ID   RANA_LITPI              Reviewed;          82 AA.
AC   P08950;
DT   01-NOV-1988, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1988, sequence version 1.
DT   25-MAY-2022, entry version 65.
DE   RecName: Full=Ranatensin;
DE   Flags: Precursor;
OS   Lithobates pipiens (Northern leopard frog) (Rana pipiens).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Amphibia;
OC   Batrachia; Anura; Neobatrachia; Ranoidea; Ranidae; Lithobates.
OX   NCBI_TaxID=8404;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   TISSUE=Skin;
RX   PubMed=2458345; DOI=10.1016/s0021-9258(18)37707-x;
RA   Krane I.M., Naylor S.L., Helin-Davis D., Chin W.W., Spindel E.R.;
RT   "Molecular cloning of cDNAs encoding the human bombesin-like peptide
RT   neuromedin B. Chromosomal localization and comparison to cDNAs encoding its
RT   amphibian homolog ranatensin.";
RL   J. Biol. Chem. 263:13317-13323(1988).
RN   [2]
RP   PROTEIN SEQUENCE OF 48-58, AND AMIDATION AT MET-58.
RA   Nakajima T., Tanimura T., Pisano J.J.;
RT   "Isolation and structure of a new vasoactive polypeptide.";
RL   Fed. Proc. 29:282-282(1970).
CC   -!- SUBCELLULAR LOCATION: Secreted.
CC   -!- TISSUE SPECIFICITY: Expressed by the skin glands.
CC   -!- SIMILARITY: Belongs to the bombesin/neuromedin-B/ranatensin family.
CC       {ECO:0000305}.
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DR   EMBL; M21552; AAA49533.1; -; mRNA.
DR   PIR; B28945; B28945.
DR   AlphaFoldDB; P08950; -.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0006952; P:defense response; IEA:UniProtKB-KW.
DR   GO; GO:0007218; P:neuropeptide signaling pathway; IEA:InterPro.
DR   InterPro; IPR000874; Bombesin.
DR   Pfam; PF02044; Bombesin; 1.
DR   PROSITE; PS00257; BOMBESIN; 1.
PE   1: Evidence at protein level;
KW   Amidation; Amphibian defense peptide; Cleavage on pair of basic residues;
KW   Direct protein sequencing; Secreted; Signal.
FT   SIGNAL          1..27
FT                   /evidence="ECO:0000255"
FT   PROPEP          28..47
FT                   /evidence="ECO:0000269|Ref.2"
FT                   /id="PRO_0000003027"
FT   PEPTIDE         48..58
FT                   /note="Ranatensin"
FT                   /id="PRO_0000003028"
FT   PROPEP          62..82
FT                   /id="PRO_0000003029"
FT   MOD_RES         58
FT                   /note="Methionine amide"
FT                   /evidence="ECO:0000269|Ref.2"
SQ   SEQUENCE   82 AA;  9159 MW;  09194FFD0E7436DE CRC64;
     MTTIPAIGIL PIDFLTILLL FSFISHSVCV EFAEDAGELD KSNAFRRQVP QWAVGHFMGK
     RSLSDDTEQA TMYSSRFVES TS