RADX_MOUSE
ID RADX_MOUSE Reviewed; 850 AA.
AC Q8C779; A2AGL6; Q3TB47;
DT 31-OCT-2006, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2003, sequence version 1.
DT 03-AUG-2022, entry version 122.
DE RecName: Full=RPA-related protein RADX {ECO:0000250|UniProtKB:Q6NSI4};
GN Name=Radx {ECO:0000250|UniProtKB:Q6NSI4};
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
RC STRAIN=C57BL/6J; TISSUE=Fetal heart;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C57BL/6J;
RX PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA Eichler E.E., Ponting C.P.;
RT "Lineage-specific biology revealed by a finished genome assembly of the
RT mouse.";
RL PLoS Biol. 7:E1000112-E1000112(2009).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
CC -!- FUNCTION: Single-stranded DNA-binding protein recruited to replication
CC forks to maintain genome stability. Prevents fork collapse by
CC antagonizing the accumulation of RAD51 at forks to ensure the proper
CC balance of fork remodeling and protection without interfering with the
CC capacity of cells to complete homologous recombination of double-strand
CC breaks. {ECO:0000250|UniProtKB:Q6NSI4}.
CC -!- SUBCELLULAR LOCATION: Chromosome {ECO:0000250|UniProtKB:Q6NSI4}.
CC Note=Recruited to replication forks. {ECO:0000250|UniProtKB:Q6NSI4}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q8C779-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q8C779-2; Sequence=VSP_021184;
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DR EMBL; AK052376; BAC34963.1; -; mRNA.
DR EMBL; AK155588; BAE33336.1; -; mRNA.
DR EMBL; AK171459; BAE42467.1; -; mRNA.
DR EMBL; AL691424; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; BC113128; AAI13129.1; -; mRNA.
DR CCDS; CCDS30434.1; -. [Q8C779-1]
DR CCDS; CCDS85808.1; -. [Q8C779-2]
DR RefSeq; NP_001333451.1; NM_001346522.1. [Q8C779-2]
DR RefSeq; NP_780535.1; NM_175326.5. [Q8C779-1]
DR AlphaFoldDB; Q8C779; -.
DR SMR; Q8C779; -.
DR STRING; 10090.ENSMUSP00000108653; -.
DR iPTMnet; Q8C779; -.
DR PhosphoSitePlus; Q8C779; -.
DR MaxQB; Q8C779; -.
DR PaxDb; Q8C779; -.
DR PeptideAtlas; Q8C779; -.
DR PRIDE; Q8C779; -.
DR ProteomicsDB; 255073; -. [Q8C779-1]
DR ProteomicsDB; 255074; -. [Q8C779-2]
DR Antibodypedia; 348; 23 antibodies from 10 providers.
DR Ensembl; ENSMUST00000046763; ENSMUSP00000046045; ENSMUSG00000042498. [Q8C779-2]
DR Ensembl; ENSMUST00000113030; ENSMUSP00000108653; ENSMUSG00000042498. [Q8C779-1]
DR GeneID; 102871; -.
DR KEGG; mmu:102871; -.
DR UCSC; uc009ukg.2; mouse. [Q8C779-1]
DR UCSC; uc009ukh.2; mouse. [Q8C779-2]
DR CTD; 55086; -.
DR MGI; MGI:2147848; Radx.
DR VEuPathDB; HostDB:ENSMUSG00000042498; -.
DR eggNOG; ENOG502QSE7; Eukaryota.
DR GeneTree; ENSGT00390000005094; -.
DR HOGENOM; CLU_016770_0_0_1; -.
DR InParanoid; Q8C779; -.
DR OMA; KYLHHST; -.
DR OrthoDB; 218331at2759; -.
DR PhylomeDB; Q8C779; -.
DR TreeFam; TF331019; -.
DR BioGRID-ORCS; 102871; 1 hit in 73 CRISPR screens.
DR ChiTaRS; Radx; mouse.
DR PRO; PR:Q8C779; -.
DR Proteomes; UP000000589; Chromosome X.
DR RNAct; Q8C779; protein.
DR Bgee; ENSMUSG00000042498; Expressed in embryonic post-anal tail and 44 other tissues.
DR GO; GO:0016607; C:nuclear speck; ISO:MGI.
DR GO; GO:0005657; C:replication fork; ISS:UniProtKB.
DR GO; GO:0003697; F:single-stranded DNA binding; ISS:UniProtKB.
DR GO; GO:2000042; P:negative regulation of double-strand break repair via homologous recombination; ISS:UniProtKB.
DR GO; GO:0006282; P:regulation of DNA repair; IBA:GO_Central.
DR Gene3D; 2.40.50.140; -; 1.
DR InterPro; IPR012340; NA-bd_OB-fold.
DR InterPro; IPR040893; RADX.
DR PANTHER; PTHR14944; PTHR14944; 1.
DR Pfam; PF17659; DUF5521; 1.
DR SUPFAM; SSF50249; SSF50249; 2.
PE 2: Evidence at transcript level;
KW Alternative splicing; Chromosome; DNA-binding; Reference proteome.
FT CHAIN 1..850
FT /note="RPA-related protein RADX"
FT /id="PRO_0000254128"
FT DNA_BIND 228..331
FT /note="OB"
FT /evidence="ECO:0000250|UniProtKB:Q6NSI4"
FT REGION 1..31
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 575..612
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 632..671
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 589..608
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 641..671
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VAR_SEQ 471
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:16141072"
FT /id="VSP_021184"
FT CONFLICT 167
FT /note="P -> L (in Ref. 1; BAE42467)"
FT /evidence="ECO:0000305"
FT CONFLICT 331
FT /note="N -> D (in Ref. 1; BAE42467)"
FT /evidence="ECO:0000305"
FT CONFLICT 407
FT /note="I -> V (in Ref. 1; BAE42467)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 850 AA; 96847 MW; 66E24DF3EF74CB8E CRC64;
MSGESGQPQP GPSHAGLYLE HPERDQAGVP GGVIRRADSQ RRRSWIQKVI EQITGSPRQC
VTLSEVVPVT VLAVQRYLLE DEPRDTVPKP PLYCYDVTIS DGVYQEKCYL DPSLNFLVYQ
NILKVGIEMR ISRVSCLYNE KRLGQGILCI DKVHCGESLD VISVETPFRN RAHEEKPERP
LRGGKSHYLA LWNNEDPYGD IWKTNKQPEE FNFNNIKIIS LSHLELTWNS RKNFPALLVR
VLHKSKLRYY GKPNKKMIEP YQTYLEVADS SGMVSVILWN ALCPEWYKSL RVGLVLLLQD
YTVKKSYPLR IQPDPADPQM KLISTMEICL NLRDPPTNIV IIPEKQLKSE WKLPKLINRF
ITRSELDDMP EKSICDVIGL LSFVGRVQRS KKKENSEDFW SYRWIHITDG TSEQPFIVQL
FSTSQPEVFE NIYPMTYFVC TQLKVVRNNS QVPKLLYLTT TNESRALTTG GHRGLPYTYD
TKAKKIIQWI KTKTNLEAKN TVIGGYYPYP PVPETFSKYS RFIKAESLLT TISEVKKVIE
DLQYREQKRI AIQGIITAIK YIPYKHSAGS APAPEAFWNA SRPSTSQAAG KEDHCHERGS
KRSQDDRPMG SQHFQYTSKV LSLCAKRKIL QGPSANPVPV PQPHSSAQMK GSKHNTPSQE
SSTAYTTGKS RRITNDRWES QLWQDKKFSL RDHLHYGHVD PESIPRKFIL EHEKFLTQQF
NSQPAKYIPP EGKPPKLDEF QSARSLGHFE VTILGLNHEI AIDVAFLPMY SPEDVQASQI
DTFLTCMNFS CVYPPAAPLS GRLPDPKAVA GDIVKAAADL DRVHIIGILD ICNLGNNKVE
VCLQKIYTPE
