RAG2_ORYSJ
ID RAG2_ORYSJ Reviewed; 166 AA.
AC Q01882; Q01885; Q0D7S4; Q40652; Q7F174; Q9ST74;
DT 01-JUN-1994, integrated into UniProtKB/Swiss-Prot.
DT 05-SEP-2006, sequence version 2.
DT 03-AUG-2022, entry version 118.
DE RecName: Full=Seed allergenic protein RAG2;
DE AltName: Full=Seed allergenic protein RA14;
DE AltName: Allergen=Ory s aA_TI;
DE Flags: Precursor;
GN Name=RAG2; Synonyms=RA14; OrderedLocusNames=Os07g0214300, LOC_Os07g11380;
GN ORFNames=OJ1116_C08.114, OsJ_23551 {ECO:0000312|EMBL:EAZ39123.1};
OS Oryza sativa subsp. japonica (Rice).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX NCBI_TaxID=39947;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
RC STRAIN=cv. Nipponbare; TISSUE=Seed;
RX PubMed=7678765; DOI=10.1007/bf00019940;
RA Adachi T., Izumi H., Yamada T., Tanaka K., Takeuchi S., Nakamura R.,
RA Matsuda T.;
RT "Gene structure and expression of rice seed allergenic proteins belonging
RT to the alpha-amylase/trypsin inhibitor family.";
RL Plant Mol. Biol. 21:239-248(1993).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=cv. Nipponbare; TISSUE=Endosperm;
RX PubMed=7669811; DOI=10.1016/0167-4838(95)00075-6;
RA Alvarez A.M., Adachi T., Nakase M., Aoki N., Nakamura R., Matsuda T.;
RT "Classification of rice allergenic protein cDNAs belonging to the alpha-
RT amylase/trypsin inhibitor gene family.";
RL Biochim. Biophys. Acta 1251:201-204(1995).
RN [3]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC TISSUE=Endosperm;
RA Wen L., Pan J., Huang J.K., Johnson B.H., Wang C.S., Chen M.S.,
RA Muthukrishnon S., Reeck G.R.;
RT "A cDNA clone that encodes rice low molecular weight globulin.";
RL Submitted (SEP-1991) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=cv. Ilpoombyeo; TISSUE=Seed;
RA Yoon U.H., Kim Y.H.;
RT "Molecular cloning of allergen RAG2 genes in rice seeds.";
RL Submitted (NOV-2006) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16100779; DOI=10.1038/nature03895;
RG International rice genome sequencing project (IRGSP);
RT "The map-based sequence of the rice genome.";
RL Nature 436:793-800(2005).
RN [6]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=18089549; DOI=10.1093/nar/gkm978;
RG The rice annotation project (RAP);
RT "The rice annotation project database (RAP-DB): 2008 update.";
RL Nucleic Acids Res. 36:D1028-D1033(2008).
RN [7]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT "Improvement of the Oryza sativa Nipponbare reference genome using next
RT generation sequence and optical map data.";
RL Rice 6:4-4(2013).
RN [8]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=15685292; DOI=10.1371/journal.pbio.0030038;
RA Yu J., Wang J., Lin W., Li S., Li H., Zhou J., Ni P., Dong W., Hu S.,
RA Zeng C., Zhang J., Zhang Y., Li R., Xu Z., Li S., Li X., Zheng H., Cong L.,
RA Lin L., Yin J., Geng J., Li G., Shi J., Liu J., Lv H., Li J., Wang J.,
RA Deng Y., Ran L., Shi X., Wang X., Wu Q., Li C., Ren X., Wang J., Wang X.,
RA Li D., Liu D., Zhang X., Ji Z., Zhao W., Sun Y., Zhang Z., Bao J., Han Y.,
RA Dong L., Ji J., Chen P., Wu S., Liu J., Xiao Y., Bu D., Tan J., Yang L.,
RA Ye C., Zhang J., Xu J., Zhou Y., Yu Y., Zhang B., Zhuang S., Wei H.,
RA Liu B., Lei M., Yu H., Li Y., Xu H., Wei S., He X., Fang L., Zhang Z.,
RA Zhang Y., Huang X., Su Z., Tong W., Li J., Tong Z., Li S., Ye J., Wang L.,
RA Fang L., Lei T., Chen C.-S., Chen H.-C., Xu Z., Li H., Huang H., Zhang F.,
RA Xu H., Li N., Zhao C., Li S., Dong L., Huang Y., Li L., Xi Y., Qi Q.,
RA Li W., Zhang B., Hu W., Zhang Y., Tian X., Jiao Y., Liang X., Jin J.,
RA Gao L., Zheng W., Hao B., Liu S.-M., Wang W., Yuan L., Cao M.,
RA McDermott J., Samudrala R., Wang J., Wong G.K.-S., Yang H.;
RT "The genomes of Oryza sativa: a history of duplications.";
RL PLoS Biol. 3:266-281(2005).
RN [9]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=12869764; DOI=10.1126/science.1081288;
RG The rice full-length cDNA consortium;
RT "Collection, mapping, and annotation of over 28,000 cDNA clones from
RT japonica rice.";
RL Science 301:376-379(2003).
RN [10]
RP IDENTIFICATION BY MASS SPECTROMETRY, AND ALLERGEN.
RX PubMed=21300107; DOI=10.1016/j.yrtph.2011.01.008;
RA Satoh R., Nakamura R., Komatsu A., Oshima M., Teshima R.;
RT "Proteomic analysis of known and candidate rice allergens between non-
RT transgenic and transgenic plants.";
RL Regul. Toxicol. Pharmacol. 59:437-444(2011).
CC -!- FUNCTION: Seed storage protein. {ECO:0000305}.
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000305}.
CC -!- PTM: Five disulfide bonds are present. {ECO:0000250|UniProtKB:Q01883}.
CC -!- ALLERGEN: Causes an allergic reaction in human. Binds to IgE.
CC {ECO:0000269|PubMed:21300107}.
CC -!- SIMILARITY: Belongs to the cereal trypsin/alpha-amylase inhibitor
CC family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=BAA07710.1; Type=Frameshift; Evidence={ECO:0000305};
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DR EMBL; D11432; BAA01998.1; -; mRNA.
DR EMBL; D11434; BAA02000.1; -; Genomic_DNA.
DR EMBL; D42139; BAA07710.1; ALT_FRAME; mRNA.
DR EMBL; EF122493; ABL74580.1; -; mRNA.
DR EMBL; X62091; CAA44001.1; -; mRNA.
DR EMBL; AP004002; BAC20654.1; -; Genomic_DNA.
DR EMBL; AP008213; BAF21099.1; -; Genomic_DNA.
DR EMBL; AP014963; BAT00618.1; -; Genomic_DNA.
DR EMBL; CM000144; EAZ39123.1; -; Genomic_DNA.
DR EMBL; AK107328; -; NOT_ANNOTATED_CDS; mRNA.
DR PIR; S31080; S31080.
DR PIR; S31082; S31082.
DR PIR; S59922; S59922.
DR AlphaFoldDB; Q01882; -.
DR SMR; Q01882; -.
DR STRING; 4530.OS07T0214300-01; -.
DR Allergome; 1049; Ory s aA_TI.
DR PaxDb; Q01882; -.
DR PRIDE; Q01882; -.
DR EnsemblPlants; Os07t0214300-01; Os07t0214300-01; Os07g0214300.
DR Gramene; Os07t0214300-01; Os07t0214300-01; Os07g0214300.
DR eggNOG; ENOG502R43K; Eukaryota.
DR HOGENOM; CLU_140628_0_0_1; -.
DR InParanoid; Q01882; -.
DR OMA; DEWCICG; -.
DR Proteomes; UP000000763; Chromosome 7.
DR Proteomes; UP000007752; Chromosome 7.
DR Proteomes; UP000059680; Chromosome 7.
DR Genevisible; Q01882; OS.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR GO; GO:0019863; F:IgE binding; IDA:UniProtKB.
DR GO; GO:0004867; F:serine-type endopeptidase inhibitor activity; ISS:Gramene.
DR CDD; cd00261; AAI_SS; 1.
DR Gene3D; 1.10.110.10; -; 1.
DR InterPro; IPR044723; AAI_SS_dom.
DR InterPro; IPR002411; Allergen/amylase_inhib_rice.
DR InterPro; IPR006106; Allergen/soft/tryp_amyl_inhib.
DR InterPro; IPR006105; Allergen/tryp_amyl_inhib_CS.
DR InterPro; IPR036312; Bifun_inhib/LTP/seed_sf.
DR InterPro; IPR016140; Bifunc_inhib/LTP/seed_store.
DR Pfam; PF00234; Tryp_alpha_amyl; 1.
DR PIRSF; PIRSF001657; Allergen/amylase_inhib; 1.
DR PRINTS; PR00808; AMLASEINHBTR.
DR PRINTS; PR00809; RAGALLERGEN.
DR SMART; SM00499; AAI; 1.
DR SUPFAM; SSF47699; SSF47699; 1.
DR PROSITE; PS00426; CEREAL_TRYP_AMYL_INH; 1.
PE 1: Evidence at protein level;
KW Allergen; Disulfide bond; Glycoprotein; Reference proteome; Secreted;
KW Signal.
FT SIGNAL 1..26
FT /evidence="ECO:0000255"
FT CHAIN 27..166
FT /note="Seed allergenic protein RAG2"
FT /id="PRO_0000014360"
FT CARBOHYD 147
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT DISULFID 41..93
FT /note="Or C-41 with C-95"
FT /evidence="ECO:0000250|UniProtKB:Q01883"
FT DISULFID 55..81
FT /note="Or C-55 with C-82"
FT /evidence="ECO:0000250|UniProtKB:Q01883"
FT DISULFID 63..125
FT /evidence="ECO:0000250|UniProtKB:Q01883"
FT DISULFID 82..141
FT /note="Or C-81 with C-141"
FT /evidence="ECO:0000250|UniProtKB:Q01883"
FT DISULFID 95..153
FT /note="Or C-93 with C-153"
FT /evidence="ECO:0000250|UniProtKB:Q01883"
FT CONFLICT 20..23
FT /note="AATA -> RRDG (in Ref. 3; CAA44001)"
FT /evidence="ECO:0000305"
FT CONFLICT 23..24
FT /note="AT -> TR (in Ref. 1; BAA01998 and 2; BAA07710)"
FT /evidence="ECO:0000305"
FT CONFLICT 66..68
FT /note="HGA -> TRS (in Ref. 1; BAA01998)"
FT /evidence="ECO:0000305"
FT CONFLICT 71
FT /note="Missing (in Ref. 1; BAA01998 and 3; CAA44001)"
FT /evidence="ECO:0000305"
FT CONFLICT 78
FT /note="R -> A (in Ref. 1; BAA01998)"
FT /evidence="ECO:0000305"
FT CONFLICT 84
FT /note="Q -> E (in Ref. 1; BAA01998)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 166 AA; 17865 MW; 7CE939A6A6125956 CRC64;
MASNKVVFSA LLLIIVSVLA ATATMADHHK DQVVYSLGER CQPGMGYPMY SLPRCRAVVK
RQCVGHGAPG GAVDEQLRQD CCRQLAAVDD SWCRCSALNH MVGGIYRELG ATDVGHPMAE
VFPGCRRGDL ERAAASLPAF CNVDIPNGTG GVCYWLGYPR TPRTGH
