RAF_YEAST
ID RAF_YEAST Reviewed; 181 AA.
AC Q06891; Q06966; Q06977;
DT 03-APR-2007, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1996, sequence version 1.
DT 03-AUG-2022, entry version 68.
DE RecName: Full=Trans-acting factor D {ECO:0000305};
DE AltName: Full=REP-antagonizing factor {ECO:0000303|PubMed:2832156};
DE AltName: Full=Recombinase-activating factor {ECO:0000303|PubMed:2832156};
DE Short=RAF;
GN Name=RAF1 {ECO:0000303|PubMed:2832156};
GN OrderedLocusNames=R0030W {ECO:0000312|SGD:S000029674};
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OG Plasmid 2-micron.
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=A364A D5;
RX PubMed=6251374; DOI=10.1038/286860a0;
RA Hartley J.L., Donelson J.E.;
RT "Nucleotide sequence of the yeast plasmid.";
RL Nature 286:860-864(1980).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 4108, and ATCC 7754;
RX PubMed=1676387; DOI=10.1016/0378-1119(91)90226-2;
RA Xiao W., Pelcher L.E., Rank G.H.;
RT "Sequence diversity of yeast 2 microns RAF gene and its co-evolution with
RT STB and REP1.";
RL Gene 101:75-80(1991).
RN [3]
RP FUNCTION, AND INDUCTION.
RX PubMed=2832156; DOI=10.1002/j.1460-2075.1987.tb02768.x;
RA Murray J.A.H., Scarpa M., Rossi N., Cesareni G.;
RT "Antagonistic controls regulate copy number of the yeast 2 micron
RT plasmid.";
RL EMBO J. 6:4205-4212(1987).
RN [4]
RP FUNCTION.
RX PubMed=28472368; DOI=10.1093/nar/gkx316;
RA Rizvi S.M.A., Prajapati H.K., Nag P., Ghosh S.K.;
RT "The 2-mum plasmid encoded protein Raf1 regulates both stability and copy
RT number of the plasmid by blocking the formation of the Rep1-Rep2 repressor
RT complex.";
RL Nucleic Acids Res. 45:7167-7179(2017).
RN [5]
RP IDENTIFICATION BY MASS SPECTROMETRY.
RX PubMed=29897761; DOI=10.1021/acs.jproteome.8b00032;
RA He C., Jia C., Zhang Y., Xu P.;
RT "Enrichment-based proteogenomics identifies microproteins, missing
RT proteins, and novel smORFs in Saccharomyces cerevisiae.";
RL J. Proteome Res. 17:2335-2344(2018).
CC -!- FUNCTION: Plays a role in 2-micron plasmid partitioning. Antagonizes
CC transcriptional repression of recombinase FLP by REP1-REP2
CC (PubMed:2832156). Regulates both stability and copy number of the
CC plasmid by blocking the formation of the REP1-REP2 repressor complex
CC (PubMed:28472368). {ECO:0000269|PubMed:2832156,
CC ECO:0000269|PubMed:28472368}.
CC -!- INDUCTION: Repressed by the negative regulatory complex REP1-REP2.
CC {ECO:0000269|PubMed:2832156}.
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DR EMBL; J01347; AAB59342.1; -; Genomic_DNA.
DR EMBL; M57897; AAA34955.1; -; Genomic_DNA.
DR EMBL; M57898; AAA34956.1; -; Genomic_DNA.
DR PIR; JQ1027; JQ1027.
DR PIR; S22793; S22793.
DR PIR; S22872; S22872.
DR AlphaFoldDB; Q06891; -.
DR SMR; Q06891; -.
DR DIP; DIP-518N; -.
DR IntAct; Q06891; 4.
DR MaxQB; Q06891; -.
DR PRIDE; Q06891; -.
DR SGD; S000029674; RAF1.
DR PRO; PR:Q06891; -.
DR Proteomes; UP000002311; Plasmid 2-micron.
DR RNAct; Q06891; protein.
DR GO; GO:0005634; C:nucleus; IC:SGD.
DR GO; GO:0006276; P:plasmid maintenance; IMP:SGD.
DR GO; GO:0030541; P:plasmid partitioning; IDA:SGD.
PE 1: Evidence at protein level;
KW Plasmid; Plasmid partition; Reference proteome.
FT CHAIN 1..181
FT /note="Trans-acting factor D"
FT /id="PRO_0000283651"
FT VARIANT 33
FT /note="V -> A (in strain: ATCC 7754)"
FT VARIANT 36
FT /note="K -> R (in strain: ATCC 7754)"
FT VARIANT 46..51
FT /note="RIPRTI -> KVPQTV (in strain: ATCC 7754)"
FT VARIANT 67..68
FT /note="RS -> KF (in strain: ATCC 7754)"
FT VARIANT 104
FT /note="R -> K (in strain: ATCC 7754)"
FT VARIANT 116
FT /note="L -> S (in strain: ATCC 7754)"
FT VARIANT 125
FT /note="D -> N (in strain: ATCC 7754)"
FT VARIANT 136
FT /note="I -> T (in strain: ATCC 7754)"
FT VARIANT 137
FT /note="R -> H (in strain: ATCC 4108)"
FT VARIANT 144
FT /note="N -> D (in strain: ATCC 7754)"
FT VARIANT 152..157
FT /note="IYDRTC -> NYGRTG (in strain: ATCC 7754)"
FT VARIANT 162
FT /note="N -> D (in strain: ATCC 7754)"
FT VARIANT 171
FT /note="F -> L (in strain: ATCC 7754)"
FT VARIANT 180
FT /note="P -> S (in strain: ATCC 7754)"
SQ SEQUENCE 181 AA; 21290 MW; 260CF848B0A51544 CRC64;
MPYKTAIDCI EELATQCFLS KLTDDDVSTF RRVCSKENDI IKLALRIPRT IDYTSILRLL
YDTLPLRSLS FNEALPLFCY SIDPAQQRQC DLRFYLRDVV KLARPRKRLE MQKALLQWLP
SLLSDVTLQL LNDIRIRFEE IQPNIRQTVL QIYDRTCYPS LNFEHPNLGV FPETDSIFEP
V
