ID   RAFR_ECOLX              Reviewed;         336 AA.
AC   P21867;
DT   01-MAY-1991, integrated into UniProtKB/Swiss-Prot.
DT   23-JAN-2007, sequence version 3.
DT   25-MAY-2022, entry version 90.
DE   RecName: Full=HTH-type transcriptional regulator RafR;
DE   AltName: Full=Raffinose operon repressor;
GN   Name=rafR;
OS   Escherichia coli.
OG   Plasmid pRSD2.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=562;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND PROTEIN SEQUENCE OF 2-6.
RX   PubMed=2180920; DOI=10.1128/jb.172.4.2178-2180.1990;
RA   Aslanidis C., Schmitt R.;
RT   "Regulatory elements of the raffinose operon: nucleotide sequences of
RT   operator and repressor genes.";
RL   J. Bacteriol. 172:2178-2180(1990).
CC   -!- FUNCTION: The raffinose (raf) operon is negatively controlled by the
CC       specific binding of raf repressor to raf operator (rafO) DNA. Binds
CC       raffinose as an inducer.
CC   -!- SUBUNIT: Homotetramer.
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DR   EMBL; M29849; AAA24500.1; -; Genomic_DNA.
DR   PIR; A35160; A35160.
DR   AlphaFoldDB; P21867; -.
DR   SMR; P21867; -.
DR   PRIDE; P21867; -.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0006355; P:regulation of transcription, DNA-templated; IEA:InterPro.
DR   CDD; cd01392; HTH_LacI; 1.
DR   Gene3D; 1.10.260.40; -; 1.
DR   InterPro; IPR000843; HTH_LacI.
DR   InterPro; IPR010982; Lambda_DNA-bd_dom_sf.
DR   InterPro; IPR001761; Peripla_BP/Lac1_sug-bd_dom.
DR   InterPro; IPR028082; Peripla_BP_I.
DR   Pfam; PF00356; LacI; 1.
DR   Pfam; PF00532; Peripla_BP_1; 1.
DR   SMART; SM00354; HTH_LACI; 1.
DR   SUPFAM; SSF47413; SSF47413; 1.
DR   SUPFAM; SSF53822; SSF53822; 1.
DR   PROSITE; PS50932; HTH_LACI_2; 1.
PE   1: Evidence at protein level;
KW   Direct protein sequencing; DNA-binding; Plasmid; Repressor; Transcription;
KW   Transcription regulation.
FT   INIT_MET        1
FT                   /note="Removed"
FT                   /evidence="ECO:0000269|PubMed:2180920"
FT   CHAIN           2..336
FT                   /note="HTH-type transcriptional regulator RafR"
FT                   /id="PRO_0000107982"
FT   DOMAIN          2..55
FT                   /note="HTH lacI-type"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00111"
FT   DNA_BIND        3..22
FT                   /note="H-T-H motif"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00111"
FT   MUTAGEN         50
FT                   /note="A->E: Loss of activity."
SQ   SEQUENCE   336 AA;  36774 MW;  60D313A0EDA71564 CRC64;
     MSLKAIATTL GISVTTVSRA LGGFSDVAAS TRERVEAEAR RRGYRPNTQA RRLKTGKTDA
     IGLVYPENDV PFNSGVFMDM VSCISRELAY HDIDLLLIAD DEHADCHSYM RLVESRRIDA
     LIIAHTLDDD PRITHLHKAG IPFLALGRVP QGLPCAWFDF DNHAGTWQAT QKLIALGHKS
     IALLSENTSH SYVIARRQGW LDALHEHGLK DPLLRLVSPT RRAGYLAVME LMSLPAPPTA
     IITDNDLSGD GAAMALQLRG RLSGKEAVSL VVYDGLPQDS IIELDVAAVI QSTRSLVGRQ
     ISDMVYQIIN GASPESLQIT WTPIFYPGST VHSPSF