RAG1_ORYSJ
ID RAG1_ORYSJ Reviewed; 163 AA.
AC Q01883; Q01884; Q0D7S5; Q8H4M2;
DT 01-JUN-1994, integrated into UniProtKB/Swiss-Prot.
DT 05-SEP-2006, sequence version 2.
DT 03-AUG-2022, entry version 122.
DE RecName: Full=Seed allergenic protein RAG1;
DE AltName: Full=Seed allergenic protein RA17;
DE AltName: Allergen=Ory s aA_TI;
DE Flags: Precursor;
GN Name=RAG1; Synonyms=RA17; OrderedLocusNames=Os07g0214100, LOC_Os07g11360;
GN ORFNames=OJ1116_C08.112;
OS Oryza sativa subsp. japonica (Rice).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX NCBI_TaxID=39947;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC TISSUE=Seed;
RX PubMed=1376283; DOI=10.1016/0014-5793(92)80443-k;
RA Izumi H., Adachi T., Fujii N., Matsuda T., Nakamura R., Tanaka K.,
RA Urisu A., Kurosawa Y.;
RT "Nucleotide sequence of a cDNA clone encoding a major allergenic protein in
RT rice seeds. Homology of the deduced amino acid sequence with members of
RT alpha-amylase/trypsin inhibitor family.";
RL FEBS Lett. 302:213-216(1992).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
RC STRAIN=cv. Nipponbare; TISSUE=Seed;
RX PubMed=7678765; DOI=10.1007/bf00019940;
RA Adachi T., Izumi H., Yamada T., Tanaka K., Takeuchi S., Nakamura R.,
RA Matsuda T.;
RT "Gene structure and expression of rice seed allergenic proteins belonging
RT to the alpha-amylase/trypsin inhibitor family.";
RL Plant Mol. Biol. 21:239-248(1993).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16100779; DOI=10.1038/nature03895;
RG International rice genome sequencing project (IRGSP);
RT "The map-based sequence of the rice genome.";
RL Nature 436:793-800(2005).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=18089549; DOI=10.1093/nar/gkm978;
RG The rice annotation project (RAP);
RT "The rice annotation project database (RAP-DB): 2008 update.";
RL Nucleic Acids Res. 36:D1028-D1033(2008).
RN [5]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT "Improvement of the Oryza sativa Nipponbare reference genome using next
RT generation sequence and optical map data.";
RL Rice 6:4-4(2013).
RN [6]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Nipponbare;
RG The rice full-length cDNA consortium;
RT "Oryza sativa full length cDNA.";
RL Submitted (OCT-2006) to the EMBL/GenBank/DDBJ databases.
RN [7]
RP DISULFIDE BONDS, AND ALLERGEN.
RX PubMed=10664838; DOI=10.1271/bbb.63.2059;
RA Izumi H., Sugiyama M., Matsuda T., Nakamura R.;
RT "Structural characterization of the 16-kDa allergen, RA17, in rice seeds.
RT Prediction of the secondary structure and identification of intramolecular
RT disulfide bridges.";
RL Biosci. Biotechnol. Biochem. 63:2059-2063(1999).
CC -!- FUNCTION: Seed storage protein. {ECO:0000305}.
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000305}.
CC -!- PTM: Five disulfide bonds are present. {ECO:0000269|PubMed:10664838}.
CC -!- ALLERGEN: Causes an allergic reaction in human. Binds to IgE.
CC {ECO:0000305|PubMed:10664838}.
CC -!- SIMILARITY: Belongs to the cereal trypsin/alpha-amylase inhibitor
CC family. {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; X66257; CAA46983.1; -; mRNA.
DR EMBL; D11431; BAA01997.1; -; mRNA.
DR EMBL; D11433; BAA01999.1; -; Genomic_DNA.
DR EMBL; AP004002; BAC20652.1; -; Genomic_DNA.
DR EMBL; AP008213; BAF21098.1; -; Genomic_DNA.
DR EMBL; AP014963; BAT00617.1; -; Genomic_DNA.
DR EMBL; AK242324; BAH01261.1; -; mRNA.
DR PIR; S21157; S21157.
DR PIR; S31081; S31081.
DR RefSeq; XP_015646665.1; XM_015791179.1.
DR AlphaFoldDB; Q01883; -.
DR SMR; Q01883; -.
DR STRING; 4530.OS07T0214100-01; -.
DR Allergome; 1049; Ory s aA_TI.
DR PaxDb; Q01883; -.
DR PRIDE; Q01883; -.
DR EnsemblPlants; Os07t0214100-01; Os07t0214100-01; Os07g0214100.
DR GeneID; 4342722; -.
DR Gramene; Os07t0214100-01; Os07t0214100-01; Os07g0214100.
DR KEGG; osa:4342722; -.
DR eggNOG; ENOG502R43K; Eukaryota.
DR HOGENOM; CLU_140628_0_0_1; -.
DR InParanoid; Q01883; -.
DR OMA; MADHHQV; -.
DR OrthoDB; 1613213at2759; -.
DR Proteomes; UP000000763; Chromosome 7.
DR Proteomes; UP000059680; Chromosome 7.
DR Genevisible; Q01883; OS.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR GO; GO:0019863; F:IgE binding; IDA:UniProtKB.
DR GO; GO:0045735; F:nutrient reservoir activity; IEA:UniProtKB-KW.
DR GO; GO:0004867; F:serine-type endopeptidase inhibitor activity; ISS:Gramene.
DR CDD; cd00261; AAI_SS; 1.
DR Gene3D; 1.10.110.10; -; 1.
DR InterPro; IPR044723; AAI_SS_dom.
DR InterPro; IPR002411; Allergen/amylase_inhib_rice.
DR InterPro; IPR006106; Allergen/soft/tryp_amyl_inhib.
DR InterPro; IPR006105; Allergen/tryp_amyl_inhib_CS.
DR InterPro; IPR036312; Bifun_inhib/LTP/seed_sf.
DR InterPro; IPR016140; Bifunc_inhib/LTP/seed_store.
DR Pfam; PF00234; Tryp_alpha_amyl; 1.
DR PIRSF; PIRSF001657; Allergen/amylase_inhib; 1.
DR PRINTS; PR00808; AMLASEINHBTR.
DR PRINTS; PR00809; RAGALLERGEN.
DR SMART; SM00499; AAI; 1.
DR SUPFAM; SSF47699; SSF47699; 1.
DR PROSITE; PS00426; CEREAL_TRYP_AMYL_INH; 1.
PE 1: Evidence at protein level;
KW Allergen; Disulfide bond; Reference proteome; Secreted;
KW Seed storage protein; Signal; Storage protein.
FT SIGNAL 1..27
FT /evidence="ECO:0000255"
FT CHAIN 28..163
FT /note="Seed allergenic protein RAG1"
FT /id="PRO_0000014361"
FT DISULFID 39..90
FT /note="Or C-39 with C-92"
FT /evidence="ECO:0000269|PubMed:10664838"
FT DISULFID 53..78
FT /note="Or C-53 with C-79"
FT /evidence="ECO:0000269|PubMed:10664838"
FT DISULFID 61..122
FT /evidence="ECO:0000269|PubMed:10664838"
FT DISULFID 79..138
FT /note="Or C-78 with C-138"
FT /evidence="ECO:0000269|PubMed:10664838"
FT DISULFID 92..150
FT /note="Or C-90 with C-150"
FT /evidence="ECO:0000269|PubMed:10664838"
FT CONFLICT 67
FT /note="Missing (in Ref. 1; CAA46983 and 2; BAA01997/
FT BAA01999)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 163 AA; 17568 MW; B39905ACC682A839 CRC64;
MASNKVVFSV LLLVVLSVLA AAMATMADHH QVYSPGEQCR PGISYPTYSL PQCRTLVRRQ
CVGRGAASAA DEQVWQDCCR QLAAVDDGWC RCGALDHMLS GIYRELGATE AGHPMAEVFP
GCRRGDLERA AASLPAFCNV DIPNGPGGVC YWLGYPRTPR TGH
