ID   RADX_RAT                Reviewed;         844 AA.
AC   B2GV47;
DT   05-MAY-2009, integrated into UniProtKB/Swiss-Prot.
DT   10-JUN-2008, sequence version 1.
DT   03-AUG-2022, entry version 71.
DE   RecName: Full=RPA-related protein RADX {ECO:0000250|UniProtKB:Q6NSI4};
GN   Name=Radx {ECO:0000250|UniProtKB:Q6NSI4};
OS   Rattus norvegicus (Rat).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC   Murinae; Rattus.
OX   NCBI_TaxID=10116;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Brown Norway;
RA   Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.;
RL   Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Testis;
RX   PubMed=15489334; DOI=10.1101/gr.2596504;
RG   The MGC Project Team;
RT   "The status, quality, and expansion of the NIH full-length cDNA project:
RT   the Mammalian Gene Collection (MGC).";
RL   Genome Res. 14:2121-2127(2004).
CC   -!- FUNCTION: Single-stranded DNA-binding protein recruited to replication
CC       forks to maintain genome stability. Prevents fork collapse by
CC       antagonizing the accumulation of RAD51 at forks to ensure the proper
CC       balance of fork remodeling and protection without interfering with the
CC       capacity of cells to complete homologous recombination of double-strand
CC       breaks. {ECO:0000250|UniProtKB:Q6NSI4}.
CC   -!- SUBCELLULAR LOCATION: Chromosome {ECO:0000250|UniProtKB:Q6NSI4}.
CC       Note=Recruited to replication forks. {ECO:0000250|UniProtKB:Q6NSI4}.
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DR   EMBL; CH474076; EDL88019.1; -; Genomic_DNA.
DR   EMBL; BC166523; AAI66523.1; -; mRNA.
DR   RefSeq; NP_001120849.1; NM_001127377.1.
DR   AlphaFoldDB; B2GV47; -.
DR   STRING; 10116.ENSRNOP00000014811; -.
DR   iPTMnet; B2GV47; -.
DR   PhosphoSitePlus; B2GV47; -.
DR   PaxDb; B2GV47; -.
DR   PRIDE; B2GV47; -.
DR   GeneID; 680663; -.
DR   KEGG; rno:680663; -.
DR   UCSC; RGD:1589413; rat.
DR   CTD; 55086; -.
DR   RGD; 1589413; Radx.
DR   VEuPathDB; HostDB:ENSRNOG00000011120; -.
DR   eggNOG; ENOG502QSE7; Eukaryota.
DR   HOGENOM; CLU_016770_0_0_1; -.
DR   InParanoid; B2GV47; -.
DR   OMA; KYLHHST; -.
DR   OrthoDB; 218331at2759; -.
DR   PhylomeDB; B2GV47; -.
DR   PRO; PR:B2GV47; -.
DR   Proteomes; UP000002494; Chromosome X.
DR   Proteomes; UP000234681; Chromosome x.
DR   Bgee; ENSRNOG00000011120; Expressed in duodenum and 4 other tissues.
DR   GO; GO:0016607; C:nuclear speck; IEA:Ensembl.
DR   GO; GO:0005657; C:replication fork; ISS:UniProtKB.
DR   GO; GO:0003697; F:single-stranded DNA binding; ISS:UniProtKB.
DR   GO; GO:2000042; P:negative regulation of double-strand break repair via homologous recombination; ISS:UniProtKB.
DR   GO; GO:0006282; P:regulation of DNA repair; IBA:GO_Central.
DR   Gene3D; 2.40.50.140; -; 1.
DR   InterPro; IPR012340; NA-bd_OB-fold.
DR   InterPro; IPR040893; RADX.
DR   PANTHER; PTHR14944; PTHR14944; 1.
DR   Pfam; PF17659; DUF5521; 1.
DR   SUPFAM; SSF50249; SSF50249; 2.
PE   2: Evidence at transcript level;
KW   Chromosome; DNA-binding; Reference proteome.
FT   CHAIN           1..844
FT                   /note="RPA-related protein RADX"
FT                   /id="PRO_0000373802"
FT   DNA_BIND        228..331
FT                   /note="OB"
FT                   /evidence="ECO:0000250|UniProtKB:Q6NSI4"
FT   REGION          571..609
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          626..664
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        571..587
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        590..607
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        635..664
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   844 AA;  96287 MW;  78F3AC219687581B CRC64;
     MSGESDQPQP GPSHAGLYLE HRERDQAGVP GGVIRRAGSQ RHRSWIQTVI EQITGSPRQC
     VTLSEVVPVS VLAVQRYLLE DEPRDTVPKP PLYCYDVTIS DGVYQEKCYL DPSLNFLVYQ
     NILKVGIEMR ISRVSCLYNE KRLGQGILCI DKVHCGEPLD VISVETPFRN RAHEEKPERP
     LRGSKSHYLA LWNNEDPYGD IWKTNKQPEE FNFNNTKIIS LSHLEMTWHN RKNFPALLVR
     ILHKSKLRYY GKPNKKMIEP YQTYLEVADS SGMVSVIMWN ALCPEWYKSL RVGLILLLQD
     YSVKKSYPLR IQPDPVDPQM KLISTMEICL NLRDPPTNIV IIPEKQLKSE WKLPKLINRF
     ITRSELDDMP ENSICDVIGM LSFVGRVQRS KKKENSEDFW SYRWIHITDG TSEQPFIVQL
     FSTSQPEVFE NIYPMTYFVC TQLKVVRNNS QVPKLLYLTT TNESRVLITG HRGQPYTYDT
     KAKKIIQWIK TKTNLEAKNT VIGGYYPYPP VPETFSKYSR FIKAESLLTT ISEVRKVIED
     LQYREQKRIA IQGIITAIKY IPYNHSAKSA PASETLQNAS PPSTSQAAAK EGHYHERGSK
     RSQDDRPMDS LPMVLSLCAK RKILQGPTAN PVPVPQPHSS AQMKGNKPNI PSRENSTANA
     TGKSKRIIND RWESQLWRDK KFSLRDHLHY GHVDPESIPR KFILGHEKFL TQQFNSQPAK
     YVPPEGKPPK LDEFQSARSL GHFEVTILGL NHEIAIDVAF LPMYSPEDVE TSQIDTFLTC
     MNYSCVYPPA APVSGRVPDP KAVAGDIVKA AADLDRVHII GILDICNLGN NKVEVCLQKI
     YTPE