RAS1_ARATH
ID RAS1_ARATH Reviewed; 230 AA.
AC O04515;
DT 13-NOV-2019, integrated into UniProtKB/Swiss-Prot.
DT 01-JUL-1997, sequence version 1.
DT 03-AUG-2022, entry version 134.
DE RecName: Full=Protein RESPONSE TO ABA AND SALT 1 {ECO:0000303|PubMed:20212128};
GN Name=RAS1 {ECO:0000303|PubMed:20212128};
GN OrderedLocusNames=At1g09950 {ECO:0000312|Araport:AT1G09950};
GN ORFNames=F21M12.34 {ECO:0000312|EMBL:AAB60750.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RA Cheuk R.F., Chen H., Kim C.J., Shinn P., Ecker J.R.;
RT "Arabidopsis ORF clones.";
RL Submitted (MAR-2004) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RA Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A.,
RA Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y.,
RA Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.,
RA Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y.,
RA Shinozaki K.;
RT "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs.";
RL Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP FUNCTION, DISRUPTION PHENOTYPE, INDUCTION BY ABSCISIC ACID AND SALT STRESS,
RP AND VARIANTS ARG-130 AND 210-LYS--ALA-230 DEL.
RC STRAIN=cv. Columbia, cv. Landsberg erecta, and cv. Sha;
RX PubMed=20212128; DOI=10.1073/pnas.0910798107;
RA Ren Z., Zheng Z., Chinnusamy V., Zhu J., Cui X., Iida K., Zhu J.-K.;
RT "RAS1, a quantitative trait locus for salt tolerance and ABA sensitivity in
RT Arabidopsis.";
RL Proc. Natl. Acad. Sci. U.S.A. 107:5669-5674(2010).
RN [6]
RP INDUCTION BY DROUGHT.
RX PubMed=28586434; DOI=10.1093/jxb/erx157;
RA Yin M., Wang Y., Zhang L., Li J., Quan W., Yang L., Wang Q., Chan Z.;
RT "The Arabidopsis Cys2/His2 zinc finger transcription factor ZAT18 is a
RT positive regulator of plant tolerance to drought stress.";
RL J. Exp. Bot. 68:2991-3005(2017).
CC -!- FUNCTION: Negative regulator of salt (NaCl) tolerance probably by
CC enhancing abscisic acid (ABA) sensitivity.
CC {ECO:0000269|PubMed:20212128}.
CC -!- INDUCTION: Induced by abscisic acid (ABA) and salt stress (NaCl)
CC (PubMed:20212128). Up-regulated by ZAT18 upon drought
CC (PubMed:28586434). {ECO:0000269|PubMed:20212128,
CC ECO:0000269|PubMed:28586434}.
CC -!- DISRUPTION PHENOTYPE: Decreased salt and abscisic acid (ABA)
CC sensitivity. {ECO:0000269|PubMed:20212128}.
CC -!- MISCELLANEOUS: Loss of function contributes to the increased salt
CC (NaCl) tolerance of cv. Sha. {ECO:0000269|PubMed:20212128}.
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DR EMBL; AC000132; AAB60750.1; -; Genomic_DNA.
DR EMBL; CP002684; AEE28520.1; -; Genomic_DNA.
DR EMBL; BT012212; AAS76699.1; -; mRNA.
DR EMBL; BT012400; AAS92316.1; -; mRNA.
DR EMBL; AK228972; BAF00861.1; -; mRNA.
DR PIR; H86233; H86233.
DR RefSeq; NP_172466.1; NM_100869.4.
DR AlphaFoldDB; O04515; -.
DR STRING; 3702.AT1G09950.1; -.
DR PaxDb; O04515; -.
DR PRIDE; O04515; -.
DR EnsemblPlants; AT1G09950.1; AT1G09950.1; AT1G09950.
DR GeneID; 837529; -.
DR Gramene; AT1G09950.1; AT1G09950.1; AT1G09950.
DR KEGG; ath:AT1G09950; -.
DR Araport; AT1G09950; -.
DR TAIR; locus:2024412; AT1G09950.
DR eggNOG; ENOG502RZPJ; Eukaryota.
DR HOGENOM; CLU_024782_2_0_1; -.
DR InParanoid; O04515; -.
DR OMA; NYSIGSE; -.
DR OrthoDB; 1404512at2759; -.
DR PhylomeDB; O04515; -.
DR PRO; PR:O04515; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; O04515; baseline and differential.
DR GO; GO:0043565; F:sequence-specific DNA binding; IEA:InterPro.
DR GO; GO:0009738; P:abscisic acid-activated signaling pathway; IEA:UniProtKB-KW.
DR GO; GO:0009788; P:negative regulation of abscisic acid-activated signaling pathway; IMP:UniProtKB.
DR GO; GO:1900070; P:negative regulation of cellular hyperosmotic salinity response; IMP:UniProtKB.
DR GO; GO:0009737; P:response to abscisic acid; IEP:UniProtKB.
DR GO; GO:0009651; P:response to salt stress; IEP:UniProtKB.
DR GO; GO:0006351; P:transcription, DNA-templated; IEA:InterPro.
DR InterPro; IPR025422; TGA_domain.
DR Pfam; PF14144; DOG1; 1.
DR PROSITE; PS51806; DOG1; 1.
PE 2: Evidence at transcript level;
KW Abscisic acid signaling pathway; Activator; Reference proteome;
KW Stress response; Transcription; Transcription regulation.
FT CHAIN 1..230
FT /note="Protein RESPONSE TO ABA AND SALT 1"
FT /id="PRO_0000448578"
FT DOMAIN 7..230
FT /note="DOG1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01147"
FT VARIANT 130
FT /note="K -> R (in strain: cv. Sha)"
FT /evidence="ECO:0000269|PubMed:20212128"
FT VARIANT 210..230
FT /note="Missing (in strain: cv. Sha; contributes to an
FT increased salt (NaCl) tolerance)"
FT /evidence="ECO:0000269|PubMed:20212128"
SQ SEQUENCE 230 AA; 26094 MW; 10B51D22FB98FB35 CRC64;
MPNTSSSQSF TIFVDGWLIR HRYFVEQLMC ASSLDETNRI SLEEQQSLVA QFLSHCLQYY
QEKFASVSLA GDNVFTFFCP PWFNSYAKLI LWVGDFKPSL VFKLTEVSVA DLTRHQKDRI
SSLKSETRRK EREVMRDFAL VQQSVADPPV MLAARRVGAV GMVDGEETDL EEAMEVLKAG
MAAAMNNADQ LRCSTVGKVV EILTPPQAIK VLRTIGQLHL RLRDRDQERA
