /id="PRO_0000226698"
FT MOD_RES 359
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q6WBX7"
FT VAR_SEQ 1..155
FT /note="Missing (in isoform 3 and isoform 4)"
FT /evidence="ECO:0000303|PubMed:14702039,
FT ECO:0000303|PubMed:15489334"
FT /id="VSP_017444"
FT VAR_SEQ 1..69
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:14702039"
FT /id="VSP_017445"
FT VAR_SEQ 409..426
FT /note="VCCRKEFNGSDAKYFCII -> GSFSIF (in isoform 1, isoform
FT 2 and isoform 3)"
FT /evidence="ECO:0000303|PubMed:14500360,
FT ECO:0000303|PubMed:14702039, ECO:0000303|PubMed:15489334"
FT /id="VSP_017446"
FT CONFLICT 226
FT /note="C -> Y (in Ref. 2; BAC05138)"
FT /evidence="ECO:0000305"
FT CONFLICT 401
FT /note="D -> G (in Ref. 2; BAB71704)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 426 AA; 47832 MW; 876E5F8E8F259A03 CRC64;
MLKCVMSGSQ VKVFGKAVQA LSRISDEFWL DPSKKGLALR CVNSSRSAYG CVLFSPVFFQ
HYQWSALVKM SENELDTTLH LKCKLGMKSI LPIFRCLNSL ERNIEKCRIF TRSDKCKVVI
QFFYRHGIKR THNICFQESQ PLQVIFDKNV CTNTLMIQPR LLADAIVLFT SSQEEVTLAV
TPLNFCLKSS NEESMDLSNA VHSEMFVGSD EFDFFQIGMD TEITFCFKEL KGILTFSEAT
HAPISIYFDF PGKPLALSID DMLVEANFIL ATLADEQSRA SSPQSLCLSQ KRKRSDLIEK
KAGKNVTGQA LECISKKAAP RRLYPKETLT NISALENCGS PAMKRVDGDV SEVSESSVSN
TEEVPGSLCL RKFSCMFFGA VSSDQQEHFN HPFDSLARAS DSEEDMNNVC CRKEFNGSDA
KYFCII
