ID   RAN4_LITCT              Reviewed;          24 AA.
AC   P82819;
DT   16-JAN-2004, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2001, sequence version 1.
DT   25-MAY-2022, entry version 43.
DE   RecName: Full=Ranatuerin-4 {ECO:0000303|PubMed:9784389};
OS   Lithobates catesbeianus (American bullfrog) (Rana catesbeiana).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Amphibia;
OC   Batrachia; Anura; Neobatrachia; Ranoidea; Ranidae; Lithobates.
OX   NCBI_TaxID=8400;
RN   [1]
RP   PROTEIN SEQUENCE, FUNCTION, AND SUBCELLULAR LOCATION.
RC   TISSUE=Skin secretion;
RX   PubMed=9784389; DOI=10.1006/bbrc.1998.9362;
RA   Goraya J., Knoop F.C., Conlon J.M.;
RT   "Ranatuerins: antimicrobial peptides isolated from the skin of the American
RT   bullfrog, Rana catesbeiana.";
RL   Biochem. Biophys. Res. Commun. 250:589-592(1998).
CC   -!- FUNCTION: Antibacterial activity against Gram-positive bacterium
CC       S.aureus (MIC=55 uM). Shows no detectable hemolytic activity towards
CC       human erythrocytes. {ECO:0000269|PubMed:9784389}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:9784389}.
CC   -!- TISSUE SPECIFICITY: Expressed by the skin glands.
CC       {ECO:0000305|PubMed:9784389}.
CC   -!- SIMILARITY: Belongs to the frog skin active peptide (FSAP) family.
CC       Brevinin subfamily. {ECO:0000305}.
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DR   AlphaFoldDB; P82819; -.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0042742; P:defense response to bacterium; IEA:UniProtKB-KW.
DR   InterPro; IPR012520; Antimicrobial_frog_1.
DR   Pfam; PF08018; Antimicrobial_1; 1.
PE   1: Evidence at protein level;
KW   Amphibian defense peptide; Antibiotic; Antimicrobial;
KW   Direct protein sequencing; Disulfide bond; Secreted.
FT   PEPTIDE         1..24
FT                   /note="Ranatuerin-4"
FT                   /evidence="ECO:0000269|PubMed:9784389"
FT                   /id="PRO_0000043561"
FT   DISULFID        18..24
FT                   /evidence="ECO:0000305|PubMed:9784389"
SQ   SEQUENCE   24 AA;  2653 MW;  098C43F9F09F7DFD CRC64;
     FLPFIARLAA KVFPSIICSV TKKC