ID   RADB_PYRFU              Reviewed;         225 AA.
AC   P81415;
DT   27-APR-2001, integrated into UniProtKB/Swiss-Prot.
DT   01-AUG-1998, sequence version 1.
DT   03-AUG-2022, entry version 127.
DE   RecName: Full=DNA repair and recombination protein RadB;
GN   Name=radB; OrderedLocusNames=PF0021;
OS   Pyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1).
OC   Archaea; Euryarchaeota; Thermococci; Thermococcales; Thermococcaceae;
OC   Pyrococcus.
OX   NCBI_TaxID=186497;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ATCC 43587 / DSM 3638 / JCM 8422 / Vc1;
RX   PubMed=9348040; DOI=10.1046/j.1365-2443.1997.1380336.x;
RA   Uemori T., Sato Y., Kato I., Doi H., Ishino Y.;
RT   "A novel DNA polymerase in the hyperthermophilic archaeon, Pyrococcus
RT   furiosus: gene cloning, expression, and characterization.";
RL   Genes Cells 2:499-512(1997).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ATCC 43587 / DSM 3638 / JCM 8422 / Vc1;
RX   PubMed=10486005; DOI=10.1007/pl00006570;
RA   DiRuggiero J., Brown J.R., Bogert A.P., Robb F.T.;
RT   "DNA repair systems in archaea: mementos from the last universal common
RT   ancestor?";
RL   J. Mol. Evol. 49:474-484(1999).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 43587 / DSM 3638 / JCM 8422 / Vc1;
RX   PubMed=10430560; DOI=10.1093/genetics/152.4.1299;
RA   Maeder D.L., Weiss R.B., Dunn D.M., Cherry J.L., Gonzalez J.M.,
RA   DiRuggiero J., Robb F.T.;
RT   "Divergence of the hyperthermophilic archaea Pyrococcus furiosus and P.
RT   horikoshii inferred from complete genomic sequences.";
RL   Genetics 152:1299-1305(1999).
RN   [4]
RP   CHARACTERIZATION.
RC   STRAIN=ATCC 43587 / DSM 3638 / JCM 8422 / Vc1;
RX   PubMed=10903318; DOI=10.1074/jbc.m004557200;
RA   Komori K., Miyata T., DiRuggiero J., Holley-Shanks R., Hayashi I.,
RA   Cann I.K.O., Mayanagi K., Shinagawa H., Ishino Y.;
RT   "Both RadA and RadB are involved in homologous recombination in Pyrococcus
RT   furiosus.";
RL   J. Biol. Chem. 275:33782-33790(2000).
CC   -!- FUNCTION: Involved in DNA repair and in homologous recombination. May
CC       regulate the cleavage reactions of the branch-structured DNA. Has a
CC       very weak ATPase activity that is not stimulated by DNA. Binds DNA but
CC       does not promote DNA strands exchange.
CC   -!- SIMILARITY: Belongs to the eukaryotic RecA-like protein family. RadB
CC       subfamily. {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=AAL80145.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR   EMBL; D84670; BAA25166.1; -; Genomic_DNA.
DR   EMBL; AF052598; AAC34999.1; -; Genomic_DNA.
DR   EMBL; AE009950; AAL80145.1; ALT_INIT; Genomic_DNA.
DR   PIR; T43936; T43936.
DR   AlphaFoldDB; P81415; -.
DR   SMR; P81415; -.
DR   STRING; 186497.PF0021; -.
DR   EnsemblBacteria; AAL80145; AAL80145; PF0021.
DR   KEGG; pfu:PF0021; -.
DR   PATRIC; fig|186497.12.peg.23; -.
DR   eggNOG; arCOG00417; Archaea.
DR   HOGENOM; CLU_041732_2_0_2; -.
DR   OMA; GNTLEHW; -.
DR   PhylomeDB; P81415; -.
DR   Proteomes; UP000001013; Chromosome.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0140664; F:ATP-dependent DNA damage sensor activity; IEA:InterPro.
DR   GO; GO:0003684; F:damaged DNA binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0006310; P:DNA recombination; IEA:UniProtKB-UniRule.
DR   GO; GO:0006281; P:DNA repair; IEA:UniProtKB-UniRule.
DR   Gene3D; 3.40.50.300; -; 1.
DR   HAMAP; MF_00350; RadB; 1.
DR   InterPro; IPR003593; AAA+_ATPase.
DR   InterPro; IPR013632; DNA_recomb/repair_Rad51_C.
DR   InterPro; IPR011939; DNA_repair_and_recomb_RadB.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   InterPro; IPR020588; RecA_ATP-bd.
DR   Pfam; PF08423; Rad51; 1.
DR   PIRSF; PIRSF003336; RadB; 1.
DR   SMART; SM00382; AAA; 1.
DR   SUPFAM; SSF52540; SSF52540; 1.
DR   TIGRFAMs; TIGR02237; recomb_radB; 1.
DR   PROSITE; PS50162; RECA_2; 1.
PE   1: Evidence at protein level;
KW   ATP-binding; DNA damage; DNA recombination; DNA-binding;
KW   Nucleotide-binding; Reference proteome.
FT   CHAIN           1..225
FT                   /note="DNA repair and recombination protein RadB"
FT                   /id="PRO_0000150117"
FT   BINDING         32..39
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   225 AA;  25339 MW;  E9003B0644436EBB CRC64;
     MLNTELLTTG VKGLDELLGG GVAKGVILQV YGPFATGKTT FAMQVGLLNE GKVAYVDTEG
     GFSPERLAQM AESRNLDVEK ALEKFVIFEP MDLNEQRQVI ARLKNIVNEK FSLVVVDSFT
     AHYRAEGSRE YGELSKQLQV LQWIARRKNV AVIVVNQVYY DSNSGILKPI AEHTLGYKTK
     DILRFERLRV GVRIAVLERH RFRPEGGMVY FKITDKGLED VKNED