ID   RAPA2_RHIL3             Reviewed;         236 AA.
AC   Q1M755;
DT   17-FEB-2016, integrated into UniProtKB/Swiss-Prot.
DT   30-MAY-2006, sequence version 1.
DT   25-MAY-2022, entry version 63.
DE   RecName: Full=Calcium-binding lectin RapA2 {ECO:0000303|PubMed:23235153};
GN   Name=rapA2 {ECO:0000303|PubMed:23235153};
GN   OrderedLocusNames=pRL100451 {ECO:0000312|EMBL:CAK10677.1};
OS   Rhizobium leguminosarum bv. viciae (strain 3841).
OG   Plasmid pRL10 {ECO:0000312|EMBL:CAK10677.1}.
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales;
OC   Rhizobiaceae; Rhizobium/Agrobacterium group; Rhizobium.
OX   NCBI_TaxID=216596;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=3841;
RX   PubMed=16640791; DOI=10.1186/gb-2006-7-4-r34;
RA   Young J.P.W., Crossman L.C., Johnston A.W.B., Thomson N.R., Ghazoui Z.F.,
RA   Hull K.H., Wexler M., Curson A.R.J., Todd J.D., Poole P.S., Mauchline T.H.,
RA   East A.K., Quail M.A., Churcher C., Arrowsmith C., Cherevach I.,
RA   Chillingworth T., Clarke K., Cronin A., Davis P., Fraser A., Hance Z.,
RA   Hauser H., Jagels K., Moule S., Mungall K., Norbertczak H.,
RA   Rabbinowitsch E., Sanders M., Simmonds M., Whitehead S., Parkhill J.;
RT   "The genome of Rhizobium leguminosarum has recognizable core and accessory
RT   components.";
RL   Genome Biol. 7:R34.1-R34.20(2006).
RN   [2]
RP   IDENTIFICATION BY MASS SPECTROMETRY, AND SUBCELLULAR LOCATION.
RC   STRAIN=3841;
RX   PubMed=18230162; DOI=10.1186/1471-2164-9-55;
RA   Krehenbrink M., Downie J.A.;
RT   "Identification of protein secretion systems and novel secreted proteins in
RT   Rhizobium leguminosarum bv. viciae.";
RL   BMC Genomics 9:55-55(2008).
RN   [3]
RP   FUNCTION, AND DOMAIN.
RC   STRAIN=3841;
RX   PubMed=23235153; DOI=10.1074/jbc.m112.411769;
RA   Abdian P.L., Caramelo J.J., Ausmees N., Zorreguieta A.;
RT   "RapA2 is a calcium-binding lectin composed of two highly conserved
RT   cadherin-like domains that specifically recognize Rhizobium leguminosarum
RT   acidic exopolysaccharides.";
RL   J. Biol. Chem. 288:2893-2904(2013).
CC   -!- FUNCTION: Interacts specifically in a calcium-dependent manner with the
CC       acidic exopolysaccharide (EPS) and capsular polysaccharide produced by
CC       R.leguminosarum. Could be involved in the development of the biofilm
CC       matrix made of EPS. {ECO:0000269|PubMed:23235153}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:18230162}.
CC       Note=Secreted by the type I secretion system PrsDE.
CC       {ECO:0000269|PubMed:18230162}.
CC   -!- DOMAIN: Contains two Ra/cadherin-like domains composed mostly of beta-
CC       sheets joined by a linker region. Proper folding and stability are
CC       dependent on the binding of one calcium ion per protein molecule.
CC       {ECO:0000269|PubMed:23235153}.
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DR   EMBL; AM236084; CAK10677.1; -; Genomic_DNA.
DR   RefSeq; WP_011654474.1; NC_008381.1.
DR   AlphaFoldDB; Q1M755; -.
DR   EnsemblBacteria; CAK10677; CAK10677; pRL100451.
DR   KEGG; rle:pRL100451; -.
DR   HOGENOM; CLU_1194109_0_0_5; -.
DR   OMA; DSATFQE; -.
DR   OrthoDB; 1022912at2; -.
DR   Proteomes; UP000006575; Plasmid pRL10.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   InterPro; IPR040853; RapA2_cadherin-like.
DR   InterPro; IPR010221; VCBS_rpt.
DR   Pfam; PF17803; Cadherin_4; 2.
DR   TIGRFAMs; TIGR01965; VCBS_repeat; 2.
PE   1: Evidence at protein level;
KW   Calcium; Metal-binding; Plasmid; Secreted.
FT   CHAIN           1..236
FT                   /note="Calcium-binding lectin RapA2"
FT                   /id="PRO_0000435394"
SQ   SEQUENCE   236 AA;  24882 MW;  8C93FDAA8B4EFC15 CRC64;
     MASPIHATDD SATFQETDVI SGNLLSNDSS DNGHLFLRAF DGASVGAKSG NSQVTEIQGD
     YGTFFVKPDG SYTYVLSDAA KIGFANGESF QEKVSYKISD GSGHTDVGLF TLNIQGVTQV
     KPIAVDDHYS FNEGDAIGGN VLDNDIAGDN GHLFLRQFDG TNVSAKSGPD AVTDIVGDYG
     VFHVKPNGEF TYELTDDLAA GQTVTETVQY YKISDGEGHT DAGVLTLNIT GTDALV