RAM1_MEDTR
ID RAM1_MEDTR Reviewed; 674 AA.
AC G7L166;
DT 08-MAY-2019, integrated into UniProtKB/Swiss-Prot.
DT 25-JAN-2012, sequence version 1.
DT 25-MAY-2022, entry version 50.
DE RecName: Full=GRAS family protein RAM1 {ECO:0000305};
DE AltName: Full=Protein REQUIRED FOR ARBUSCULAR MYCORRHIZATION 1 {ECO:0000303|PubMed:23122845};
DE Short=MtRAM1 {ECO:0000303|PubMed:23122845};
GN Name=RAM1 {ECO:0000303|PubMed:23122845};
GN OrderedLocusNames=MTR_7g027190 {ECO:0000312|EMBL:AES78313.1};
GN ORFNames=MtrunA17_Chr7g0224271 {ECO:0000312|EMBL:RHN44888.1};
OS Medicago truncatula (Barrel medic) (Medicago tribuloides).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade;
OC NPAAA clade; Hologalegina; IRL clade; Trifolieae; Medicago.
OX NCBI_TaxID=3880;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION, INDUCTION, AND
RP DISRUPTION PHENOTYPE.
RX PubMed=23122845; DOI=10.1016/j.cub.2012.09.044;
RA Gobbato E., Marsh J.F., Vernie T., Wang E., Maillet F., Kim J.,
RA Miller J.B., Sun J., Bano S.A., Ratet P., Mysore K.S., Denarie J.,
RA Schultze M., Oldroyd G.E.;
RT "A GRAS-type transcription factor with a specific function in mycorrhizal
RT signaling.";
RL Curr. Biol. 22:2236-2241(2012).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Jemalong A17;
RX PubMed=22089132; DOI=10.1038/nature10625;
RA Young N.D., Debelle F., Oldroyd G.E.D., Geurts R., Cannon S.B.,
RA Udvardi M.K., Benedito V.A., Mayer K.F.X., Gouzy J., Schoof H.,
RA Van de Peer Y., Proost S., Cook D.R., Meyers B.C., Spannagl M., Cheung F.,
RA De Mita S., Krishnakumar V., Gundlach H., Zhou S., Mudge J., Bharti A.K.,
RA Murray J.D., Naoumkina M.A., Rosen B., Silverstein K.A.T., Tang H.,
RA Rombauts S., Zhao P.X., Zhou P., Barbe V., Bardou P., Bechner M.,
RA Bellec A., Berger A., Berges H., Bidwell S., Bisseling T., Choisne N.,
RA Couloux A., Denny R., Deshpande S., Dai X., Doyle J.J., Dudez A.-M.,
RA Farmer A.D., Fouteau S., Franken C., Gibelin C., Gish J., Goldstein S.,
RA Gonzalez A.J., Green P.J., Hallab A., Hartog M., Hua A., Humphray S.J.,
RA Jeong D.-H., Jing Y., Jocker A., Kenton S.M., Kim D.-J., Klee K., Lai H.,
RA Lang C., Lin S., Macmil S.L., Magdelenat G., Matthews L., McCorrison J.,
RA Monaghan E.L., Mun J.-H., Najar F.Z., Nicholson C., Noirot C.,
RA O'Bleness M., Paule C.R., Poulain J., Prion F., Qin B., Qu C., Retzel E.F.,
RA Riddle C., Sallet E., Samain S., Samson N., Sanders I., Saurat O.,
RA Scarpelli C., Schiex T., Segurens B., Severin A.J., Sherrier D.J., Shi R.,
RA Sims S., Singer S.R., Sinharoy S., Sterck L., Viollet A., Wang B.-B.,
RA Wang K., Wang M., Wang X., Warfsmann J., Weissenbach J., White D.D.,
RA White J.D., Wiley G.B., Wincker P., Xing Y., Yang L., Yao Z., Ying F.,
RA Zhai J., Zhou L., Zuber A., Denarie J., Dixon R.A., May G.D.,
RA Schwartz D.C., Rogers J., Quetier F., Town C.D., Roe B.A.;
RT "The Medicago genome provides insight into the evolution of rhizobial
RT symbioses.";
RL Nature 480:520-524(2011).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Jemalong A17;
RX PubMed=24767513; DOI=10.1186/1471-2164-15-312;
RA Tang H., Krishnakumar V., Bidwell S., Rosen B., Chan A., Zhou S.,
RA Gentzbittel L., Childs K.L., Yandell M., Gundlach H., Mayer K.F.,
RA Schwartz D.C., Town C.D.;
RT "An improved genome release (version Mt4.0) for the model legume Medicago
RT truncatula.";
RL BMC Genomics 15:312-312(2014).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Jemalong A17;
RX PubMed=30397259; DOI=10.1038/s41477-018-0286-7;
RA Pecrix Y., Staton S.E., Sallet E., Lelandais-Briere C., Moreau S.,
RA Carrere S., Blein T., Jardinaud M.F., Latrasse D., Zouine M., Zahm M.,
RA Kreplak J., Mayjonade B., Satge C., Perez M., Cauet S., Marande W.,
RA Chantry-Darmon C., Lopez-Roques C., Bouchez O., Berard A., Debelle F.,
RA Munos S., Bendahmane A., Berges H., Niebel A., Buitink J., Frugier F.,
RA Benhamed M., Crespi M., Gouzy J., Gamas P.;
RT "Whole-genome landscape of Medicago truncatula symbiotic genes.";
RL Nat. Plants 4:1017-1025(2018).
RN [5]
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RC STRAIN=cv. W138;
RX PubMed=25971550; DOI=10.1104/pp.15.00310;
RA Rich M.K., Schorderet M., Bapaume L., Falquet L., Morel P.,
RA Vandenbussche M., Reinhardt D.;
RT "The Petunia GRAS transcription factor ATA/RAM1 regulates symbiotic gene
RT expression and fungal morphogenesis in arbuscular mycorrhiza.";
RL Plant Physiol. 168:788-797(2015).
RN [6]
RP FUNCTION, DISRUPTION PHENOTYPE, INDUCTION BY GLOMUS VERSIFORME AND DELLA1,
RP DEVELOPMENTAL STAGE, AND INTERACTION WITH NSP2; RAD1 AND TF80.
RX PubMed=26511916; DOI=10.1104/pp.15.01155;
RA Park H.-J., Floss D.S., Levesque-Tremblay V., Bravo A., Harrison M.J.;
RT "Hyphal branching during arbuscule development requires reduced arbuscular
RT mycorrhiza1.";
RL Plant Physiol. 169:2774-2788(2015).
CC -!- FUNCTION: Transcription factor acting as a central regulator of
CC arbuscular mycorrhiza (AM)-related genes (e.g. AMT2;4, AMT2;5, EXO70I,
CC STR, RAM2, LEC5, PT4, VPY, BCP1, SCP1 and RAD1) required for the
CC morphogenesis of arbuscules upon symbiosis with AM fungi (e.g.
CC Rhizophagus irregularis and Glomus versiforme) (PubMed:25971550,
CC PubMed:26511916, PubMed:23122845). Promotes directly the expression of
CC RAM2, EXO70I, STR and RAD1 (PubMed:26511916). Not necessary to enable
CC hyphopodium formation or hyphal entry into the root but essential to
CC support arbuscule branching (PubMed:26511916). Involved in the
CC phosphate-mediated suppression of AM fungi colonization in mycorrhiza
CC (PubMed:26511916). Also involved in restricting mycorrhizal
CC colonization of the root meristem (By similarity). Required for Myc
CC factor signaling from mycorrhizal fungi, but has no function in Nod
CC factor signaling from rhizobial bacteria (PubMed:23122845). Regulates
CC the expression of RAM2, a glycerol-3-phosphate acyl transferase that
CC promotes cutin biosynthesis to enhance mycorrhizal hyphopodia formation
CC (PubMed:23122845). {ECO:0000250|UniProtKB:A0A0M4FMK2,
CC ECO:0000269|PubMed:23122845, ECO:0000269|PubMed:25971550,
CC ECO:0000269|PubMed:26511916}.
CC -!- SUBUNIT: Forms homodimer in nucleus and cytoplasm (By similarity).
CC Interacts with NSP2, RAD1 and TF80 (PubMed:26511916).
CC {ECO:0000250|UniProtKB:A0A145P7T2, ECO:0000269|PubMed:26511916}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:23122845}. Cytoplasm
CC {ECO:0000250|UniProtKB:A0A145P7T2}.
CC -!- DEVELOPMENTAL STAGE: Expressed in the cortex of root colonized regions
CC by arbuscular mycorrhizal fungi (e.g. Glomus versiforme), and, to a
CC lesser extent, in noncolonized lateral roots.
CC {ECO:0000269|PubMed:26511916}.
CC -!- INDUCTION: Induced in roots during colonization by arbuscular
CC mycorrhizal (AM) fungi (e.g. Glomus versiforme) (PubMed:23122845,
CC PubMed:26511916). Regulated by DELLA1 during mycorrhizal symbiosis
CC (PubMed:26511916). {ECO:0000269|PubMed:23122845,
CC ECO:0000269|PubMed:26511916}.
CC -!- DISRUPTION PHENOTYPE: No visible phenotype under normal growth
CC conditions (PubMed:23122845). Unable to be colonized by mycorrhizal
CC fungi, with a defect in hyphopodia formation on the surface of the root
CC (PubMed:23122845). Ineffective symbiosis with arbuscular mycorrhiza
CC (AM) fungi (e.g. Rhizophagus irregularis) leading to rare colonizations
CC characterized by malformed arbuscules reduced to trunk with a few
CC initial thick hyphal branches (PubMed:25971550, PubMed:26511916).
CC Impaired induction of AM-related genes (e.g. AMT2;4, AMT2;5, EXO70I,
CC STR, RAM2, LEC5, PT4, VPY, BCP1, SCP1 and RAD1) in the presence of AM-
CC fungi (PubMed:26511916). Insensitivity to phosphate-mediated regulation
CC of the symbiosis with AM fungi (PubMed:26511916).
CC {ECO:0000269|PubMed:23122845, ECO:0000269|PubMed:25971550,
CC ECO:0000269|PubMed:26511916}.
CC -!- SIMILARITY: Belongs to the GRAS family. {ECO:0000255|PROSITE-
CC ProRule:PRU01191, ECO:0000305}.
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DR EMBL; JN572683; AFK81971.2; -; mRNA.
DR EMBL; CM001223; AES78313.1; -; Genomic_DNA.
DR EMBL; PSQE01000007; RHN44888.1; -; Genomic_DNA.
DR RefSeq; XP_003622095.1; XM_003622047.1.
DR AlphaFoldDB; G7L166; -.
DR SMR; G7L166; -.
DR STRING; 3880.AES78313; -.
DR EnsemblPlants; AES78313; AES78313; MTR_7g027190.
DR GeneID; 11438339; -.
DR Gramene; AES78313; AES78313; MTR_7g027190.
DR KEGG; mtr:MTR_7g027190; -.
DR eggNOG; ENOG502QUV6; Eukaryota.
DR HOGENOM; CLU_404622_0_0_1; -.
DR OMA; NNQFMQV; -.
DR OrthoDB; 333519at2759; -.
DR Proteomes; UP000002051; Chromosome 7.
DR Proteomes; UP000265566; Chromosome 7.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0005634; C:nucleus; IDA:UniProtKB.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; IMP:UniProtKB.
DR GO; GO:0043565; F:sequence-specific DNA binding; IBA:GO_Central.
DR GO; GO:0036377; P:arbuscular mycorrhizal association; IMP:UniProtKB.
DR GO; GO:0010247; P:detection of phosphate ion; IMP:UniProtKB.
DR GO; GO:0006355; P:regulation of transcription, DNA-templated; IMP:UniProtKB.
DR GO; GO:0007584; P:response to nutrient; IMP:UniProtKB.
DR GO; GO:0009610; P:response to symbiotic fungus; IEP:UniProtKB.
DR InterPro; IPR005202; TF_GRAS.
DR PANTHER; PTHR31636; PTHR31636; 1.
DR Pfam; PF03514; GRAS; 1.
DR PROSITE; PS50985; GRAS; 1.
PE 1: Evidence at protein level;
KW Cytoplasm; Nucleus; Reference proteome; Transcription;
KW Transcription regulation.
FT CHAIN 1..674
FT /note="GRAS family protein RAM1"
FT /id="PRO_0000447242"
FT DOMAIN 288..673
FT /note="GRAS"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01191"
FT REGION 295..372
FT /note="Leucine repeat I (LRI)"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01191"
FT REGION 391..456
FT /note="VHIID"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01191"
FT REGION 466..497
FT /note="Leucine repeat II (LRII)"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01191"
FT REGION 507..595
FT /note="PFYRE"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01191"
FT REGION 598..673
FT /note="SAW"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01191"
FT MOTIF 302..306
FT /note="LxCxE motif"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01191"
FT MOTIF 422..426
FT /note="VHIID"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01191"
SQ SEQUENCE 674 AA; 74869 MW; 35608495F170E03A CRC64;
MINSLCGSSN SLKEKCLQPN SSNQTNTHSK KNATNSCGDL EQINVLTPQS LNLPSLKFDL
DGDVEVQSPD SSMWEAFFND HLDNDFMISS PIRNINPNSP QASTYNNCNY NYAQGMQIQS
LSGCSPPRFA SQIGSLNSNN QQKGKGLSPL HRVFNSPNNQ YMQHVENLSL PAIEEFLEDF
QGDVDHFSST KVSSECFDME TPISTILDSL TMQNSSSYGA SVNEESTLLH GGNSSSQISQ
ESDIYHQMGS MASASLSQAL QQERYQEKHQ KMQAQQQSLT VPIQIGIEQE QDSGLQLVHL
LLACAEAVAK GEYMLARRYL HQLNRVVTPL GDSMQRVASC FTESLSARLA ATLTTKSSST
KKLAPSSLSS SSSSSCLSTF PSNPMEVLKI YQIVYQACPY IKFAHFTANQ AIFEAFEAEE
RVHVIDLDIL QGYQWPAFMQ ALAARPGGAP FLRITGVGPC IESVRETGRC LTELAHSLRI
PFEFHPVGEQ LEDLKPHMFN RRVGEALAVN TVNRLHRVPG NHLGNLLSMI RDQAPNIVTL
VEQEASHNGP YFLGRFLEAL HYYSAIFDSL DATFPVESAP RAKVEQYIFA PEIRNIVACE
GEERIERHER LEKWRKIMEG KGFKGVPLSP NAVTQSRILL GLYSCDGYRL TEDKGCLLLG
WQDRAIIAAS AWRC
