ID   RAP94_VARV              Reviewed;         795 AA.
AC   P0DSR4; P33067;
DT   16-OCT-2019, integrated into UniProtKB/Swiss-Prot.
DT   16-OCT-2019, sequence version 1.
DT   29-SEP-2021, entry version 11.
DE   RecName: Full=RNA polymerase-associated transcription-specificity factor RAP94;
DE   AltName: Full=Protein H4;
DE   AltName: Full=RPO-associated protein of 94 kDa;
GN   Name=RAP94; ORFNames=H4L;
OS   Variola virus.
OC   Viruses; Varidnaviria; Bamfordvirae; Nucleocytoviricota; Pokkesviricetes;
OC   Chitovirales; Poxviridae; Chordopoxvirinae; Orthopoxvirus.
OX   NCBI_TaxID=10255;
OH   NCBI_TaxID=9606; Homo sapiens (Human).
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=Bangladesh-1975;
RX   PubMed=8264798; DOI=10.1038/366748a0;
RA   Massung R.F., Esposito J.J., Liu L.I., Qi J., Utterback T.R., Knight J.C.,
RA   Aubin L., Yuran T.E., Parsons J.M., Loparev V.N., Selivanov N.A.,
RA   Cavallaro K.F., Kerlavage A.R., Mahy B.W.J., Venter J.C.;
RT   "Potential virulence determinants in terminal regions of variola smallpox
RT   virus genome.";
RL   Nature 366:748-751(1993).
CC   -!- FUNCTION: DNA-directed RNA polymerase-associated factor required for
CC       the transcription of viral early genes as well as for transcription
CC       termination. Within minutes after virus entry, recruits the core RNA
CC       polymerase, the early transcription factor (ETF) and other enzymes
CC       needed for transcription initiation, elongation, and termination
CC       thereby allowing synthesis of early mRNAs which are extruded through
CC       pores in the core particle. Recruits the multifunctional J3 protein,
CC       with poly(A) polymerase-stimulatory, cap nucleoside-2'-O-
CC       methyltransferase, and transcription elongation activities. Interacts
CC       with NPH-I, a DNA-dependent ATPase required for the termination of
CC       early transcripts. Acts as a transcription termination factor by
CC       binding, together with the capping enzyme/VTF, to the termination motif
CC       5'-UUUUUNU-3' in the nascent mRNA. Involved as well in the packaging of
CC       RNA polymerase and other components needed for early transcription in
CC       assembling virus particles (By similarity). {ECO:0000250}.
CC   -!- SUBUNIT: Part of the early transcription complex composed of ETF,
CC       RAP94, and the DNA-directed RNA polymerase. Interacts (via N-terminus)
CC       with NPH-I. Interacts with J3. Interacts with ETF heterodimer (By
CC       similarity). {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Virion {ECO:0000305}. Note=All the enzymes and
CC       other proteins required to synthesize early mRNAs are packaged within
CC       the virion core along with the DNA genome. {ECO:0000250}.
CC   -!- INDUCTION: Expressed in the late phase of the viral replicative cycle.
CC   -!- DOMAIN: Interacts with ETF via its N-terminus and with DNA-directed RNA
CC       polymerase via its C-terminus. {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the poxviridae protein RAP94 family.
CC       {ECO:0000305}.
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DR   EMBL; L22579; AAA60835.1; -; Genomic_DNA.
DR   PIR; T28525; T28525.
DR   RefSeq; NP_042131.1; NC_001611.1.
DR   SMR; P0DSR4; -.
DR   GeneID; 1486442; -.
DR   KEGG; vg:1486442; -.
DR   Proteomes; UP000119805; Genome.
DR   GO; GO:0003700; F:DNA-binding transcription factor activity; IEA:InterPro.
DR   GO; GO:0006353; P:DNA-templated transcription, termination; IEA:UniProtKB-KW.
DR   InterPro; IPR004974; Pox_Rap94.
DR   Pfam; PF03294; Pox_Rap94; 1.
PE   2: Evidence at transcript level;
KW   Late protein; Transcription; Transcription regulation;
KW   Transcription termination; Virion.
FT   CHAIN           1..795
FT                   /note="RNA polymerase-associated transcription-specificity
FT                   factor RAP94"
FT                   /id="PRO_0000448134"
FT   REGION          1..196
FT                   /note="Interaction with NPH-I; required for transcription
FT                   termination"
FT                   /evidence="ECO:0000250"
FT   REGION          235..256
FT                   /note="Interaction with J3"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   795 AA;  93661 MW;  9E50C3490619D55A CRC64;
     MDSKETILIE IIPKIKAYLL DANISPKSYD DFISRNKNIF VINLYNVSAI TEEDIRLLYT
     TIEQNIDAND QTLVAIFSYI GYKFEQTVKE EISTSLSLND KNTTDEMTYN LYDLFFNTLD
     MYLRQKKISI LVNDDVRGDV IVSYKNSDLV SSFNAELEPE IKKIPFNMKN LLPYLEKNLD
     QLRFSKKYLD FAYLCRHIGI PISKKKYNVR YVFLYKIDGL SIPIIIKDFL DVKYVYLENT
     GKIYKNSFSE DHNNSLSDWG KVIIPLLKDR HLYSYIFLSS YHLHSYYTDL IAKDEPVFIK
     RKKLDIIEID EPEAWKRDVK VEFAPCEHQI RLKEAMKVDA NYFTKINNFA NEFIYYEDGV
     AYCRVCGINI PIFNLDAADV IKNTVIVSTF NKTIFLSEPY SYFVHSQRFI FNIIMSFDNI
     MKSQTWVMKY NINRLILNFF IDINSRRQEY EKKFSSEIKR GLFFLRLSAN LFESQVSSTE
     LFYVSKMLNL NYIVALVIIL NSSADFIVSY MKSKNKTVEE STLKYAISVV IYDFLVKTRI
     CEKGSLDTIV LFTDVYTSIM PEELDLHFQR ITLELRKLVS IQRSALEPNY DVESRGEELP
     LSTLKFFDTS TIIVKTMAPV HTYVEQKIVA PTPSVEPTDA SLKQFKELTC DEDIKILIRV
     HDTNATKLVI FPSHLKIEIE RKKLIIPLKS LYITNTLKYY YSNSYLYIFR FGDPMPFEEE
     LIDHEHAQYK INCYNILRYH LLPDSDVFVY FSNSLNREAL EYAFYIFLSK YVNVKQWIDE
     NITRIRELYM INFNN