ID   RAN1_LITCT              Reviewed;          25 AA.
AC   P82741;
DT   16-JAN-2004, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2001, sequence version 1.
DT   03-AUG-2022, entry version 45.
DE   RecName: Full=Ranatuerin-1 {ECO:0000303|PubMed:9784389};
OS   Lithobates catesbeianus (American bullfrog) (Rana catesbeiana).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Amphibia;
OC   Batrachia; Anura; Neobatrachia; Ranoidea; Ranidae; Lithobates.
OX   NCBI_TaxID=8400;
RN   [1]
RP   PROTEIN SEQUENCE, FUNCTION, AND SUBCELLULAR LOCATION.
RC   TISSUE=Skin secretion;
RX   PubMed=9784389; DOI=10.1006/bbrc.1998.9362;
RA   Goraya J., Knoop F.C., Conlon J.M.;
RT   "Ranatuerins: antimicrobial peptides isolated from the skin of the American
RT   bullfrog, Rana catesbeiana.";
RL   Biochem. Biophys. Res. Commun. 250:589-592(1998).
CC   -!- FUNCTION: Antibacterial activity against Gram-positive bacterium
CC       S.aureus (MIC=50 uM) and Gram-negative bacterium E.coli (MIC=2 uM). Has
CC       activity against C.albicans (MIC=70 uM). Shows no detectable hemolytic
CC       activity towards human erythrocytes. {ECO:0000269|PubMed:9784389}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:9784389}.
CC   -!- TISSUE SPECIFICITY: Expressed by the skin glands.
CC       {ECO:0000305|PubMed:9784389}.
CC   -!- SIMILARITY: Belongs to the frog skin active peptide (FSAP) family.
CC       Brevinin subfamily. {ECO:0000305}.
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DR   AlphaFoldDB; P82741; -.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0050832; P:defense response to fungus; IEA:UniProtKB-KW.
DR   GO; GO:0050829; P:defense response to Gram-negative bacterium; IEA:UniProt.
DR   GO; GO:0031640; P:killing of cells of another organism; IEA:UniProtKB-KW.
DR   InterPro; IPR012521; Antimicrobial_frog_2.
DR   Pfam; PF08023; Antimicrobial_2; 1.
PE   1: Evidence at protein level;
KW   Amphibian defense peptide; Antibiotic; Antimicrobial;
KW   Direct protein sequencing; Disulfide bond; Fungicide; Secreted.
FT   PEPTIDE         1..25
FT                   /note="Ranatuerin-1"
FT                   /evidence="ECO:0000269|PubMed:9784389"
FT                   /id="PRO_0000043560"
FT   DISULFID        19..25
FT                   /evidence="ECO:0000305|PubMed:9784389"
SQ   SEQUENCE   25 AA;  2651 MW;  A490DF447E13B1C9 CRC64;
     SMLSVLKNLG KVGLGFVACK INKQC