RADA_THEKO
ID RADA_THEKO Reviewed; 836 AA.
AC Q5JET4;
DT 30-AUG-2005, integrated into UniProtKB/Swiss-Prot.
DT 15-FEB-2005, sequence version 1.
DT 03-AUG-2022, entry version 109.
DE RecName: Full=DNA repair and recombination protein RadA;
DE Contains:
DE RecName: Full=Pko RadA intein;
GN Name=radA; OrderedLocusNames=TK1899;
OS Thermococcus kodakarensis (strain ATCC BAA-918 / JCM 12380 / KOD1)
OS (Pyrococcus kodakaraensis (strain KOD1)).
OC Archaea; Euryarchaeota; Thermococci; Thermococcales; Thermococcaceae;
OC Thermococcus.
OX NCBI_TaxID=69014;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC BAA-918 / JCM 12380 / KOD1;
RX PubMed=15710748; DOI=10.1101/gr.3003105;
RA Fukui T., Atomi H., Kanai T., Matsumi R., Fujiwara S., Imanaka T.;
RT "Complete genome sequence of the hyperthermophilic archaeon Thermococcus
RT kodakaraensis KOD1 and comparison with Pyrococcus genomes.";
RL Genome Res. 15:352-363(2005).
CC -!- FUNCTION: Involved in DNA repair and in homologous recombination. Binds
CC and assemble on single-stranded DNA to form a nucleoprotein filament.
CC Hydrolyzes ATP in a ssDNA-dependent manner and promotes DNA strand
CC exchange between homologous DNA molecules (By similarity).
CC {ECO:0000250}.
CC -!- PTM: This protein undergoes a protein self splicing that involves a
CC post-translational excision of the intervening region (intein) followed
CC by peptide ligation. {ECO:0000305}.
CC -!- MISCELLANEOUS: The intein interrupts the ATP-binding site.
CC -!- SIMILARITY: Belongs to the eukaryotic RecA-like protein family.
CC {ECO:0000305}.
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DR EMBL; AP006878; BAD86088.1; -; Genomic_DNA.
DR RefSeq; WP_011250850.1; NC_006624.1.
DR AlphaFoldDB; Q5JET4; -.
DR SMR; Q5JET4; -.
DR IntAct; Q5JET4; 1.
DR MINT; Q5JET4; -.
DR STRING; 69014.TK1899; -.
DR EnsemblBacteria; BAD86088; BAD86088; TK1899.
DR GeneID; 3233960; -.
DR KEGG; tko:TK1899; -.
DR PATRIC; fig|69014.16.peg.1857; -.
DR eggNOG; arCOG00415; Archaea.
DR eggNOG; arCOG03154; Archaea.
DR HOGENOM; CLU_349728_0_0_2; -.
DR InParanoid; Q5JET4; -.
DR OMA; KRTKASY; -.
DR OrthoDB; 30130at2157; -.
DR PhylomeDB; Q5JET4; -.
DR Proteomes; UP000000536; Chromosome.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0140664; F:ATP-dependent DNA damage sensor activity; IEA:InterPro.
DR GO; GO:0003684; F:damaged DNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0004519; F:endonuclease activity; IEA:UniProtKB-KW.
DR GO; GO:0006281; P:DNA repair; IEA:UniProtKB-UniRule.
DR GO; GO:0016539; P:intein-mediated protein splicing; IEA:InterPro.
DR GO; GO:0006314; P:intron homing; IEA:UniProtKB-KW.
DR CDD; cd01123; Rad51_DMC1_radA; 1.
DR Gene3D; 3.10.28.10; -; 1.
DR Gene3D; 3.40.50.300; -; 2.
DR HAMAP; MF_00348; RadA_arch; 1.
DR InterPro; IPR013632; DNA_recomb/repair_Rad51_C.
DR InterPro; IPR011938; DNA_recomb/repair_RadA.
DR InterPro; IPR010995; DNA_repair_Rad51/TF_NusA_a-hlx.
DR InterPro; IPR003586; Hint_dom_C.
DR InterPro; IPR003587; Hint_dom_N.
DR InterPro; IPR036844; Hint_dom_sf.
DR InterPro; IPR003583; Hlx-hairpin-Hlx_DNA-bd_motif.
DR InterPro; IPR027434; Homing_endonucl.
DR InterPro; IPR006142; INTEIN.
DR InterPro; IPR030934; Intein_C.
DR InterPro; IPR004042; Intein_endonuc.
DR InterPro; IPR006141; Intein_N.
DR InterPro; IPR004860; LAGLIDADG_2.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR033925; Rad51_DMC1_RadA.
DR InterPro; IPR020588; RecA_ATP-bd.
DR InterPro; IPR020587; RecA_monomer-monomer_interface.
DR Pfam; PF14528; LAGLIDADG_3; 1.
DR Pfam; PF08423; Rad51; 2.
DR PRINTS; PR00379; INTEIN.
DR SMART; SM00278; HhH1; 2.
DR SMART; SM00305; HintC; 1.
DR SMART; SM00306; HintN; 1.
DR SUPFAM; SSF47794; SSF47794; 1.
DR SUPFAM; SSF51294; SSF51294; 1.
DR SUPFAM; SSF52540; SSF52540; 2.
DR SUPFAM; SSF55608; SSF55608; 1.
DR TIGRFAMs; TIGR01443; intein_Cterm; 1.
DR TIGRFAMs; TIGR01445; intein_Nterm; 1.
DR TIGRFAMs; TIGR02236; recomb_radA; 1.
DR PROSITE; PS50818; INTEIN_C_TER; 1.
DR PROSITE; PS50819; INTEIN_ENDONUCLEASE; 1.
DR PROSITE; PS50817; INTEIN_N_TER; 1.
DR PROSITE; PS50162; RECA_2; 2.
DR PROSITE; PS50163; RECA_3; 1.
PE 3: Inferred from homology;
KW ATP-binding; Autocatalytic cleavage; DNA damage; DNA recombination;
KW DNA-binding; Endonuclease; Hydrolase; Intron homing; Nuclease;
KW Nucleotide-binding; Protein splicing; Reference proteome.
FT CHAIN 1..149
FT /note="DNA repair and recombination protein RadA, 1st part"
FT /evidence="ECO:0000255"
FT /id="PRO_0000030164"
FT CHAIN 150..631
FT /note="Pko RadA intein"
FT /evidence="ECO:0000255"
FT /id="PRO_0000030165"
FT CHAIN 632..836
FT /note="DNA repair and recombination protein RadA, 2nd part"
FT /evidence="ECO:0000255"
FT /id="PRO_0000030166"
FT DOMAIN 285..410
FT /note="DOD-type homing endonuclease"
FT REGION 15..41
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 24..41
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 836 AA; 93707 MW; 57387B98C8317F04 CRC64;
MARKKKVEDE VKELEEFEEL DVEESLSSSD KQSKPEKKIS ALEDLPGVGP ATAEKLREAG
YDTIEAIAVA SPLELKEIAG ISEGAALKII QAAREAANIG TFMRADEYMK RRTTIGKIST
GSKALDKLLG GGIETQAITE VFGEFGSGKC FAKDTKVYYE NDTLVHFESI EDMYHKYASL
GREVPFDNGY AVPLETVSVY TFDPKTGEVK RTKASYIYRE KVEKLAEIRL SNGYLLRITL
LHPVLVFRNG LQWVPAGMIK PGDLIVGIRS VPANAATIEE SEAYFLGLFV AEGTSNPLSI
TTGSEELKDF IVSFIEDHDG YTPTVEVRRG LYRILFRKKT AEWLGELATS NASTKVVPER
VLNAGESAIA AFLAGYLDGD GYLTESIVEL VTKSRELADG LVFLLKRLGI TPRISQKTIE
GSVYYRIYIT GEDRKTFEKV LEKSRIKPGE MNEGGVGRYP PALGKFLGKL YSEFRLPKRD
NETAYHILTR SRNVWFTEKT LSRIEEYFRE ALEKLSEARK ALEMGDKPEL PFPWTAITKY
GFTDRQVANY RTRGLPKRPE LKEKVVSALL KEIERLEGVA KLALETIELA RRLEFHEVSS
VEVVDYNDWV YDLVIPETHN FIAPNGLVLH NTQLAHTLAV MVQKPPEEGG LGGSVIWIDT
ENTFRPERIK QIAENRGLDP EETLKNIYVA RAFNSNHQML LVEKAEEIIK EKAESDRPVK
LLVVDSLMAH FRAEYVGRGT LAERQQKLAK HLADLHRLAD LYDIAVFVTN QVQAKPDAFF
GDPTRPVGGH ILAHSATLRV YLRKGKAGKR VARLIDSPHL PEGEAVFRIT EKGVED