RAN3_ARATH
ID RAN3_ARATH Reviewed; 221 AA.
AC Q8H156; O04148; O04664; O22495;
DT 27-SEP-2005, integrated into UniProtKB/Swiss-Prot.
DT 27-SEP-2005, sequence version 2.
DT 03-AUG-2022, entry version 142.
DE RecName: Full=GTP-binding nuclear protein Ran-3;
DE AltName: Full=Ras-related nuclear protein 3;
GN Name=RAN3; OrderedLocusNames=At5g55190; ORFNames=MCO15.14;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], AND INTERACTION WITH RANBP1A AND RANBP1B.
RX PubMed=9025305; DOI=10.1046/j.1365-313x.1997.11010093.x;
RA Haizel T., Merkle T., Pay A., Fejes E., Nagy F.;
RT "Characterization of proteins that interact with the GTP-bound form of the
RT regulatory GTPase Ran in Arabidopsis.";
RL Plant J. 11:93-103(1997).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=cv. Columbia;
RA Bischoff F., Palme K.;
RT "A new member of the Ran-GTPases in Arabidopsis thaliana.";
RL Submitted (OCT-1996) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=cv. Columbia;
RA Pih K.T., Park J.M., Jang H.J., Kang S.G., Piao H.L., Hwang I.H.;
RT "Isolation of salt stress inducible Ran1 isoform.";
RL Submitted (SEP-1998) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=9628582; DOI=10.1093/dnares/5.1.41;
RA Sato S., Kaneko T., Kotani H., Nakamura Y., Asamizu E., Miyajima N.,
RA Tabata S.;
RT "Structural analysis of Arabidopsis thaliana chromosome 5. IV. Sequence
RT features of the regions of 1,456,315 bp covered by nineteen physically
RT assigned P1 and TAC clones.";
RL DNA Res. 5:41-54(1998).
RN [5]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [6]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [7]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=12644670; DOI=10.1104/pp.013052;
RA Vernoud V., Horton A.C., Yang Z., Nielsen E.;
RT "Analysis of the small GTPase gene superfamily of Arabidopsis.";
RL Plant Physiol. 131:1191-1208(2003).
RN [8]
RP INDUCTION.
RX PubMed=18481083; DOI=10.1007/s00425-008-0745-x;
RA Lee Y., Kim M.-H., Kim S.-K., Kim S.-H.;
RT "Phytochrome-mediated differential gene expression of plant Ran/TC4 small
RT G-proteins.";
RL Planta 228:215-224(2008).
RN [9]
RP INTERACTION WITH KPNB1.
RX PubMed=23582042; DOI=10.1111/tpj.12207;
RA Luo Y., Wang Z., Ji H., Fang H., Wang S., Tian L., Li X.;
RT "An Arabidopsis homolog of importin beta1 is required for ABA response and
RT drought tolerance.";
RL Plant J. 75:377-389(2013).
CC -!- FUNCTION: GTP-binding protein involved in nucleocytoplasmic transport.
CC Required for the import of protein into the nucleus and also for RNA
CC export. Involved in chromatin condensation and control of cell cycle
CC (By similarity). {ECO:0000250}.
CC -!- SUBUNIT: Found in a nuclear export complex with RanGTP, exportin and
CC pre-miRNA (By similarity). Interacts with RanBP1a and RanBP1b
CC (PubMed:9025305). Interacts with KPNB1 (PubMed:23582042).
CC {ECO:0000250|UniProtKB:P62825, ECO:0000269|PubMed:23582042,
CC ECO:0000269|PubMed:9025305}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250}.
CC -!- INDUCTION: Induced by salt treatment. Regulated by light.
CC {ECO:0000269|PubMed:18481083}.
CC -!- SIMILARITY: Belongs to the small GTPase superfamily. Ran family.
CC {ECO:0000305}.
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DR EMBL; X97381; CAA66049.1; -; mRNA.
DR EMBL; U73810; AAB58478.1; -; mRNA.
DR EMBL; U75601; AAC34900.1; -; mRNA.
DR EMBL; AF017991; AAB97312.1; -; mRNA.
DR EMBL; AB010071; BAB08588.1; -; Genomic_DNA.
DR EMBL; CP002688; AED96598.1; -; Genomic_DNA.
DR EMBL; AY042796; AAK68736.1; -; mRNA.
DR EMBL; AY050317; AAK91334.1; -; mRNA.
DR EMBL; AY116939; AAM51573.1; -; mRNA.
DR EMBL; BT000723; AAN31865.1; -; mRNA.
DR RefSeq; NP_200330.1; NM_124901.5.
DR AlphaFoldDB; Q8H156; -.
DR SMR; Q8H156; -.
DR BioGRID; 20856; 8.
DR IntAct; Q8H156; 3.
DR STRING; 3702.AT5G55190.1; -.
DR PaxDb; Q8H156; -.
DR PRIDE; Q8H156; -.
DR EnsemblPlants; AT5G55190.1; AT5G55190.1; AT5G55190.
DR GeneID; 835612; -.
DR Gramene; AT5G55190.1; AT5G55190.1; AT5G55190.
DR KEGG; ath:AT5G55190; -.
DR Araport; AT5G55190; -.
DR TAIR; locus:2161600; AT5G55190.
DR eggNOG; KOG0096; Eukaryota.
DR HOGENOM; CLU_041217_13_0_1; -.
DR InParanoid; Q8H156; -.
DR OMA; IRFNIWD; -.
DR OrthoDB; 1083175at2759; -.
DR PhylomeDB; Q8H156; -.
DR PRO; PR:Q8H156; -.
DR Proteomes; UP000006548; Chromosome 5.
DR ExpressionAtlas; Q8H156; baseline and differential.
DR Genevisible; Q8H156; AT.
DR GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR GO; GO:0005794; C:Golgi apparatus; HDA:TAIR.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0009506; C:plasmodesma; HDA:TAIR.
DR GO; GO:0009536; C:plastid; HDA:TAIR.
DR GO; GO:0005525; F:GTP binding; ISS:TAIR.
DR GO; GO:0003924; F:GTPase activity; ISS:TAIR.
DR GO; GO:0003729; F:mRNA binding; IDA:TAIR.
DR GO; GO:0006606; P:protein import into nucleus; IBA:GO_Central.
DR GO; GO:0000054; P:ribosomal subunit export from nucleus; IBA:GO_Central.
DR Gene3D; 3.40.50.300; -; 1.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR002041; Ran_GTPase.
DR InterPro; IPR005225; Small_GTP-bd_dom.
DR InterPro; IPR001806; Small_GTPase.
DR PANTHER; PTHR24071; PTHR24071; 1.
DR Pfam; PF00071; Ras; 1.
DR PRINTS; PR00627; GTPRANTC4.
DR SMART; SM00174; RHO; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
DR TIGRFAMs; TIGR00231; small_GTP; 1.
DR PROSITE; PS51418; RAN; 1.
PE 1: Evidence at protein level;
KW GTP-binding; Nucleotide-binding; Nucleus; Protein transport;
KW Reference proteome; Transport.
FT CHAIN 1..221
FT /note="GTP-binding nuclear protein Ran-3"
FT /id="PRO_0000208719"
FT REGION 201..221
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT BINDING 21..28
FT /ligand="GTP"
FT /ligand_id="ChEBI:CHEBI:37565"
FT /evidence="ECO:0000250|UniProtKB:P62825"
FT BINDING 71
FT /ligand="GTP"
FT /ligand_id="ChEBI:CHEBI:37565"
FT /evidence="ECO:0000250|UniProtKB:P62825"
FT BINDING 125..128
FT /ligand="GTP"
FT /ligand_id="ChEBI:CHEBI:37565"
FT /evidence="ECO:0000250|UniProtKB:P62825"
FT BINDING 153..155
FT /ligand="GTP"
FT /ligand_id="ChEBI:CHEBI:37565"
FT /evidence="ECO:0000250|UniProtKB:P62825"
FT CONFLICT 38
FT /note="F -> P (in Ref. 1; CAA66049)"
FT /evidence="ECO:0000305"
FT CONFLICT 53
FT /note="L -> P (in Ref. 3; AAC34900/AAB97312)"
FT /evidence="ECO:0000305"
FT CONFLICT 80
FT /note="D -> E (in Ref. 6; AAN31865)"
FT /evidence="ECO:0000305"
FT CONFLICT 102
FT /note="K -> R (in Ref. 3; AAC34900/AAB97312)"
FT /evidence="ECO:0000305"
FT CONFLICT 145..146
FT /note="KN -> EE (in Ref. 3; AAC34900/AAB97312)"
FT /evidence="ECO:0000305"
FT CONFLICT 199
FT /note="Q -> R (in Ref. 1; CAA66049)"
FT /evidence="ECO:0000305"
FT CONFLICT 203..204
FT /note="EL -> DV (in Ref. 1; CAA66049)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 221 AA; 25080 MW; 96648D02606EDC0B CRC64;
MALPNQQTVD YPSFKLVIVG DGGTGKTTFV KRHLTGEFEK KYEPTIGVEV HPLDFFTNCG
KIRFYCWDTA GQEKFGGLRD GYYIHGQCAI IMFDVTARLT YKNVPTWHRD LCRVCENIPI
VLCGNKVDVK NRQVKAKQVT FHRKKNLQYY EISAKSNYNF EKPFLYLARK LAGDANLHFV
ESPALAPPEV QIDLAAQQQH EAELAAAASQ PLPDDDDDTF E
