RAP24_ARATH
ID RAP24_ARATH Reviewed; 334 AA.
AC Q8H1E4; O23106; Q94K95; Q9C9S5; Q9SGZ3;
DT 21-AUG-2007, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2003, sequence version 1.
DT 03-AUG-2022, entry version 130.
DE RecName: Full=Ethylene-responsive transcription factor RAP2-4;
DE AltName: Full=Ethylene-responsive transcription factor ERF059;
DE AltName: Full=Protein RELATED TO APETALA2 4;
GN Name=RAP2-4; Synonyms=ERF059; OrderedLocusNames=At1g78080;
GN ORFNames=F28K19.29, T11I11.1;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [4]
RP NUCLEOTIDE SEQUENCE [MRNA] OF 106-334, AND TISSUE SPECIFICITY.
RX PubMed=9192694; DOI=10.1073/pnas.94.13.7076;
RA Okamuro J.K., Caster B., Villarroel R., Van Montagu M., Jofuku K.D.;
RT "The AP2 domain of APETALA2 defines a large new family of DNA binding
RT proteins in Arabidopsis.";
RL Proc. Natl. Acad. Sci. U.S.A. 94:7076-7081(1997).
RN [5]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=16407444; DOI=10.1104/pp.105.073783;
RA Nakano T., Suzuki K., Fujimura T., Shinshi H.;
RT "Genome-wide analysis of the ERF gene family in Arabidopsis and rice.";
RL Plant Physiol. 140:411-432(2006).
RN [6]
RP INTERACTION WITH BPM PROTEINS.
RX PubMed=19843165; DOI=10.1111/j.1742-4658.2009.07373.x;
RA Weber H., Hellmann H.;
RT "Arabidopsis thaliana BTB/ POZ-MATH proteins interact with members of the
RT ERF/AP2 transcription factor family.";
RL FEBS J. 276:6624-6635(2009).
CC -!- FUNCTION: Probably acts as a transcriptional activator. Binds to the
CC GCC-box pathogenesis-related promoter element. May be involved in the
CC regulation of gene expression by stress factors and by components of
CC stress signal transduction pathways (By similarity). {ECO:0000250}.
CC -!- SUBUNIT: Interacts with BTB/POZ-MATH proteins BPM1, BPM2, BPM3, BPM4,
CC BPM5 and BPM6. {ECO:0000269|PubMed:19843165}.
CC -!- INTERACTION:
CC Q8H1E4; Q8L765: BPM1; NbExp=5; IntAct=EBI-7528315, EBI-540891;
CC Q8H1E4; O22286: BPM3; NbExp=2; IntAct=EBI-7528315, EBI-540923;
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}.
CC -!- TISSUE SPECIFICITY: Mostly expressed in roots and leaves. Also detected
CC in flowers and stems. {ECO:0000269|PubMed:9192694}.
CC -!- SIMILARITY: Belongs to the AP2/ERF transcription factor family. ERF
CC subfamily. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAF17691.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR EMBL; AC009243; AAF17691.1; ALT_INIT; Genomic_DNA.
DR EMBL; AC012680; AAG52091.1; -; Genomic_DNA.
DR EMBL; CP002684; AEE36065.1; -; Genomic_DNA.
DR EMBL; AF370152; AAK43967.1; -; mRNA.
DR EMBL; AY150468; AAN12993.1; -; mRNA.
DR EMBL; AF003097; AAC49770.1; -; mRNA.
DR RefSeq; NP_177931.1; NM_106457.3.
DR AlphaFoldDB; Q8H1E4; -.
DR SMR; Q8H1E4; -.
DR BioGRID; 29362; 15.
DR IntAct; Q8H1E4; 15.
DR MINT; Q8H1E4; -.
DR STRING; 3702.AT1G78080.1; -.
DR PaxDb; Q8H1E4; -.
DR PRIDE; Q8H1E4; -.
DR EnsemblPlants; AT1G78080.1; AT1G78080.1; AT1G78080.
DR GeneID; 844143; -.
DR Gramene; AT1G78080.1; AT1G78080.1; AT1G78080.
DR KEGG; ath:AT1G78080; -.
DR Araport; AT1G78080; -.
DR TAIR; locus:2029491; AT1G78080.
DR eggNOG; ENOG502QQEY; Eukaryota.
DR HOGENOM; CLU_057028_1_0_1; -.
DR InParanoid; Q8H1E4; -.
DR OMA; DMCSENY; -.
DR OrthoDB; 1050878at2759; -.
DR PhylomeDB; Q8H1E4; -.
DR PRO; PR:Q8H1E4; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; Q8H1E4; baseline and differential.
DR Genevisible; Q8H1E4; AT.
DR GO; GO:0005634; C:nucleus; IDA:TAIR.
DR GO; GO:0003677; F:DNA binding; IDA:TAIR.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; IDA:TAIR.
DR GO; GO:0043565; F:sequence-specific DNA binding; IDA:TAIR.
DR GO; GO:0000976; F:transcription cis-regulatory region binding; IPI:TAIR.
DR GO; GO:0071472; P:cellular response to salt stress; IEP:TAIR.
DR GO; GO:0009736; P:cytokinin-activated signaling pathway; IMP:TAIR.
DR GO; GO:0009873; P:ethylene-activated signaling pathway; IMP:TAIR.
DR GO; GO:0045595; P:regulation of cell differentiation; IMP:TAIR.
DR GO; GO:0009409; P:response to cold; IEP:TAIR.
DR GO; GO:0009408; P:response to heat; IEP:TAIR.
DR GO; GO:0009416; P:response to light stimulus; IEP:TAIR.
DR GO; GO:0006970; P:response to osmotic stress; IEP:TAIR.
DR GO; GO:0009651; P:response to salt stress; IEP:TAIR.
DR GO; GO:0009414; P:response to water deprivation; IMP:TAIR.
DR GO; GO:0009611; P:response to wounding; IEP:TAIR.
DR CDD; cd00018; AP2; 1.
DR Gene3D; 3.30.730.10; -; 1.
DR InterPro; IPR001471; AP2/ERF_dom.
DR InterPro; IPR036955; AP2/ERF_dom_sf.
DR InterPro; IPR016177; DNA-bd_dom_sf.
DR Pfam; PF00847; AP2; 1.
DR PRINTS; PR00367; ETHRSPELEMNT.
DR SMART; SM00380; AP2; 1.
DR SUPFAM; SSF54171; SSF54171; 1.
DR PROSITE; PS51032; AP2_ERF; 1.
PE 1: Evidence at protein level;
KW Activator; DNA-binding; Ethylene signaling pathway; Nucleus;
KW Reference proteome; Transcription; Transcription regulation.
FT CHAIN 1..334
FT /note="Ethylene-responsive transcription factor RAP2-4"
FT /id="PRO_0000297934"
FT DNA_BIND 151..208
FT /note="AP2/ERF"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00366"
FT REGION 243..317
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 246..273
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CONFLICT 122
FT /note="S -> R (in Ref. 3; AAK43967)"
FT /evidence="ECO:0000305"
FT CONFLICT 215
FT /note="S -> F (in Ref. 4; AAC49770)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 334 AA; 36610 MW; E0A19F1DB535C372 CRC64;
MAAAMNLYTC SRSFQDSGGE LMDALVPFIK SVSDSPSSSS AASASAFLHP SAFSLPPLPG
YYPDSTFLTQ PFSYGSDLQQ TGSLIGLNNL SSSQIHQIQS QIHHPLPPTH HNNNNSFSNL
LSPKPLLMKQ SGVAGSCFAY GSGVPSKPTK LYRGVRQRHW GKWVAEIRLP RNRTRLWLGT
FDTAEEAALA YDKAAYKLRG DFARLNFPNL RHNGSHIGGD FGEYKPLHSS VDAKLEAICK
SMAETQKQDK STKSSKKREK KVSSPDLSEK VKAEENSVSI GGSPPVTEFE ESTAGSSPLS
DLTFADPEEP PQWNETFSLE KYPSYEIDWD SILA
