RAM1_LOTJA
ID RAM1_LOTJA Reviewed; 693 AA.
AC A0A145P7T2;
DT 17-JUN-2020, integrated into UniProtKB/Swiss-Prot.
DT 08-JUN-2016, sequence version 1.
DT 25-MAY-2022, entry version 24.
DE RecName: Full=GRAS family protein RAM1 {ECO:0000305};
DE AltName: Full=Protein REDUCED AND DEGENERATE ARBUSCULES {ECO:0000303|PubMed:27020747};
DE AltName: Full=Protein REQUIRED FOR ARBUSCULAR MYCORRHIZATION 1 {ECO:0000303|PubMed:25560877, ECO:0000303|PubMed:27020747};
DE Short=LjRAM1 {ECO:0000303|PubMed:25560877, ECO:0000303|PubMed:27020747};
GN Name=RAM1 {ECO:0000303|PubMed:25560877, ECO:0000303|PubMed:27020747};
GN Synonyms=RED {ECO:0000303|PubMed:27020747};
OS Lotus japonicus (Lotus corniculatus var. japonicus).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade;
OC NPAAA clade; Hologalegina; robinioid clade; Loteae; Lotus.
OX NCBI_TaxID=34305;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, DISRUPTION PHENOTYPE,
RP INDUCTION BY RHIZOPHAGUS IRREGULARIS, INTERACTION WITH RAD1, SUBUNIT,
RP SUBCELLULAR LOCATION, TISSUE SPECIFICITY, AND DEVELOPMENTAL STAGE.
RC STRAIN=cv. Gifu / B-129;
RX PubMed=25560877; DOI=10.1104/pp.114.255430;
RA Xue L., Cui H., Buer B., Vijayakumar V., Delaux P.-M., Junkermann S.,
RA Bucher M.;
RT "Network of GRAS transcription factors involved in the control of arbuscule
RT development in Lotus japonicus.";
RL Plant Physiol. 167:854-871(2015).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, DISRUPTION PHENOTYPE, TISSUE
RP SPECIFICITY, AND DEVELOPMENTAL STAGE.
RC STRAIN=cv. Gifu;
RX PubMed=27020747; DOI=10.1016/j.cub.2016.01.069;
RA Pimprikar P., Carbonnel S., Paries M., Katzer K., Klingl V., Bohmer M.J.,
RA Karl L., Floss D.S., Harrison M.J., Parniske M., Gutjahr C.;
RT "A CCaMK-CYCLOPS-DELLA complex activates transcription of RAM1 to regulate
RT arbuscule branching.";
RL Curr. Biol. 26:987-998(2016).
CC -!- FUNCTION: Transcription factor acting as a central regulator of
CC arbuscular mycorrhiza (AM)-related genes (e.g. PT4) required for the
CC morphogenesis of arbuscules upon symbiosis with AM fungi (e.g.
CC Rhizophagus irregularis) (PubMed:25560877, PubMed:27020747). Also
CC involved in restricting mycorrhizal colonization of the root meristem
CC (By similarity). {ECO:0000250|UniProtKB:A0A0M4FMK2,
CC ECO:0000269|PubMed:25560877, ECO:0000269|PubMed:27020747}.
CC -!- SUBUNIT: Forms homodimer in nucleus and cytoplasm (PubMed:25560877).
CC Interacts with RAD1 (PubMed:25560877). Interacts with NSP2 and TF80 (By
CC similarity). {ECO:0000250|UniProtKB:G7L166,
CC ECO:0000269|PubMed:25560877}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:25560877}. Cytoplasm
CC {ECO:0000269|PubMed:25560877}.
CC -!- TISSUE SPECIFICITY: Present in roots and leaves (PubMed:25560877).
CC Accumulates specifically in arbuscular mycorrhiza fungi colonized roots
CC (PubMed:27020747). {ECO:0000269|PubMed:25560877,
CC ECO:0000269|PubMed:27020747}.
CC -!- DEVELOPMENTAL STAGE: During arbuscular mycorrhizal (AM) symbiosis,
CC accumulates in root cortical cells, root tips and lateral root
CC primordia, restricted to colonized root segments.
CC {ECO:0000269|PubMed:25560877, ECO:0000269|PubMed:27020747}.
CC -!- INDUCTION: Induced upon root colonization by arbuscular mycorrhiza (AM)
CC fungi (e.g. Rhizophagus irregularis) in roots and root tips
CC (PubMed:25560877). Regulated by a complex comprising CCaMK, CYCLOPS,
CC and DELLA during AM symbiosis (PubMed:27020747).
CC {ECO:0000269|PubMed:25560877, ECO:0000269|PubMed:27020747}.
CC -!- DISRUPTION PHENOTYPE: Ineffective symbiosis with arbuscular mycorrhiza
CC (AM) fungi (e.g. Rhizophagus irregularis) with abnormal AM-related
CC genes expression, leading to rare colonizations characterized by
CC malformed arbuscules. {ECO:0000269|PubMed:25560877,
CC ECO:0000269|PubMed:27020747}.
CC -!- SIMILARITY: Belongs to the GRAS family. {ECO:0000255|PROSITE-
CC ProRule:PRU01191}.
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DR EMBL; KU500627; ANP22370.1; -; Genomic_DNA.
DR EMBL; KU557503; AMV91704.1; -; Transcribed_RNA.
DR AlphaFoldDB; A0A145P7T2; -.
DR SMR; A0A145P7T2; -.
DR GO; GO:0005737; C:cytoplasm; IDA:UniProtKB.
DR GO; GO:0005634; C:nucleus; IDA:UniProtKB.
DR GO; GO:0042803; F:protein homodimerization activity; IDA:UniProtKB.
DR GO; GO:0036377; P:arbuscular mycorrhizal association; IMP:UniProtKB.
DR GO; GO:0006355; P:regulation of transcription, DNA-templated; IMP:UniProtKB.
DR GO; GO:0009610; P:response to symbiotic fungus; IEP:UniProtKB.
DR InterPro; IPR005202; TF_GRAS.
DR PANTHER; PTHR31636; PTHR31636; 1.
DR Pfam; PF03514; GRAS; 1.
DR PROSITE; PS50985; GRAS; 1.
PE 1: Evidence at protein level;
KW Cytoplasm; Nucleus; Transcription; Transcription regulation.
FT CHAIN 1..693
FT /note="GRAS family protein RAM1"
FT /id="PRO_0000450023"
FT DOMAIN 309..692
FT /note="GRAS"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01191"
FT REGION 1..43
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 233..253
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 268..298
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 316..391
FT /note="Leucine repeat I (LRI)"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01191"
FT REGION 378..397
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 410..475
FT /note="VHIID"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01191"
FT REGION 485..516
FT /note="Leucine repeat II (LRII)"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01191"
FT REGION 526..614
FT /note="PFYRE"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01191"
FT REGION 617..692
FT /note="SAW"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01191"
FT MOTIF 323..327
FT /note="LxCxE motif"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01191"
FT MOTIF 441..445
FT /note="VHIID"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01191"
SQ SEQUENCE 693 AA; 76681 MW; 4279B10BF9FB936B CRC64;
MINSMCGSSV SLKSENSRNK PQPTSPNESV LQSKKNATQS SADLEQTSLN LTPPSLNLPA
LKFDLDGDVE VQSPDSSMWE SFFSDHLLDG DFMISSPVRN NVPSPQASTF NSNYNYAHQG
IQSQSLSGCS PPRFSSPLGA FNSNKGKGLS PLHRVFNSPN NQYMQHVENL ALPAIEEFLE
EYQGDHGLGG GGGYSNSSNK VSSDIGSSSE CFDMQNHIPS MLDSLTMQNS SRYCGSVSED
SSVHGGSSQL SQDSDFYHQM GSMASASLSQ ALQQERYQEK QQKQHQTQQQ QQPQQQQQNL
TVPIPIGMDQ EQDSGLQLVH LLLACAEAVA KEEYMLARRY LHHLNRVVTP LGDSMQRVAA
CFTESLSARL AATLTTKPQS ISNGTSMPRS SSSSCLSPFP SNSIEVLKIY QIVYQACPYV
KFAHFTANQA IFEAFEAEER VHVIDLDILQ GYQWPTFMQA LAARPGGAPF LRITGVGPCI
DSVRETGRCL TELAHSLRIP FEFHPVGEQL EDLKPHMFNR RVGEALAVNT VNRLHRVPGS
HLGNLLSMIR DQAPNIVTLV EQEASHNGPY FLGRFLEALH YYSAIFDSLD ATFPPESAQR
AKVEQYIFAP EIRNIVACEG AERIERHERL EKWRKIMEGK GFRGVALSPN AVTQSRILLG
LYSCDGYRLT EDKGCLLLGW QDRAIIAASA WRC
