RAP94_VAR67
ID RAP94_VAR67 Reviewed; 795 AA.
AC P0DSR3; P33067;
DT 16-OCT-2019, integrated into UniProtKB/Swiss-Prot.
DT 16-OCT-2019, sequence version 1.
DT 29-SEP-2021, entry version 8.
DE RecName: Full=RNA polymerase-associated transcription-specificity factor RAP94;
DE AltName: Full=Protein H4;
DE AltName: Full=RPO-associated protein of 94 kDa;
GN Name=RAP94; ORFNames=H4L;
OS Variola virus (isolate Human/India/Ind3/1967) (VARV) (Smallpox virus).
OC Viruses; Varidnaviria; Bamfordvirae; Nucleocytoviricota; Pokkesviricetes;
OC Chitovirales; Poxviridae; Chordopoxvirinae; Orthopoxvirus.
OX NCBI_TaxID=587200;
OH NCBI_TaxID=9606; Homo sapiens (Human).
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=8383392; DOI=10.1016/0168-1702(93)90110-9;
RA Shchelkunov S.N., Blinov V.M., Totmenin A.V., Marennikova S.S.,
RA Kolykhalov A.A., Frolov I.V., Chizhikov V.E., Gytorov V.V., Gashikov P.V.,
RA Belanov E.F., Belavin P.A., Resenchuk S.M., Andzhaparidze O.G.,
RA Sandakhchiev L.S.;
RT "Nucleotide sequence analysis of variola virus HindIII M, L, I genome
RT fragments.";
RL Virus Res. 27:25-35(1993).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=8384129; DOI=10.1016/0014-5793(93)80041-r;
RA Shchelkunov S.N., Blinov V.M., Sandakhchiev L.S.;
RT "Genes of variola and vaccinia viruses necessary to overcome the host
RT protective mechanisms.";
RL FEBS Lett. 319:80-83(1993).
CC -!- FUNCTION: DNA-directed RNA polymerase-associated factor required for
CC the transcription of viral early genes as well as for transcription
CC termination. Within minutes after virus entry, recruits the core RNA
CC polymerase, the early transcription factor (ETF) and other enzymes
CC needed for transcription initiation, elongation, and termination
CC thereby allowing synthesis of early mRNAs which are extruded through
CC pores in the core particle. Recruits the multifunctional J3 protein,
CC with poly(A) polymerase-stimulatory, cap nucleoside-2'-O-
CC methyltransferase, and transcription elongation activities. Interacts
CC with NPH-I, a DNA-dependent ATPase required for the termination of
CC early transcripts. Acts as a transcription termination factor by
CC binding, together with the capping enzyme/VTF, to the termination motif
CC 5'-UUUUUNU-3' in the nascent mRNA. Involved as well in the packaging of
CC RNA polymerase and other components needed for early transcription in
CC assembling virus particles (By similarity). {ECO:0000250}.
CC -!- SUBUNIT: Part of the early transcription complex composed of ETF,
CC RAP94, and the DNA-directed RNA polymerase. Interacts (via N-terminus)
CC with NPH-I. Interacts with J3. Interacts with ETF heterodimer (By
CC similarity). {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Virion {ECO:0000305}. Note=All the enzymes and
CC other proteins required to synthesize early mRNAs are packaged within
CC the virion core along with the DNA genome. {ECO:0000250}.
CC -!- INDUCTION: Expressed in the late phase of the viral replicative cycle.
CC -!- DOMAIN: Interacts with ETF via its N-terminus and with DNA-directed RNA
CC polymerase via its C-terminus. {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the poxviridae protein RAP94 family.
CC {ECO:0000305}.
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DR EMBL; X67119; CAA47586.1; -; Genomic_DNA.
DR EMBL; S55844; AAB24683.1; -; Genomic_DNA.
DR EMBL; X69198; CAA49028.1; -; Genomic_DNA.
DR PIR; S33101; S33101.
DR RefSeq; NP_042131.1; NC_001611.1.
DR SMR; P0DSR3; -.
DR GeneID; 1486442; -.
DR KEGG; vg:1486442; -.
DR Proteomes; UP000002060; Genome.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; IEA:InterPro.
DR GO; GO:0006353; P:DNA-templated transcription, termination; IEA:UniProtKB-KW.
DR InterPro; IPR004974; Pox_Rap94.
DR Pfam; PF03294; Pox_Rap94; 1.
PE 2: Evidence at transcript level;
KW Late protein; Reference proteome; Transcription; Transcription regulation;
KW Transcription termination; Virion.
FT CHAIN 1..795
FT /note="RNA polymerase-associated transcription-specificity
FT factor RAP94"
FT /id="PRO_0000099122"
FT REGION 1..196
FT /note="Interaction with NPH-I; required for transcription
FT termination"
FT /evidence="ECO:0000250"
FT REGION 235..256
FT /note="Interaction with J3"
FT /evidence="ECO:0000250"
SQ SEQUENCE 795 AA; 93661 MW; 9E50C3490619D55A CRC64;
MDSKETILIE IIPKIKAYLL DANISPKSYD DFISRNKNIF VINLYNVSAI TEEDIRLLYT
TIEQNIDAND QTLVAIFSYI GYKFEQTVKE EISTSLSLND KNTTDEMTYN LYDLFFNTLD
MYLRQKKISI LVNDDVRGDV IVSYKNSDLV SSFNAELEPE IKKIPFNMKN LLPYLEKNLD
QLRFSKKYLD FAYLCRHIGI PISKKKYNVR YVFLYKIDGL SIPIIIKDFL DVKYVYLENT
GKIYKNSFSE DHNNSLSDWG KVIIPLLKDR HLYSYIFLSS YHLHSYYTDL IAKDEPVFIK
RKKLDIIEID EPEAWKRDVK VEFAPCEHQI RLKEAMKVDA NYFTKINNFA NEFIYYEDGV
AYCRVCGINI PIFNLDAADV IKNTVIVSTF NKTIFLSEPY SYFVHSQRFI FNIIMSFDNI
MKSQTWVMKY NINRLILNFF IDINSRRQEY EKKFSSEIKR GLFFLRLSAN LFESQVSSTE
LFYVSKMLNL NYIVALVIIL NSSADFIVSY MKSKNKTVEE STLKYAISVV IYDFLVKTRI
CEKGSLDTIV LFTDVYTSIM PEELDLHFQR ITLELRKLVS IQRSALEPNY DVESRGEELP
LSTLKFFDTS TIIVKTMAPV HTYVEQKIVA PTPSVEPTDA SLKQFKELTC DEDIKILIRV
HDTNATKLVI FPSHLKIEIE RKKLIIPLKS LYITNTLKYY YSNSYLYIFR FGDPMPFEEE
LIDHEHAQYK INCYNILRYH LLPDSDVFVY FSNSLNREAL EYAFYIFLSK YVNVKQWIDE
NITRIRELYM INFNN