ID   RAFP_PEDPE              Reviewed;         641 AA.
AC   P43466;
DT   01-NOV-1995, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1995, sequence version 1.
DT   25-MAY-2022, entry version 98.
DE   RecName: Full=Raffinose carrier protein;
DE   AltName: Full=Raffinose permease;
DE   Includes:
DE     RecName: Full=Putative phosphotransferase enzyme EIIA component;
DE              EC=2.7.1.-;
DE     AltName: Full=Putative PTS system EIIA component;
GN   Name=rafP;
OS   Pediococcus pentosaceus.
OC   Bacteria; Firmicutes; Bacilli; Lactobacillales; Lactobacillaceae;
OC   Pediococcus.
OX   NCBI_TaxID=1255;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=PPE1.0;
RA   Leenhouts K.K.J., Bolhuis A.A., Kok J.J., Venema G.G.;
RT   "The sucrose and raffinose operons of Pediococcus pentosaceus PPE1.0.";
RL   Submitted (APR-1994) to the EMBL/GenBank/DDBJ databases.
CC   -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Multi-pass membrane
CC       protein {ECO:0000305}.
CC   -!- DOMAIN: The PTS EIIA type-1 domain may serve a regulatory function,
CC       through its phosphorylation activity. {ECO:0000250}.
CC   -!- SIMILARITY: In the N-terminal section; belongs to the
CC       sodium:galactoside symporter (TC 2.A.2) family. {ECO:0000305}.
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DR   EMBL; Z32771; CAA83664.1; -; Genomic_DNA.
DR   EMBL; L32093; AAA25563.1; -; Genomic_DNA.
DR   PIR; S44253; S44253.
DR   AlphaFoldDB; P43466; -.
DR   SMR; P43466; -.
DR   TCDB; 2.A.2.2.2; the glycoside-pentoside-hexuronide (gph):cation symporter family.
DR   PRIDE; P43466; -.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0015293; F:symporter activity; IEA:UniProtKB-KW.
DR   GO; GO:0016740; F:transferase activity; IEA:UniProtKB-KW.
DR   GO; GO:0009401; P:phosphoenolpyruvate-dependent sugar phosphotransferase system; IEA:InterPro.
DR   GO; GO:0006814; P:sodium ion transport; IEA:InterPro.
DR   Gene3D; 1.20.1250.20; -; 2.
DR   Gene3D; 2.70.70.10; -; 1.
DR   InterPro; IPR011055; Dup_hybrid_motif.
DR   InterPro; IPR039672; MFS_2.
DR   InterPro; IPR036259; MFS_trans_sf.
DR   InterPro; IPR001927; Na/Gal_symport.
DR   InterPro; IPR018043; Na/Gal_symport_CS.
DR   InterPro; IPR001127; PTS_EIIA_1_perm.
DR   PANTHER; PTHR11328; PTHR11328; 1.
DR   Pfam; PF00358; PTS_EIIA_1; 1.
DR   SUPFAM; SSF103473; SSF103473; 1.
DR   SUPFAM; SSF51261; SSF51261; 1.
DR   TIGRFAMs; TIGR00792; gph; 1.
DR   TIGRFAMs; TIGR00830; PTBA; 1.
DR   PROSITE; PS00872; NA_GALACTOSIDE_SYMP; 1.
DR   PROSITE; PS51093; PTS_EIIA_TYPE_1; 1.
DR   PROSITE; PS00371; PTS_EIIA_TYPE_1_HIS; 1.
PE   3: Inferred from homology;
KW   Cell membrane; Membrane; Phosphoprotein; Sugar transport; Symport;
KW   Transferase; Transmembrane; Transmembrane helix; Transport.
FT   CHAIN           1..641
FT                   /note="Raffinose carrier protein"
FT                   /id="PRO_0000170761"
FT   TRANSMEM        25..45
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        57..77
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        93..113
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        120..140
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        168..188
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        201..221
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        253..273
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        288..308
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        317..337
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        342..362
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        394..414
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        429..449
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   DOMAIN          507..611
FT                   /note="PTS EIIA type-1"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00416"
FT   REGION          1..506
FT                   /note="Permease"
FT   MOD_RES         559
FT                   /note="Phosphohistidine; by HPr"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   641 AA;  69914 MW;  15BEC5F69F8C0F61 CRC64;
     MQEEHNYKWV GGRLIYGFGA KGNDAFYSIL SGYLIIFITS HLFDTGNKAL DNRMVSLVTL
     IIMVLRIVEL FIDPFIGNAI DRTKNSPGHF RPWVVVGGTV SSIILLLLFT NLGGLYAKNA
     MIYLVVFAIL YITMDIFYSF KDVGFWSMLP SLTTDSRERE KTATFARLGS TIGGGLVGVL
     VMPAVIFFSA KATSTGDNRG WFIFALIICL IALISAWGVG LGTREVDSDI RKNKQDTVGV
     MEIFKALAKN DQLLWAALAY LFYGVGINIL GSLEVYYFTY IMGKPKSFSI LSIINIFLGL
     IATSLFPVLS KKFSRKGVFA GCLVFMLGGI AIFTIAGSNL WLVLLAATMF GFPQQMVFLV
     VLMVITDSVE YGQLKLGHRD ESLALSVRPL IDKFGGAISN GVVGQIAIIS GMTTGATASS
     ITAAGQLHFK LTMFAFPALM LLIAIGIFSK QIFLTEEKHA EIVAELERTW RTKFDNTTDQ
     VAEKVVTSLD LATPIAGQVI PLAQVNDPTF AAGTLGDGFA IKPSDGRILA PFDATVRQVF
     TTRHAVGLVG DNGIVLLIHI GLGTVKLRGT GFISYVEEGQ HVQQGDELLE FWDPTIKQAG
     LDDTVIMTVT NSTEFTMMDW LVKPGQAVKA TDNILQLHTK A