ID   RADA_PYRIL              Reviewed;         330 AA.
AC   Q9UWR5; A1RSF9;
DT   27-APR-2001, integrated into UniProtKB/Swiss-Prot.
DT   03-APR-2007, sequence version 2.
DT   03-AUG-2022, entry version 118.
DE   RecName: Full=DNA repair and recombination protein RadA;
GN   Name=radA; OrderedLocusNames=Pisl_0713;
OS   Pyrobaculum islandicum (strain DSM 4184 / JCM 9189 / GEO3).
OC   Archaea; Crenarchaeota; Thermoprotei; Thermoproteales; Thermoproteaceae;
OC   Pyrobaculum.
OX   NCBI_TaxID=384616;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=10672022; DOI=10.1046/j.1432-1327.2000.01108.x;
RA   Spies M., Kil Y., Masui R., Kato R., Kujo C., Ohshima T., Kuramitsu S.,
RA   Lanzov V.;
RT   "The RadA protein from a hyperthermophilic archaeon Pyrobaculum islandicum
RT   is a DNA-dependent ATPase that exhibits two disparate catalytic modes, with
RT   a transition temperature at 75 degrees C.";
RL   Eur. J. Biochem. 267:1125-1137(2000).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=DSM 4184 / JCM 9189 / GEO3;
RA   Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C.,
RA   Glavina del Rio T., Dalin E., Tice H., Pitluck S., Meincke L., Brettin T.,
RA   Bruce D., Han C., Tapia R., Gilna P., Schmutz J., Larimer F., Land M.,
RA   Hauser L., Kyrpides N., Mikhailova N., Lowe T., Richardson P.;
RT   "Complete sequence of Pyrobaculum islandicum DSM 4184.";
RL   Submitted (DEC-2006) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Involved in DNA repair and in homologous recombination. Binds
CC       and assemble on single-stranded DNA to form a nucleoprotein filament.
CC       Hydrolyzes ATP in a ssDNA-dependent manner and promotes DNA strand
CC       exchange between homologous DNA molecules.
CC   -!- SIMILARITY: Belongs to the eukaryotic RecA-like protein family.
CC       {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=BAA88984.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR   EMBL; AB026115; BAA88984.1; ALT_INIT; Genomic_DNA.
DR   EMBL; CP000504; ABL87891.1; -; Genomic_DNA.
DR   RefSeq; WP_011762467.1; NC_008701.1.
DR   AlphaFoldDB; Q9UWR5; -.
DR   SMR; Q9UWR5; -.
DR   STRING; 384616.Pisl_0713; -.
DR   EnsemblBacteria; ABL87891; ABL87891; Pisl_0713.
DR   GeneID; 4617625; -.
DR   KEGG; pis:Pisl_0713; -.
DR   eggNOG; arCOG00415; Archaea.
DR   HOGENOM; CLU_041732_0_0_2; -.
DR   OMA; TFRIYLR; -.
DR   OrthoDB; 30130at2157; -.
DR   BRENDA; 3.6.4.B7; 5240.
DR   Proteomes; UP000002595; Chromosome.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0140664; F:ATP-dependent DNA damage sensor activity; IEA:InterPro.
DR   GO; GO:0003684; F:damaged DNA binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0006310; P:DNA recombination; IEA:UniProtKB-UniRule.
DR   GO; GO:0006281; P:DNA repair; IEA:UniProtKB-UniRule.
DR   CDD; cd01123; Rad51_DMC1_radA; 1.
DR   Gene3D; 3.40.50.300; -; 1.
DR   HAMAP; MF_00348; RadA_arch; 1.
DR   InterPro; IPR003593; AAA+_ATPase.
DR   InterPro; IPR013632; DNA_recomb/repair_Rad51_C.
DR   InterPro; IPR011938; DNA_recomb/repair_RadA.
DR   InterPro; IPR016467; DNA_recomb/repair_RecA-like.
DR   InterPro; IPR010995; DNA_repair_Rad51/TF_NusA_a-hlx.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   InterPro; IPR033925; Rad51_DMC1_RadA.
DR   InterPro; IPR020588; RecA_ATP-bd.
DR   InterPro; IPR020587; RecA_monomer-monomer_interface.
DR   Pfam; PF08423; Rad51; 1.
DR   PIRSF; PIRSF005856; Rad51; 1.
DR   SMART; SM00382; AAA; 1.
DR   SUPFAM; SSF47794; SSF47794; 1.
DR   SUPFAM; SSF52540; SSF52540; 1.
DR   TIGRFAMs; TIGR02236; recomb_radA; 1.
DR   PROSITE; PS50162; RECA_2; 1.
DR   PROSITE; PS50163; RECA_3; 1.
PE   3: Inferred from homology;
KW   ATP-binding; DNA damage; DNA recombination; DNA-binding;
KW   Nucleotide-binding.
FT   CHAIN           1..330
FT                   /note="DNA repair and recombination protein RadA"
FT                   /id="PRO_0000150105"
FT   BINDING         124..131
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255"
FT   CONFLICT        144
FT                   /note="P -> A (in Ref. 1; BAA88984)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   330 AA;  36799 MW;  32B20F8C9E046647 CRC64;
     MSSKKRKDAE VAQARVEITP DLDVEELEGV GRVTGAKLKE RGFFTVRDVA FASVKELAEI
     VGNEERAQQI VEAARKMLGL HSFVSALEVY ERRKKIRRIS TGVRALDELL GGGIETRAVT
     EVAGEFGSGK TQLCHQLAVM VQLPEERGGL GAKAIYIDTE NTFRPERIMQ IAKARGLDPD
     QALNNIFYAR AYSSDHQMIL VDQAKSIIRQ HNVALLIVDS VIAHFRSEFP GRENLAERQQ
     KLNKHVADLL RLADAYDVAV VITNQVMAQP DVFFGNPLRP AGGNILAHGA TYRLWLRKSK
     ENIRIAKIFD SPYHPEGEVS FRITEEGLVD