RADA_HALSA
ID RADA_HALSA Reviewed; 343 AA.
AC Q9HMM4; O93747;
DT 27-APR-2001, integrated into UniProtKB/Swiss-Prot.
DT 27-APR-2001, sequence version 2.
DT 03-AUG-2022, entry version 117.
DE RecName: Full=DNA repair and recombination protein RadA;
GN Name=radA; Synonyms=radA1; OrderedLocusNames=VNG_2473G;
OS Halobacterium salinarum (strain ATCC 700922 / JCM 11081 / NRC-1)
OS (Halobacterium halobium).
OC Archaea; Euryarchaeota; Stenosarchaea group; Halobacteria; Halobacteriales;
OC Halobacteriaceae; Halobacterium.
OX NCBI_TaxID=64091;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=9922255; DOI=10.1128/jb.181.3.907-915.1999;
RA Sandler S.J., Hugenholtz P., Schleper C., DeLong E.F., Pace N.R.,
RA Clark A.J.;
RT "Diversity of radA genes from cultured and uncultured archaea: comparative
RT analysis of putative RadA proteins and their use as a phylogenetic
RT marker.";
RL J. Bacteriol. 181:907-915(1999).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 700922 / JCM 11081 / NRC-1;
RX PubMed=11016950; DOI=10.1073/pnas.190337797;
RA Ng W.V., Kennedy S.P., Mahairas G.G., Berquist B., Pan M., Shukla H.D.,
RA Lasky S.R., Baliga N.S., Thorsson V., Sbrogna J., Swartzell S., Weir D.,
RA Hall J., Dahl T.A., Welti R., Goo Y.A., Leithauser B., Keller K., Cruz R.,
RA Danson M.J., Hough D.W., Maddocks D.G., Jablonski P.E., Krebs M.P.,
RA Angevine C.M., Dale H., Isenbarger T.A., Peck R.F., Pohlschroder M.,
RA Spudich J.L., Jung K.-H., Alam M., Freitas T., Hou S., Daniels C.J.,
RA Dennis P.P., Omer A.D., Ebhardt H., Lowe T.M., Liang P., Riley M., Hood L.,
RA DasSarma S.;
RT "Genome sequence of Halobacterium species NRC-1.";
RL Proc. Natl. Acad. Sci. U.S.A. 97:12176-12181(2000).
CC -!- FUNCTION: Involved in DNA repair and in homologous recombination. Binds
CC and assemble on single-stranded DNA to form a nucleoprotein filament.
CC Hydrolyzes ATP in a ssDNA-dependent manner and promotes DNA strand
CC exchange between homologous DNA molecules (By similarity).
CC {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the eukaryotic RecA-like protein family.
CC {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAG20547.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR EMBL; AF090196; AAD16062.1; -; Genomic_DNA.
DR EMBL; AE004437; AAG20547.1; ALT_INIT; Genomic_DNA.
DR PIR; G84397; G84397.
DR PIR; T43802; T43802.
DR RefSeq; WP_012289511.1; NC_002607.1.
DR AlphaFoldDB; Q9HMM4; -.
DR SMR; Q9HMM4; -.
DR STRING; 64091.VNG_2473G; -.
DR PaxDb; Q9HMM4; -.
DR EnsemblBacteria; AAG20547; AAG20547; VNG_2473G.
DR GeneID; 5953925; -.
DR GeneID; 62887713; -.
DR KEGG; hal:VNG_2473G; -.
DR PATRIC; fig|64091.14.peg.1915; -.
DR HOGENOM; CLU_041732_0_0_2; -.
DR InParanoid; Q9HMM4; -.
DR OrthoDB; 30130at2157; -.
DR PhylomeDB; Q9HMM4; -.
DR Proteomes; UP000000554; Chromosome.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0140664; F:ATP-dependent DNA damage sensor activity; IEA:InterPro.
DR GO; GO:0003684; F:damaged DNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0006310; P:DNA recombination; IEA:UniProtKB-UniRule.
DR GO; GO:0006281; P:DNA repair; IEA:UniProtKB-UniRule.
DR CDD; cd01123; Rad51_DMC1_radA; 1.
DR Gene3D; 3.40.50.300; -; 1.
DR HAMAP; MF_00348; RadA_arch; 1.
DR InterPro; IPR003593; AAA+_ATPase.
DR InterPro; IPR013632; DNA_recomb/repair_Rad51_C.
DR InterPro; IPR011938; DNA_recomb/repair_RadA.
DR InterPro; IPR016467; DNA_recomb/repair_RecA-like.
DR InterPro; IPR010995; DNA_repair_Rad51/TF_NusA_a-hlx.
DR InterPro; IPR003583; Hlx-hairpin-Hlx_DNA-bd_motif.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR033925; Rad51_DMC1_RadA.
DR InterPro; IPR020588; RecA_ATP-bd.
DR InterPro; IPR020587; RecA_monomer-monomer_interface.
DR Pfam; PF08423; Rad51; 1.
DR PIRSF; PIRSF005856; Rad51; 1.
DR SMART; SM00382; AAA; 1.
DR SMART; SM00278; HhH1; 2.
DR SUPFAM; SSF47794; SSF47794; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
DR TIGRFAMs; TIGR02236; recomb_radA; 1.
DR PROSITE; PS50162; RECA_2; 1.
DR PROSITE; PS50163; RECA_3; 1.
PE 3: Inferred from homology;
KW ATP-binding; DNA damage; DNA recombination; DNA-binding;
KW Nucleotide-binding; Reference proteome.
FT CHAIN 1..343
FT /note="DNA repair and recombination protein RadA"
FT /id="PRO_0000150092"
FT BINDING 107..114
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255"
FT CONFLICT 1..2
FT /note="MP -> MRGAPPTRRYIS (in Ref. 1)"
FT /evidence="ECO:0000305"
FT CONFLICT 8
FT /note="E -> D (in Ref. 1; AAD16062)"
FT /evidence="ECO:0000305"
FT CONFLICT 15..17
FT /note="ATA -> PTT (in Ref. 1; AAD16062)"
FT /evidence="ECO:0000305"
FT CONFLICT 23
FT /note="N -> S (in Ref. 1; AAD16062)"
FT /evidence="ECO:0000305"
FT CONFLICT 30..32
FT /note="SLA -> ILT (in Ref. 1; AAD16062)"
FT /evidence="ECO:0000305"
FT CONFLICT 75
FT /note="R -> P (in Ref. 1; AAD16062)"
FT /evidence="ECO:0000305"
FT CONFLICT 132
FT /note="G -> A (in Ref. 1; AAD16062)"
FT /evidence="ECO:0000305"
FT CONFLICT 263
FT /note="K -> P (in Ref. 1; AAD16062)"
FT /evidence="ECO:0000305"
FT CONFLICT 266
FT /note="N -> D (in Ref. 1; AAD16062)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 343 AA; 37423 MW; 847D17052968EF2D CRC64;
MPESDLEELP GVGPATAEKL RDNGFDAFQS LAVANSAELS NTADIGESTA ADVIQAAREA
ADVGGFETGA TVLERREQIG KLTWNIPEVD DLLGGGVETQ SITEVYGEFG AGKSQVTHQL
AVNVQLPTEY GGLHGRAVFI DSEDTFRPER IDDMVRGLSD ETLQAAMEAH EIEGSTDDED
TLTELVDAFL DKIHVAKGFN SNHQMLLAEK AKEIASEHED GDWPVRMLTV DSLTAHFRAE
YVGRGELADR QQKLNKHLHD LEKVGNLYNA AVLVTNQVQS NPDAFFGDPT KPIGGNILGH
KSTFRMYLRK SKNDKRIVKL VDAPNLADGE AVMRVQDEGL KPE
