RAMA_CORGL
ID RAMA_CORGL Reviewed; 281 AA.
AC Q8NML3; Q6M2S0;
DT 27-MAY-2015, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2002, sequence version 1.
DT 03-AUG-2022, entry version 129.
DE RecName: Full=HTH-type transcriptional activator RamA {ECO:0000303|PubMed:16547043};
DE AltName: Full=LuxR-type transcriptional regulator {ECO:0000303|PubMed:16547043};
DE AltName: Full=Regulator of acetate metabolism A {ECO:0000303|PubMed:16547043};
DE Short=RAMA {ECO:0000303|PubMed:16547043};
GN Name=ramA {ECO:0000303|PubMed:16547043}; OrderedLocusNames=cg2831, Cgl2560;
OS Corynebacterium glutamicum (strain ATCC 13032 / DSM 20300 / BCRC 11384 /
OS JCM 1318 / LMG 3730 / NCIMB 10025).
OC Bacteria; Actinobacteria; Corynebacteriales; Corynebacteriaceae;
OC Corynebacterium.
OX NCBI_TaxID=196627;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Nakagawa S.;
RT "Complete genomic sequence of Corynebacterium glutamicum ATCC 13032.";
RL Submitted (MAY-2002) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 13032 / DSM 20300 / BCRC 11384 / JCM 1318 / LMG 3730 / NCIMB
RC 10025;
RX PubMed=12743753; DOI=10.1007/s00253-003-1328-1;
RA Ikeda M., Nakagawa S.;
RT "The Corynebacterium glutamicum genome: features and impacts on
RT biotechnological processes.";
RL Appl. Microbiol. Biotechnol. 62:99-109(2003).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 13032 / DSM 20300 / BCRC 11384 / JCM 1318 / LMG 3730 / NCIMB
RC 10025;
RX PubMed=12948626; DOI=10.1016/s0168-1656(03)00154-8;
RA Kalinowski J., Bathe B., Bartels D., Bischoff N., Bott M., Burkovski A.,
RA Dusch N., Eggeling L., Eikmanns B.J., Gaigalat L., Goesmann A.,
RA Hartmann M., Huthmacher K., Kraemer R., Linke B., McHardy A.C., Meyer F.,
RA Moeckel B., Pfefferle W., Puehler A., Rey D.A., Rueckert C., Rupp O.,
RA Sahm H., Wendisch V.F., Wiegraebe I., Tauch A.;
RT "The complete Corynebacterium glutamicum ATCC 13032 genome sequence and its
RT impact on the production of L-aspartate-derived amino acids and vitamins.";
RL J. Biotechnol. 104:5-25(2003).
RN [4]
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RC STRAIN=ATCC 13032 / DSM 20300 / BCRC 11384 / JCM 1318 / LMG 3730 / NCIMB
RC 10025;
RX PubMed=16547043; DOI=10.1128/jb.188.7.2554-2567.2006;
RA Cramer A., Gerstmeir R., Schaffer S., Bott M., Eikmanns B.J.;
RT "Identification of RamA, a novel LuxR-type transcriptional regulator of
RT genes involved in acetate metabolism of Corynebacterium glutamicum.";
RL J. Bacteriol. 188:2554-2567(2006).
RN [5]
RP FUNCTION.
RC STRAIN=ATCC 13032 / DSM 20300 / BCRC 11384 / JCM 1318 / LMG 3730 / NCIMB
RC 10025;
RX PubMed=17114251; DOI=10.1128/jb.01061-06;
RA Cramer A., Auchter M., Frunzke J., Bott M., Eikmanns B.J.;
RT "RamB, the transcriptional regulator of acetate metabolism in
RT Corynebacterium glutamicum, is subject to regulation by RamA and RamB.";
RL J. Bacteriol. 189:1145-1149(2007).
RN [6]
RP INDUCTION.
RC STRAIN=ATCC 13032 / DSM 20300 / BCRC 11384 / JCM 1318 / LMG 3730 / NCIMB
RC 10025;
RX PubMed=17183211; DOI=10.1159/000096459;
RA Cramer A., Eikmanns B.J.;
RT "RamA, the transcriptional regulator of acetate metabolism in
RT Corynebacterium glutamicum, is subject to negative autoregulation.";
RL J. Mol. Microbiol. Biotechnol. 12:51-59(2007).
RN [7]
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RC STRAIN=ATCC 13032 / DSM 20300 / BCRC 11384 / JCM 1318 / LMG 3730 / NCIMB
RC 10025;
RX PubMed=18355281; DOI=10.1111/j.1574-6968.2008.01098.x;
RA Jungwirth B., Emer D., Brune I., Hansmeier N., Puehler A., Eikmanns B.J.,
RA Tauch A.;
RT "Triple transcriptional control of the resuscitation promoting factor 2
RT (rpf2) gene of Corynebacterium glutamicum by the regulators of acetate
RT metabolism RamA and RamB and the cAMP-dependent regulator GlxR.";
RL FEMS Microbiol. Lett. 281:190-197(2008).
RN [8]
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RC STRAIN=ATCC 13032 / DSM 20300 / BCRC 11384 / JCM 1318 / LMG 3730 / NCIMB
RC 10025;
RX PubMed=19095019; DOI=10.1016/j.jbiotec.2008.11.003;
RA Emer D., Krug A., Eikmanns B.J., Bott M.;
RT "Complex expression control of the Corynebacterium glutamicum aconitase
RT gene: identification of RamA as a third transcriptional regulator besides
RT AcnR and RipA.";
RL J. Biotechnol. 140:92-98(2009).
CC -!- FUNCTION: RamA is a master regulator of acetate metabolism. It
CC positively controls the expression of acnA, aceA, aceB, ack, pta and
CC ramB genes in the presence of acetate (PubMed:16547043,
CC PubMed:17114251, PubMed:19095019). RamA is also a positive regulator of
CC rpf2 gene expression during growth on glucose as the sole carbon source
CC (PubMed:18355281). {ECO:0000269|PubMed:16547043,
CC ECO:0000269|PubMed:17114251, ECO:0000269|PubMed:18355281,
CC ECO:0000269|PubMed:19095019}.
CC -!- INDUCTION: RamA represses its own expression.
CC {ECO:0000269|PubMed:17183211}.
CC -!- DISRUPTION PHENOTYPE: Cells lacking this gene grow on acetate with the
CC same aconitase activity as cells on glucose. This mutant is unable to
CC grow on acetate as the sole carbon and energy source and, in comparison
CC to the wild-type, shows very low specific activities of
CC phosphotransacetylase, acetate kinase, isocitrate lyase, and malate
CC synthase, irrespective of the presence of acetate in the medium
CC (PubMed:16547043, PubMed:19095019). The transcription level of the rpf2
CC gene is reduced 2.5-fold in the ramA deletion mutant during growth on
CC glucose as the sole carbon source (PubMed:18355281).
CC {ECO:0000269|PubMed:16547043, ECO:0000269|PubMed:18355281,
CC ECO:0000269|PubMed:19095019}.
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DR EMBL; BA000036; BAB99953.1; -; Genomic_DNA.
DR EMBL; BX927155; CAF21222.1; -; Genomic_DNA.
DR RefSeq; NP_601759.1; NC_003450.3.
DR RefSeq; WP_003857068.1; NC_006958.1.
DR AlphaFoldDB; Q8NML3; -.
DR SMR; Q8NML3; -.
DR STRING; 196627.cg2831; -.
DR PRIDE; Q8NML3; -.
DR DNASU; 1020507; -.
DR GeneID; 58311472; -.
DR KEGG; cgb:cg2831; -.
DR KEGG; cgl:Cgl2560; -.
DR PATRIC; fig|196627.13.peg.2495; -.
DR eggNOG; COG2197; Bacteria.
DR HOGENOM; CLU_081562_0_0_11; -.
DR OMA; TMTARCL; -.
DR Proteomes; UP000000582; Chromosome.
DR GO; GO:0003677; F:DNA binding; IDA:UniProtKB.
DR GO; GO:0006083; P:acetate metabolic process; IMP:UniProtKB.
DR GO; GO:0006355; P:regulation of transcription, DNA-templated; IMP:UniProtKB.
DR CDD; cd06170; LuxR_C_like; 1.
DR Gene3D; 1.10.10.10; -; 1.
DR Gene3D; 3.30.450.40; -; 1.
DR InterPro; IPR003018; GAF.
DR InterPro; IPR029016; GAF-like_dom_sf.
DR InterPro; IPR016032; Sig_transdc_resp-reg_C-effctor.
DR InterPro; IPR000792; Tscrpt_reg_LuxR_C.
DR InterPro; IPR036388; WH-like_DNA-bd_sf.
DR Pfam; PF13185; GAF_2; 1.
DR Pfam; PF00196; GerE; 1.
DR PRINTS; PR00038; HTHLUXR.
DR SMART; SM00065; GAF; 1.
DR SMART; SM00421; HTH_LUXR; 1.
DR SUPFAM; SSF46894; SSF46894; 1.
DR PROSITE; PS50043; HTH_LUXR_2; 1.
PE 2: Evidence at transcript level;
KW Activator; DNA-binding; Reference proteome; Transcription;
KW Transcription regulation.
FT CHAIN 1..281
FT /note="HTH-type transcriptional activator RamA"
FT /id="PRO_0000433052"
FT DOMAIN 213..278
FT /note="HTH luxR-type"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00411"
SQ SEQUENCE 281 AA; 30840 MW; 91284738E261F4C0 CRC64;
MDTQRIKDDE DAIRSALTSL KTATGIPVTM FATVLQDNRL QITQWVGLRT PALQNLVIEP
GVGVGGRVVA TRRPVGVSDY TRANVISHEK DSAIQDEGLH SIVAVPVIVH REIRGVLYVG
VHSAVRLGDT VIEEVTMTAR TLEQNLAINS ALRRNGVPDG RGSLKANRVM NGAEWEQVRS
THSKLRMLAN RVTDEDLRRD LEELCDQMVT PVRIKQTTKL SARELDVLAC VALGHTNVEA
AEEMGIGAET VKSYLRSVMR KLGAHTRYEA VNAARRIGAL P
