ID   RADA_PYRAE              Reviewed;         333 AA.
AC   Q8ZYR9;
DT   05-MAR-2002, integrated into UniProtKB/Swiss-Prot.
DT   05-MAR-2002, sequence version 1.
DT   03-AUG-2022, entry version 111.
DE   RecName: Full=DNA repair and recombination protein RadA {ECO:0000255|HAMAP-Rule:MF_00348};
GN   Name=radA {ECO:0000255|HAMAP-Rule:MF_00348}; OrderedLocusNames=PAE0654;
OS   Pyrobaculum aerophilum (strain ATCC 51768 / DSM 7523 / JCM 9630 / CIP
OS   104966 / NBRC 100827 / IM2).
OC   Archaea; Crenarchaeota; Thermoprotei; Thermoproteales; Thermoproteaceae;
OC   Pyrobaculum.
OX   NCBI_TaxID=178306;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ATCC 51768 / DSM 7523 / JCM 9630 / CIP 104966 / NBRC 100827 / IM2;
RX   PubMed=11371158; DOI=10.1006/jmbi.2001.4669;
RA   Slupska M.M., King A.G., Fitz-Gibbon S., Besemer J., Borodovsky M.,
RA   Miller J.H.;
RT   "Leaderless transcripts of the crenarchaeal hyperthermophile Pyrobaculum
RT   aerophilum.";
RL   J. Mol. Biol. 309:347-360(2001).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 51768 / DSM 7523 / JCM 9630 / CIP 104966 / NBRC 100827 / IM2;
RX   PubMed=11792869; DOI=10.1073/pnas.241636498;
RA   Fitz-Gibbon S.T., Ladner H., Kim U.-J., Stetter K.O., Simon M.I.,
RA   Miller J.H.;
RT   "Genome sequence of the hyperthermophilic crenarchaeon Pyrobaculum
RT   aerophilum.";
RL   Proc. Natl. Acad. Sci. U.S.A. 99:984-989(2002).
CC   -!- FUNCTION: Involved in DNA repair and in homologous recombination. Binds
CC       and assemble on single-stranded DNA to form a nucleoprotein filament.
CC       Hydrolyzes ATP in a ssDNA-dependent manner and promotes DNA strand
CC       exchange between homologous DNA molecules. {ECO:0000255|HAMAP-
CC       Rule:MF_00348}.
CC   -!- SIMILARITY: Belongs to the eukaryotic RecA-like protein family.
CC       {ECO:0000255|HAMAP-Rule:MF_00348}.
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DR   EMBL; AF354749; AAL73354.1; -; Genomic_DNA.
DR   EMBL; AE009441; AAL62924.1; -; Genomic_DNA.
DR   AlphaFoldDB; Q8ZYR9; -.
DR   SMR; Q8ZYR9; -.
DR   STRING; 178306.PAE0654; -.
DR   EnsemblBacteria; AAL62924; AAL62924; PAE0654.
DR   KEGG; pai:PAE0654; -.
DR   PATRIC; fig|178306.9.peg.470; -.
DR   eggNOG; arCOG00415; Archaea.
DR   HOGENOM; CLU_041732_0_0_2; -.
DR   InParanoid; Q8ZYR9; -.
DR   OMA; TFRIYLR; -.
DR   Proteomes; UP000002439; Chromosome.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0140664; F:ATP-dependent DNA damage sensor activity; IEA:InterPro.
DR   GO; GO:0003684; F:damaged DNA binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0006310; P:DNA recombination; IEA:UniProtKB-UniRule.
DR   GO; GO:0006281; P:DNA repair; IEA:UniProtKB-UniRule.
DR   CDD; cd01123; Rad51_DMC1_radA; 1.
DR   Gene3D; 3.40.50.300; -; 1.
DR   HAMAP; MF_00348; RadA_arch; 1.
DR   InterPro; IPR003593; AAA+_ATPase.
DR   InterPro; IPR013632; DNA_recomb/repair_Rad51_C.
DR   InterPro; IPR011938; DNA_recomb/repair_RadA.
DR   InterPro; IPR016467; DNA_recomb/repair_RecA-like.
DR   InterPro; IPR010995; DNA_repair_Rad51/TF_NusA_a-hlx.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   InterPro; IPR033925; Rad51_DMC1_RadA.
DR   InterPro; IPR020588; RecA_ATP-bd.
DR   InterPro; IPR020587; RecA_monomer-monomer_interface.
DR   Pfam; PF08423; Rad51; 1.
DR   PIRSF; PIRSF005856; Rad51; 1.
DR   SMART; SM00382; AAA; 1.
DR   SUPFAM; SSF47794; SSF47794; 1.
DR   SUPFAM; SSF52540; SSF52540; 1.
DR   TIGRFAMs; TIGR02236; recomb_radA; 1.
DR   PROSITE; PS50162; RECA_2; 1.
DR   PROSITE; PS50163; RECA_3; 1.
PE   3: Inferred from homology;
KW   ATP-binding; DNA damage; DNA recombination; DNA-binding;
KW   Nucleotide-binding; Reference proteome.
FT   CHAIN           1..333
FT                   /note="DNA repair and recombination protein RadA"
FT                   /id="PRO_0000150103"
FT   BINDING         127..134
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00348"
SQ   SEQUENCE   333 AA;  37164 MW;  C1278DB0CA0CF4C3 CRC64;
     MSSKKKKADA EVSQTQATVE VRADVDVEEL EGIGRVTGAK LKERGYYTVR DIAFASVKEL
     AEIIGNEDRA QQIIEAARKM LGLHSFISAL EVYERRKKIR RISTGVRSLD ELLGGGIETR
     AVTEIVGEFG SGKTQLCHQL AVMVQLPEER GGLGAKAIYI DTENTFRPER IMQIAKARGL
     DSDQALHNIF YARAYSSDHQ MILVEQAKSI IKQHNVALLV VDSVIAHFRS EFPGRENLAE
     RQQKLNKHVA DLLRLADAYD VAVVITNQVM AQPDVFFGNP LRPAGGNILA HGATYRLWLR
     KSKENIRIAK IFDSPYHPEG EVSFRITEEG LVD