RADA_METMI
ID RADA_METMI Reviewed; 322 AA.
AC P0CW58; Q977P5;
DT 03-MAY-2011, integrated into UniProtKB/Swiss-Prot.
DT 03-MAY-2011, sequence version 1.
DT 03-AUG-2022, entry version 39.
DE RecName: Full=DNA repair and recombination protein RadA {ECO:0000255|HAMAP-Rule:MF_00348};
GN Name=radA {ECO:0000255|HAMAP-Rule:MF_00348};
OS Methanococcus maripaludis (Methanococcus deltae).
OC Archaea; Euryarchaeota; Methanomada group; Methanococci; Methanococcales;
OC Methanococcaceae; Methanococcus.
OX NCBI_TaxID=39152;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 43000 / DSM 2067 / JCM 10722 / JJ;
RA Reich C.I., McNeil L.K., Brace J.L., Brucker J.K., Olsen G.J.;
RT "Archaeal RecA homologs: different response to DNA damaging agents in
RT mesophilic and thermophilic Archaea.";
RL Submitted (NOV-2000) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Involved in DNA repair and in homologous recombination. Binds
CC and assemble on single-stranded DNA to form a nucleoprotein filament.
CC Hydrolyzes ATP in a ssDNA-dependent manner and promotes DNA strand
CC exchange between homologous DNA molecules. {ECO:0000255|HAMAP-
CC Rule:MF_00348}.
CC -!- SIMILARITY: Belongs to the eukaryotic RecA-like protein family.
CC {ECO:0000255|HAMAP-Rule:MF_00348}.
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DR EMBL; AF322003; AAL26913.1; -; Genomic_DNA.
DR PDB; 3ETL; X-ray; 2.40 A; A=1-322.
DR PDB; 3EW9; X-ray; 2.40 A; A=1-322.
DR PDB; 3EWA; X-ray; 2.00 A; A=1-322.
DR PDBsum; 3ETL; -.
DR PDBsum; 3EW9; -.
DR PDBsum; 3EWA; -.
DR AlphaFoldDB; P0CW58; -.
DR SMR; P0CW58; -.
DR BRENDA; 3.6.4.B7; 3262.
DR EvolutionaryTrace; P0CW58; -.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0140664; F:ATP-dependent DNA damage sensor activity; IEA:InterPro.
DR GO; GO:0003684; F:damaged DNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0006310; P:DNA recombination; IEA:UniProtKB-UniRule.
DR GO; GO:0006281; P:DNA repair; IEA:UniProtKB-UniRule.
DR CDD; cd01123; Rad51_DMC1_radA; 1.
DR Gene3D; 3.40.50.300; -; 1.
DR HAMAP; MF_00348; RadA_arch; 1.
DR InterPro; IPR003593; AAA+_ATPase.
DR InterPro; IPR013632; DNA_recomb/repair_Rad51_C.
DR InterPro; IPR011938; DNA_recomb/repair_RadA.
DR InterPro; IPR016467; DNA_recomb/repair_RecA-like.
DR InterPro; IPR010995; DNA_repair_Rad51/TF_NusA_a-hlx.
DR InterPro; IPR003583; Hlx-hairpin-Hlx_DNA-bd_motif.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR033925; Rad51_DMC1_RadA.
DR InterPro; IPR020588; RecA_ATP-bd.
DR InterPro; IPR020587; RecA_monomer-monomer_interface.
DR Pfam; PF08423; Rad51; 1.
DR PIRSF; PIRSF005856; Rad51; 1.
DR SMART; SM00382; AAA; 1.
DR SMART; SM00278; HhH1; 2.
DR SUPFAM; SSF47794; SSF47794; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
DR TIGRFAMs; TIGR02236; recomb_radA; 1.
DR PROSITE; PS50162; RECA_2; 1.
DR PROSITE; PS50163; RECA_3; 1.
PE 1: Evidence at protein level;
KW 3D-structure; ATP-binding; DNA damage; DNA recombination; DNA-binding;
KW Nucleotide-binding.
FT CHAIN 1..322
FT /note="DNA repair and recombination protein RadA"
FT /id="PRO_0000150097"
FT BINDING 105..112
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00348"
FT HELIX 5..7
FT /evidence="ECO:0007829|PDB:3EWA"
FT HELIX 13..21
FT /evidence="ECO:0007829|PDB:3EWA"
FT HELIX 27..31
FT /evidence="ECO:0007829|PDB:3EWA"
FT HELIX 35..39
FT /evidence="ECO:0007829|PDB:3EWA"
FT HELIX 46..59
FT /evidence="ECO:0007829|PDB:3EWA"
FT HELIX 68..74
FT /evidence="ECO:0007829|PDB:3EWA"
FT HELIX 85..90
FT /evidence="ECO:0007829|PDB:3EWA"
FT TURN 91..93
FT /evidence="ECO:0007829|PDB:3EWA"
FT STRAND 94..96
FT /evidence="ECO:0007829|PDB:3EWA"
FT STRAND 99..106
FT /evidence="ECO:0007829|PDB:3EWA"
FT HELIX 111..122
FT /evidence="ECO:0007829|PDB:3EWA"
FT HELIX 125..127
FT /evidence="ECO:0007829|PDB:3EWA"
FT TURN 132..134
FT /evidence="ECO:0007829|PDB:3EWA"
FT HELIX 137..139
FT /evidence="ECO:0007829|PDB:3EWA"
FT STRAND 140..152
FT /evidence="ECO:0007829|PDB:3EWA"
FT HELIX 156..166
FT /evidence="ECO:0007829|PDB:3EWA"
FT HELIX 170..175
FT /evidence="ECO:0007829|PDB:3EWA"
FT STRAND 177..181
FT /evidence="ECO:0007829|PDB:3EWA"
FT HELIX 185..200
FT /evidence="ECO:0007829|PDB:3EWA"
FT STRAND 204..214
FT /evidence="ECO:0007829|PDB:3EWA"
FT HELIX 215..220
FT /evidence="ECO:0007829|PDB:3EWA"
FT HELIX 224..226
FT /evidence="ECO:0007829|PDB:3EWA"
FT HELIX 227..247
FT /evidence="ECO:0007829|PDB:3EWA"
FT STRAND 251..256
FT /evidence="ECO:0007829|PDB:3EWA"
FT STRAND 258..260
FT /evidence="ECO:0007829|PDB:3EWA"
FT STRAND 270..274
FT /evidence="ECO:0007829|PDB:3EWA"
FT HELIX 275..281
FT /evidence="ECO:0007829|PDB:3EWA"
FT STRAND 283..290
FT /evidence="ECO:0007829|PDB:3EWA"
FT STRAND 295..302
FT /evidence="ECO:0007829|PDB:3EWA"
FT STRAND 304..306
FT /evidence="ECO:0007829|PDB:3EWA"
FT STRAND 309..315
FT /evidence="ECO:0007829|PDB:3EWA"
SQ SEQUENCE 322 AA; 35106 MW; 2FE5E2D3064E66DA CRC64;
MADVLTELPG VGPSTADKLI EGGYLDFMKI ATATIGELTD IEGISEKAAA KMIMAARDLC
DLGFKSGVEL LKQRQSVWRL STGSTELGTV LAGGIESQSV TEFAGMFGSG KTQIMHQTCV
NLQMREKIFA DLEGVVEEEL EAPKAVYIDT EGTFRPERVV QMAEGAGIDG QTVLDNTFVA
RAYNSDMQML FAEKIEDLIK GGNNIKLVII DSLTSTFRNE FTGRGKLAER QQKLGRHMAT
LNKLADLYNC IVLVTNQVAA KPDAYFGVAE QAIGGHVVGH AATFRFFLRK SKGDKRVAKL
YDSPHLPDSE AVFRITEKGI QD
