RAP27_ARATH
ID RAP27_ARATH Reviewed; 449 AA.
AC Q9SK03; O23109; Q3E7W2; Q9C5R2;
DT 21-AUG-2007, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2002, sequence version 2.
DT 03-AUG-2022, entry version 133.
DE RecName: Full=Ethylene-responsive transcription factor RAP2-7;
DE AltName: Full=Protein RELATED TO APETALA2 7;
DE AltName: Full=Protein TARGET OF EAT 1;
GN Name=RAP2-7; Synonyms=TOE1; OrderedLocusNames=At2g28550;
GN ORFNames=T17D12.11;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617197; DOI=10.1038/45471;
RA Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D.,
RA Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V.,
RA Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S.,
RA Cronin L.A., Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J.,
RA Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M.,
RA Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O.,
RA Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.;
RT "Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana.";
RL Nature 402:761-768(1999).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC STRAIN=cv. Columbia;
RA Southwick A.M., Karlin-Neumann G., Nguyen M., Lam B., Miranda M.,
RA Palm C.J., Theologis A., Ecker J.R., Davis R.W.;
RL Submitted (NOV-2000) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP NUCLEOTIDE SEQUENCE [MRNA] OF 47-449 (ISOFORM 1).
RX PubMed=9192694; DOI=10.1073/pnas.94.13.7076;
RA Okamuro J.K., Caster B., Villarroel R., Van Montagu M., Jofuku K.D.;
RT "The AP2 domain of APETALA2 defines a large new family of DNA binding
RT proteins in Arabidopsis.";
RL Proc. Natl. Acad. Sci. U.S.A. 94:7076-7081(1997).
RN [6]
RP FUNCTION, AND INDUCTION.
RX PubMed=14555699; DOI=10.1105/tpc.016238;
RA Aukerman M.J., Sakai H.;
RT "Regulation of flowering time and floral organ identity by a microRNA and
RT its APETALA2-like target genes.";
RL Plant Cell 15:2730-2741(2003).
RN [7]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=16407444; DOI=10.1104/pp.105.073783;
RA Nakano T., Suzuki K., Fujimura T., Shinshi H.;
RT "Genome-wide analysis of the ERF gene family in Arabidopsis and rice.";
RL Plant Physiol. 140:411-432(2006).
CC -!- FUNCTION: Probably acts as a transcriptional activator. Binds to the
CC GCC-box pathogenesis-related promoter element. May be involved in the
CC regulation of gene expression by stress factors and by components of
CC stress signal transduction pathways (By similarity). Regulates
CC negatively the transition to flowering time and confers flowering time
CC delay. {ECO:0000250, ECO:0000269|PubMed:14555699}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q9SK03-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q9SK03-2; Sequence=VSP_027408, VSP_027409;
CC -!- INDUCTION: Repressed by miR172a-2/EAT. {ECO:0000269|PubMed:14555699}.
CC -!- SIMILARITY: Belongs to the AP2/ERF transcription factor family. AP2
CC subfamily. {ECO:0000305}.
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DR EMBL; AC006587; AAD21489.2; -; Genomic_DNA.
DR EMBL; CP002685; AEC08137.1; -; Genomic_DNA.
DR EMBL; AY050815; AAK92750.1; -; mRNA.
DR EMBL; AY091418; AAM14357.1; -; mRNA.
DR EMBL; AF325074; AAK17142.1; -; mRNA.
DR EMBL; AF003100; AAC49773.1; -; mRNA.
DR PIR; C84686; C84686.
DR RefSeq; NP_565674.1; NM_128415.5. [Q9SK03-1]
DR AlphaFoldDB; Q9SK03; -.
DR SMR; Q9SK03; -.
DR BioGRID; 2753; 33.
DR IntAct; Q9SK03; 14.
DR STRING; 3702.AT2G28550.3; -.
DR iPTMnet; Q9SK03; -.
DR PaxDb; Q9SK03; -.
DR PRIDE; Q9SK03; -.
DR EnsemblPlants; AT2G28550.1; AT2G28550.1; AT2G28550. [Q9SK03-1]
DR GeneID; 817403; -.
DR Gramene; AT2G28550.1; AT2G28550.1; AT2G28550. [Q9SK03-1]
DR KEGG; ath:AT2G28550; -.
DR Araport; AT2G28550; -.
DR eggNOG; ENOG502QSBE; Eukaryota.
DR InParanoid; Q9SK03; -.
DR PhylomeDB; Q9SK03; -.
DR PRO; PR:Q9SK03; -.
DR Proteomes; UP000006548; Chromosome 2.
DR ExpressionAtlas; Q9SK03; baseline and differential.
DR Genevisible; Q9SK03; AT.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; IEA:InterPro.
DR GO; GO:0009873; P:ethylene-activated signaling pathway; IEA:UniProtKB-KW.
DR CDD; cd00018; AP2; 1.
DR Gene3D; 3.30.730.10; -; 1.
DR InterPro; IPR001471; AP2/ERF_dom.
DR InterPro; IPR036955; AP2/ERF_dom_sf.
DR InterPro; IPR016177; DNA-bd_dom_sf.
DR Pfam; PF00847; AP2; 1.
DR SMART; SM00380; AP2; 2.
DR SUPFAM; SSF54171; SSF54171; 2.
DR PROSITE; PS51032; AP2_ERF; 1.
PE 2: Evidence at transcript level;
KW Activator; Alternative splicing; DNA-binding; Ethylene signaling pathway;
KW Nucleus; Reference proteome; Repeat; Transcription;
KW Transcription regulation.
FT CHAIN 1..449
FT /note="Ethylene-responsive transcription factor RAP2-7"
FT /id="PRO_0000297936"
FT DNA_BIND 153..209
FT /note="AP2/ERF 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00366"
FT DNA_BIND 243..292
FT /note="AP2/ERF 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00366"
FT REGION 1..52
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 124..153
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 393..449
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 7..52
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VAR_SEQ 379..381
FT /note="GRA -> VKT (in isoform 2)"
FT /evidence="ECO:0000305"
FT /id="VSP_027408"
FT VAR_SEQ 382..449
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000305"
FT /id="VSP_027409"
SQ SEQUENCE 449 AA; 49429 MW; B6F8B3CAA052CBD2 CRC64;
MLDLNLNADS PESTQYGGDS YLDRQTSDNS AGNRVEESGT STSSVINADG DEDSCSTRAF
TLSFDILKVG SSSGGDESPA ASASVTKEFF PVSGDCGHLR DVEGSSSSRN WIDLSFDRIG
DGETKLVTPV PTPAPVPAQV KKSRRGPRSR SSQYRGVTFY RRTGRWESHI WDCGKQVYLG
GFDTAHAAAR AYDRAAIKFR GVDADINFTL GDYEEDMKQV QNLSKEEFVH ILRRQSTGFS
RGSSKYRGVT LHKCGRWEAR MGQFLGKKAY DKAAINTNGR EAVTNFEMSS YQNEINSESN
NSEIDLNLGI SLSTGNAPKQ NGRLFHFPSN TYETQRGVSL RIDNEYMGKP VNTPLPYGSS
DHRLYWNGAC PSYNNPAEGR ATEKRSEAEG MMSNWGWQRP GQTSAVRPQP PGPQPPPLFS
VAAASSGFSH FRPQPPNDNA TRGYFYPHP
