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RAN_CHICK
ID   RAN_CHICK               Reviewed;         216 AA.
AC   P42558;
DT   01-NOV-1995, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1995, sequence version 1.
DT   03-AUG-2022, entry version 151.
DE   RecName: Full=GTP-binding nuclear protein Ran;
DE            EC=3.6.5.- {ECO:0000250|UniProtKB:P62826};
DE   AltName: Full=GTPase Ran;
DE   AltName: Full=Ras-like protein TC4;
DE   AltName: Full=Ras-related nuclear protein;
GN   Name=RAN;
OS   Gallus gallus (Chicken).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Archelosauria; Archosauria; Dinosauria; Saurischia; Theropoda;
OC   Coelurosauria; Aves; Neognathae; Galloanserae; Galliformes; Phasianidae;
OC   Phasianinae; Gallus.
OX   NCBI_TaxID=9031;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RX   PubMed=1633874; DOI=10.1016/0014-5793(92)80995-s;
RA   Trueb J., Trueb B.;
RT   "Molecular cloning of a novel ras-like protein from chicken.";
RL   FEBS Lett. 306:181-184(1992).
CC   -!- FUNCTION: GTPase involved in nucleocytoplasmic transport, participating
CC       both to the import and the export from the nucleus of proteins and
CC       RNAs. Switches between a cytoplasmic GDP- and a nuclear GTP-bound state
CC       by nucleotide exchange and GTP hydrolysis. Nuclear import receptors
CC       such as importin beta bind their substrates only in the absence of GTP-
CC       bound RAN and release them upon direct interaction with GTP-bound RAN,
CC       while export receptors behave in the opposite way. Thereby, RAN
CC       controls cargo loading and release by transport receptors in the proper
CC       compartment and ensures the directionality of the transport.
CC       Interaction with RANBP1 induces a conformation change in the complex
CC       formed by XPO1 and RAN that triggers the release of the nuclear export
CC       signal of cargo proteins. RAN (GTP-bound form) triggers microtubule
CC       assembly at mitotic chromosomes and is required for normal mitotic
CC       spindle assembly and chromosome segregation. Required for normal
CC       progress through mitosis. {ECO:0000250|UniProtKB:P62826}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=GTP + H2O = GDP + H(+) + phosphate; Xref=Rhea:RHEA:19669,
CC         ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:37565,
CC         ChEBI:CHEBI:43474, ChEBI:CHEBI:58189;
CC         Evidence={ECO:0000250|UniProtKB:P62826};
CC       PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:19670;
CC         Evidence={ECO:0000250|UniProtKB:P62826};
CC   -!- COFACTOR:
CC       Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC         Evidence={ECO:0000250|UniProtKB:P62826};
CC       Note=Mg(2+) interacts primarily with the phosphate groups of the bound
CC       guanine nucleotide. {ECO:0000250|UniProtKB:P62826};
CC   -!- SUBUNIT: Monomer. Interacts with RANGAP1, which promotes RAN-mediated
CC       GTP hydrolysis. Interacts with KPNB1. Interaction with KPNB1 inhibits
CC       RANGAP1-mediated stimulation of GTPase activity. Interacts with RCC1
CC       which promotes the exchange of RAN-bound GDP by GTP. Interaction with
CC       KPNB1 inhibits RCC1-mediated exchange of RAN-bound GDP by GTP.
CC       Interacts (GTP-bound form) with TNPO1; the interaction is direct.
CC       Interacts (GTP-bound form) with TNPO3; the interaction is direct.
CC       Interacts with KPNB1 and with TNPO1; both inhibit RAN GTPase activity.
CC       Interacts (via C-terminus) with RANBP1, which alleviates the inhibition
CC       of RAN GTPase activity. Interacts with RANGRF, which promotes the
CC       release of bound guanine nucleotide. RANGRF and RCC1 compete for an
CC       overlapping binding site on RAN. Identified in a complex with KPNA2 and
CC       CSE1L; interaction with RANBP1 mediates dissociation of RAN from this
CC       complex. Interaction with both RANBP1 and KPNA2 promotes dissociation
CC       of the complex between RAN and KPNB1. Identified in a complex composed
CC       of RAN, RANGAP1 and RANBP1. Identified in a complex that contains
CC       TNPO1, RAN and RANBP1. Identified in a nuclear export complex with
CC       XPO1. Interaction with RANBP1 or RANBP2 induces a conformation change
CC       in the complex formed by XPO1 and RAN that triggers the release of the
CC       nuclear export signal of cargo proteins. Component of a nuclear export
CC       receptor complex composed of KPNB1, RAN, SNUPN and XPO1.
CC       {ECO:0000250|UniProtKB:P62825, ECO:0000250|UniProtKB:P62826,
CC       ECO:0000250|UniProtKB:P62827}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250|UniProtKB:P62826}. Nucleus
CC       envelope {ECO:0000250|UniProtKB:P62826}. Cytoplasm, cytosol
CC       {ECO:0000250|UniProtKB:P62826}. Cytoplasm
CC       {ECO:0000250|UniProtKB:P62826}. Note=Predominantly nuclear during
CC       interphase. Becomes dispersed throughout the cytoplasm during mitosis
CC       (By similarity). {ECO:0000250|UniProtKB:P62826}.
CC   -!- SIMILARITY: Belongs to the small GTPase superfamily. Ran family.
CC       {ECO:0000305}.
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DR   EMBL; X66906; CAA47355.1; -; mRNA.
DR   PIR; S24031; S24031.
DR   RefSeq; NP_990589.1; NM_205258.2.
DR   AlphaFoldDB; P42558; -.
DR   BMRB; P42558; -.
DR   SMR; P42558; -.
DR   BioGRID; 676453; 2.
DR   STRING; 9031.ENSGALP00000004046; -.
DR   PaxDb; P42558; -.
DR   Ensembl; ENSGALT00000004055; ENSGALP00000004046; ENSGALG00000002569.
DR   Ensembl; ENSGALT00000055724; ENSGALP00000054241; ENSGALG00000002569.
DR   GeneID; 396193; -.
DR   KEGG; gga:396193; -.
DR   CTD; 5901; -.
DR   VEuPathDB; HostDB:geneid_396193; -.
DR   eggNOG; KOG0096; Eukaryota.
DR   GeneTree; ENSGT00940000153786; -.
DR   HOGENOM; CLU_041217_13_0_1; -.
DR   InParanoid; P42558; -.
DR   OMA; TIVFHRK; -.
DR   OrthoDB; 1033833at2759; -.
DR   PhylomeDB; P42558; -.
DR   Reactome; R-GGA-1655829; Regulation of cholesterol biosynthesis by SREBP (SREBF).
DR   Reactome; R-GGA-5578749; Transcriptional regulation by small RNAs.
DR   Reactome; R-GGA-9615933; Postmitotic nuclear pore complex (NPC) reformation.
DR   PRO; PR:P42558; -.
DR   Proteomes; UP000000539; Chromosome 15.
DR   Bgee; ENSGALG00000002569; Expressed in testis and 13 other tissues.
DR   ExpressionAtlas; P42558; baseline and differential.
DR   GO; GO:0005814; C:centriole; IEA:Ensembl.
DR   GO; GO:0000785; C:chromatin; IEA:Ensembl.
DR   GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR   GO; GO:0005829; C:cytosol; IEA:UniProtKB-SubCell.
DR   GO; GO:0030496; C:midbody; IEA:Ensembl.
DR   GO; GO:0005635; C:nuclear envelope; IEA:UniProtKB-SubCell.
DR   GO; GO:0005730; C:nucleolus; IEA:Ensembl.
DR   GO; GO:0005654; C:nucleoplasm; IEA:Ensembl.
DR   GO; GO:0005634; C:nucleus; ISS:UniProtKB.
DR   GO; GO:0055037; C:recycling endosome; IEA:Ensembl.
DR   GO; GO:0042565; C:RNA nuclear export complex; IEA:Ensembl.
DR   GO; GO:0019003; F:GDP binding; IEA:Ensembl.
DR   GO; GO:0005525; F:GTP binding; ISS:UniProtKB.
DR   GO; GO:0003924; F:GTPase activity; ISS:UniProtKB.
DR   GO; GO:0000287; F:magnesium ion binding; ISS:UniProtKB.
DR   GO; GO:0070883; F:pre-miRNA binding; IEA:Ensembl.
DR   GO; GO:0046982; F:protein heterodimerization activity; IEA:Ensembl.
DR   GO; GO:0046039; P:GTP metabolic process; ISS:UniProtKB.
DR   GO; GO:0000070; P:mitotic sister chromatid segregation; ISS:UniProtKB.
DR   GO; GO:0032092; P:positive regulation of protein binding; IEA:Ensembl.
DR   GO; GO:0042307; P:positive regulation of protein import into nucleus; IEA:Ensembl.
DR   GO; GO:0006611; P:protein export from nucleus; IEA:Ensembl.
DR   GO; GO:0006606; P:protein import into nucleus; ISS:UniProtKB.
DR   GO; GO:1902570; P:protein localization to nucleolus; IEA:Ensembl.
DR   GO; GO:0000055; P:ribosomal large subunit export from nucleus; IEA:Ensembl.
DR   GO; GO:0000056; P:ribosomal small subunit export from nucleus; IEA:Ensembl.
DR   GO; GO:0000054; P:ribosomal subunit export from nucleus; IBA:GO_Central.
DR   GO; GO:0061015; P:snRNA import into nucleus; ISS:UniProtKB.
DR   Gene3D; 3.40.50.300; -; 1.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   InterPro; IPR002041; Ran_GTPase.
DR   InterPro; IPR005225; Small_GTP-bd_dom.
DR   InterPro; IPR001806; Small_GTPase.
DR   PANTHER; PTHR24071; PTHR24071; 1.
DR   Pfam; PF00071; Ras; 1.
DR   PRINTS; PR00627; GTPRANTC4.
DR   SMART; SM00174; RHO; 1.
DR   SUPFAM; SSF52540; SSF52540; 1.
DR   TIGRFAMs; TIGR00231; small_GTP; 1.
DR   PROSITE; PS51418; RAN; 1.
PE   2: Evidence at transcript level;
KW   Cytoplasm; GTP-binding; Hydrolase; Magnesium; Metal-binding;
KW   Nucleotide-binding; Nucleus; Protein transport; Reference proteome;
KW   Transport.
FT   CHAIN           1..216
FT                   /note="GTP-binding nuclear protein Ran"
FT                   /id="PRO_0000208701"
FT   REGION          211..216
FT                   /note="Interaction with RANBP1"
FT                   /evidence="ECO:0000250|UniProtKB:P62826"
FT   BINDING         18..25
FT                   /ligand="GTP"
FT                   /ligand_id="ChEBI:CHEBI:37565"
FT                   /evidence="ECO:0000250|UniProtKB:P62825"
FT   BINDING         36..42
FT                   /ligand="GTP"
FT                   /ligand_id="ChEBI:CHEBI:37565"
FT                   /evidence="ECO:0000250|UniProtKB:P62825"
FT   BINDING         68
FT                   /ligand="GTP"
FT                   /ligand_id="ChEBI:CHEBI:37565"
FT                   /evidence="ECO:0000250|UniProtKB:P62825"
FT   BINDING         122..125
FT                   /ligand="GTP"
FT                   /ligand_id="ChEBI:CHEBI:37565"
FT                   /evidence="ECO:0000250|UniProtKB:P62825"
FT   BINDING         150..152
FT                   /ligand="GTP"
FT                   /ligand_id="ChEBI:CHEBI:37565"
FT                   /evidence="ECO:0000250|UniProtKB:P62825"
FT   SITE            69
FT                   /note="Essential for GTP hydrolysis"
FT                   /evidence="ECO:0000250|UniProtKB:P62826"
SQ   SEQUENCE   216 AA;  24427 MW;  D5DB00D75C2C0EA4 CRC64;
     MAAQGEPQVQ FKLVLVGDGG TGKTTFVKRH LTGEFEKKYV ATLGVEVHPL VFHTNRGPIK
     FNVWDTAGQE KFGGLRDGYY IQAQCAIIMF DVTSRVTYKN VPNWHRDLVR VCENIPIVLC
     GNKVDIKDRK VKAKSIVFHR KKNLQYYDIS AKSNYNFEKP FLWLARKLIG DPNLEFVAMP
     ALAPPEVVMD PALAAQYEQD LQIAQTTALP DEDDDL
 
 
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