RAP_ARATH
ID RAP_ARATH Reviewed; 671 AA.
AC Q8VZE7; A8W8T7; Q9SKB1;
DT 30-NOV-2016, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2002, sequence version 1.
DT 03-AUG-2022, entry version 115.
DE RecName: Full=RAP domain-containing protein, chloroplastic {ECO:0000305};
DE Short=AtRAP {ECO:0000303|PubMed:18003861};
DE Flags: Precursor;
GN Name=RAP {ECO:0000303|PubMed:18003861};
GN OrderedLocusNames=At2g31890 {ECO:0000312|Araport:AT2G31890};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617197; DOI=10.1038/45471;
RA Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D.,
RA Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V.,
RA Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S.,
RA Cronin L.A., Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J.,
RA Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M.,
RA Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O.,
RA Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.;
RT "Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana.";
RL Nature 402:761-768(1999).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [4]
RP NUCLEOTIDE SEQUENCE [MRNA] OF 623-671, FUNCTION, INDUCTION, AND DISRUPTION
RP PHENOTYPE.
RX PubMed=18003861; DOI=10.1101/gad.1595107;
RA Katiyar-Agarwal S., Gao S., Vivian-Smith A., Jin H.;
RT "A novel class of bacteria-induced small RNAs in Arabidopsis.";
RL Genes Dev. 21:3123-3134(2007).
RN [5]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=19376835; DOI=10.1104/pp.109.138677;
RA Reiland S., Messerli G., Baerenfaller K., Gerrits B., Endler A.,
RA Grossmann J., Gruissem W., Baginsky S.;
RT "Large-scale Arabidopsis phosphoproteome profiling reveals novel
RT chloroplast kinase substrates and phosphorylation networks.";
RL Plant Physiol. 150:889-903(2009).
RN [6]
RP FUNCTION, SUBCELLULAR LOCATION, AND DISRUPTION PHENOTYPE.
RX PubMed=24585838; DOI=10.1105/tpc.114.122853;
RA Kleinknecht L., Wang F., Stuebe R., Philippar K., Nickelsen J., Bohne A.V.;
RT "RAP, the sole octotricopeptide repeat protein in Arabidopsis, is required
RT for chloroplast 16S rRNA maturation.";
RL Plant Cell 26:777-787(2014).
CC -!- FUNCTION: RNA-binding protein required for chloroplast 16S rRNA
CC maturation, an important process which supports chloroplast gene
CC expression and biogenesis. Binds to 16S rRNA precursor and is involved
CC in its 5'-end processing (PubMed:24585838). May act as negative
CC regulator of defense response against bacterial pathogens
CC (PubMed:18003861). {ECO:0000269|PubMed:18003861,
CC ECO:0000269|PubMed:24585838}.
CC -!- SUBCELLULAR LOCATION: Plastid, chloroplast stroma, chloroplast nucleoid
CC {ECO:0000269|PubMed:24585838}.
CC -!- INDUCTION: Down-regulated during infection with the bacterial pathogen
CC Pseudomonas syringae pv. tomato avirulent avrRpt2 strain.
CC {ECO:0000269|PubMed:18003861}.
CC -!- DISRUPTION PHENOTYPE: Retarded growth and photobleaching phenotype
CC (PubMed:18003861, PubMed:24585838). Enhanced resistance to the
CC bacterial pathogen Pseudomonas syringae pv. tomato (PubMed:18003861).
CC {ECO:0000269|PubMed:18003861, ECO:0000269|PubMed:24585838}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAD32283.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AC006533; AAD32283.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002685; AEC08600.1; -; Genomic_DNA.
DR EMBL; AY065010; AAL57655.1; -; mRNA.
DR EMBL; AY129492; AAM91078.1; -; mRNA.
DR EMBL; EU199905; ABW77414.1; -; mRNA.
DR PIR; D84726; D84726.
DR RefSeq; NP_850176.1; NM_179845.4.
DR AlphaFoldDB; Q8VZE7; -.
DR SMR; Q8VZE7; -.
DR STRING; 3702.AT2G31890.1; -.
DR iPTMnet; Q8VZE7; -.
DR PaxDb; Q8VZE7; -.
DR PRIDE; Q8VZE7; -.
DR ProteomicsDB; 225919; -.
DR EnsemblPlants; AT2G31890.1; AT2G31890.1; AT2G31890.
DR GeneID; 817747; -.
DR Gramene; AT2G31890.1; AT2G31890.1; AT2G31890.
DR KEGG; ath:AT2G31890; -.
DR Araport; AT2G31890; -.
DR TAIR; locus:2045213; AT2G31890.
DR eggNOG; ENOG502QUHX; Eukaryota.
DR HOGENOM; CLU_028932_0_0_1; -.
DR InParanoid; Q8VZE7; -.
DR OMA; QMDRSFF; -.
DR OrthoDB; 558345at2759; -.
DR PhylomeDB; Q8VZE7; -.
DR PRO; PR:Q8VZE7; -.
DR Proteomes; UP000006548; Chromosome 2.
DR ExpressionAtlas; Q8VZE7; baseline and differential.
DR GO; GO:0009507; C:chloroplast; IBA:GO_Central.
DR GO; GO:0042644; C:chloroplast nucleoid; IEA:UniProtKB-SubCell.
DR GO; GO:0005759; C:mitochondrial matrix; IBA:GO_Central.
DR GO; GO:0042646; C:plastid nucleoid; IDA:TAIR.
DR GO; GO:0035770; C:ribonucleoprotein granule; IBA:GO_Central.
DR GO; GO:0003723; F:RNA binding; IDA:TAIR.
DR GO; GO:1901259; P:chloroplast rRNA processing; IMP:TAIR.
DR GO; GO:0006952; P:defense response; IEA:UniProtKB-KW.
DR GO; GO:0000963; P:mitochondrial RNA processing; IBA:GO_Central.
DR GO; GO:0044528; P:regulation of mitochondrial mRNA stability; IBA:GO_Central.
DR InterPro; IPR013584; RAP.
DR Pfam; PF08373; RAP; 1.
DR SMART; SM00952; RAP; 1.
DR PROSITE; PS51286; RAP; 1.
PE 1: Evidence at protein level;
KW Chloroplast; Plant defense; Plastid; Reference proteome; RNA-binding;
KW rRNA processing; Transit peptide.
FT TRANSIT 1..78
FT /note="Chloroplast"
FT /evidence="ECO:0000255"
FT CHAIN 79..671
FT /note="RAP domain-containing protein, chloroplastic"
FT /evidence="ECO:0000255"
FT /id="PRO_0000438500"
FT DOMAIN 607..665
FT /note="RAP"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00619"
SQ SEQUENCE 671 AA; 75679 MW; B8C7EF427044679F CRC64;
MECVVPFRRC FCLNPPETRH RIVNHNHRNL HISLSSSSFA SGILPLSNKK YRFVGPLAQR
SSLHRRTDSL KHLPFSVNAS VIGNSEEEVE EEDDDGDWEA EFLGEIDPLD IQPPKKRKKQ
KNSKALEDTE GMDWCVRARK IALKSIEARG LSSRMAEVMP LKKKKKKKSK KVIVKKDKVK
SKSIPEDDFD TEDEDLDFED GFVEDKMGDL RKRVSSLAGG MFEEKKEKMK EQLAQRLSQF
SGPSDRMKEI NLNKAIIEAQ TAEEVLEVTA ETIMAVAKGL SPSPLSPLNI ATALHRIAKN
MEKVSMMRTR RLAFARQREM SMLVALAMTC LPECSAQGIS NISWALSKIG GELLYLTEMD
RVAEVATSKV GEFNSQNVAN IAGAFASMRH SAPELFAELS KRASTIINTF KGQEIAQLLW
SFASLYEPAD PLLESLDSAF KSSDQFKCYL TKEITNSDEV VDAEVSDDVS RSPALSFNRD
QLGNIAWSYA VLGQVERPFF ANIWNTLTTL EEQRLSEQYR EDVMFASQVY LVNQCLKLEC
PHLQLSLCQE LEEKISRAGK TKRFNQKITS SFQKEVGRLL ISTGLDWAKE HDVDGYTVDV
ALVEKKVALE IDGPTHFSRN SGLPLGHTML KRRYVAAAGW KVVSLSLQEW EEHEGSHEQL
EYLREILTGC I
