RAS1_DROPE
ID RAS1_DROPE Reviewed; 189 AA.
AC B4GFJ8;
DT 10-FEB-2009, integrated into UniProtKB/Swiss-Prot.
DT 23-SEP-2008, sequence version 1.
DT 03-AUG-2022, entry version 85.
DE RecName: Full=Ras-like protein 1 {ECO:0000250|UniProtKB:P08646};
DE EC=3.6.5.2 {ECO:0000250|UniProtKB:P01112};
DE Flags: Precursor;
GN Name=Ras85D {ECO:0000250|UniProtKB:P08646}; ORFNames=GL22227;
OS Drosophila persimilis (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7234;
RN [1] {ECO:0000312|EMBL:EDW34383.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=MSH-3 / Tucson 14011-0111.49;
RX PubMed=17994087; DOI=10.1038/nature06341;
RG Drosophila 12 genomes consortium;
RT "Evolution of genes and genomes on the Drosophila phylogeny.";
RL Nature 450:203-218(2007).
CC -!- FUNCTION: Ras proteins bind GDP/GTP and possess intrinsic GTPase
CC activity. Plays a role in eye development by regulating cell growth,
CC survival of postmitotic ommatidial cells and differentiation of
CC photoreceptor cells. During larval development, mediates Ptth/tor
CC signaling leading to the production of ecdysone, a hormone required for
CC the initiation of metamorphosis. {ECO:0000250|UniProtKB:P01112,
CC ECO:0000250|UniProtKB:P08646}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=GTP + H2O = GDP + H(+) + phosphate; Xref=Rhea:RHEA:19669,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:37565,
CC ChEBI:CHEBI:43474, ChEBI:CHEBI:58189; EC=3.6.5.2;
CC Evidence={ECO:0000250|UniProtKB:P01112};
CC -!- ACTIVITY REGULATION: Alternates between an inactive form bound to GDP
CC and an active form bound to GTP. Activated by a guanine nucleotide-
CC exchange factor (GEF) and inactivated by a GTPase-activating protein
CC (GAP).
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000250|UniProtKB:P08646};
CC Lipid-anchor {ECO:0000250|UniProtKB:P08646}; Cytoplasmic side
CC {ECO:0000250|UniProtKB:P08646}.
CC -!- SIMILARITY: Belongs to the small GTPase superfamily. Ras family.
CC {ECO:0000255}.
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DR EMBL; CH479182; EDW34383.1; -; Genomic_DNA.
DR RefSeq; XP_002017283.1; XM_002017247.1.
DR AlphaFoldDB; B4GFJ8; -.
DR SMR; B4GFJ8; -.
DR STRING; 7234.FBpp0186334; -.
DR EnsemblMetazoa; FBtr0187842; FBpp0186334; FBgn0159819.
DR GeneID; 6592147; -.
DR KEGG; dpe:6592147; -.
DR eggNOG; KOG0395; Eukaryota.
DR HOGENOM; CLU_041217_9_8_1; -.
DR OMA; CCSGCVV; -.
DR PhylomeDB; B4GFJ8; -.
DR ChiTaRS; Ras85D; fly.
DR Proteomes; UP000008744; Unassembled WGS sequence.
DR GO; GO:0016020; C:membrane; ISS:UniProtKB.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0003925; F:G protein activity; IEA:UniProtKB-EC.
DR GO; GO:0005525; F:GTP binding; IEA:UniProtKB-KW.
DR GO; GO:0043539; F:protein serine/threonine kinase activator activity; IEA:EnsemblMetazoa.
DR GO; GO:0007298; P:border follicle cell migration; IEA:EnsemblMetazoa.
DR GO; GO:0009267; P:cellular response to starvation; IEA:EnsemblMetazoa.
DR GO; GO:0030381; P:chorion-containing eggshell pattern formation; IEA:EnsemblMetazoa.
DR GO; GO:0051607; P:defense response to virus; IEA:EnsemblMetazoa.
DR GO; GO:0008340; P:determination of adult lifespan; IEA:EnsemblMetazoa.
DR GO; GO:0007395; P:dorsal closure, spreading of leading edge cells; IEA:EnsemblMetazoa.
DR GO; GO:0007173; P:epidermal growth factor receptor signaling pathway; IEA:EnsemblMetazoa.
DR GO; GO:0007427; P:epithelial cell migration, open tracheal system; IEA:EnsemblMetazoa.
DR GO; GO:0035088; P:establishment or maintenance of apical/basal cell polarity; IEA:EnsemblMetazoa.
DR GO; GO:0007455; P:eye-antennal disc morphogenesis; IEA:EnsemblMetazoa.
DR GO; GO:0008543; P:fibroblast growth factor receptor signaling pathway; IEA:EnsemblMetazoa.
DR GO; GO:0035099; P:hemocyte migration; IEA:EnsemblMetazoa.
DR GO; GO:0008586; P:imaginal disc-derived wing vein morphogenesis; IEA:EnsemblMetazoa.
DR GO; GO:0007474; P:imaginal disc-derived wing vein specification; IEA:EnsemblMetazoa.
DR GO; GO:0002168; P:instar larval development; IEA:EnsemblMetazoa.
DR GO; GO:0036335; P:intestinal stem cell homeostasis; IEA:EnsemblMetazoa.
DR GO; GO:0007479; P:leg disc proximal/distal pattern formation; IEA:EnsemblMetazoa.
DR GO; GO:0035170; P:lymph gland crystal cell differentiation; IEA:EnsemblMetazoa.
DR GO; GO:0035169; P:lymph gland plasmatocyte differentiation; IEA:EnsemblMetazoa.
DR GO; GO:0072002; P:Malpighian tubule development; IEA:EnsemblMetazoa.
DR GO; GO:0000165; P:MAPK cascade; IEA:EnsemblMetazoa.
DR GO; GO:0001710; P:mesodermal cell fate commitment; IEA:EnsemblMetazoa.
DR GO; GO:0048626; P:myoblast fate specification; IEA:EnsemblMetazoa.
DR GO; GO:2001234; P:negative regulation of apoptotic signaling pathway; IEA:EnsemblMetazoa.
DR GO; GO:0046673; P:negative regulation of compound eye retinal cell programmed cell death; IEA:EnsemblMetazoa.
DR GO; GO:0010629; P:negative regulation of gene expression; IEA:EnsemblMetazoa.
DR GO; GO:0016242; P:negative regulation of macroautophagy; IEA:EnsemblMetazoa.
DR GO; GO:0016318; P:ommatidial rotation; IEA:EnsemblMetazoa.
DR GO; GO:0007309; P:oocyte axis specification; IEA:EnsemblMetazoa.
DR GO; GO:0007422; P:peripheral nervous system development; IEA:EnsemblMetazoa.
DR GO; GO:0043703; P:photoreceptor cell fate determination; IEA:EnsemblMetazoa.
DR GO; GO:0008594; P:photoreceptor cell morphogenesis; IEA:EnsemblMetazoa.
DR GO; GO:0045793; P:positive regulation of cell size; IEA:EnsemblMetazoa.
DR GO; GO:0070374; P:positive regulation of ERK1 and ERK2 cascade; IEA:EnsemblMetazoa.
DR GO; GO:0035208; P:positive regulation of hemocyte proliferation; IEA:EnsemblMetazoa.
DR GO; GO:0046534; P:positive regulation of photoreceptor cell differentiation; IEA:EnsemblMetazoa.
DR GO; GO:1904263; P:positive regulation of TORC1 signaling; IEA:EnsemblMetazoa.
DR GO; GO:0007265; P:Ras protein signal transduction; IEA:EnsemblMetazoa.
DR GO; GO:0040014; P:regulation of multicellular organism growth; IEA:EnsemblMetazoa.
DR GO; GO:0045500; P:sevenless signaling pathway; IEA:EnsemblMetazoa.
DR GO; GO:0048865; P:stem cell fate commitment; IEA:EnsemblMetazoa.
DR GO; GO:0072089; P:stem cell proliferation; IEA:EnsemblMetazoa.
DR GO; GO:0007430; P:terminal branching, open tracheal system; IEA:EnsemblMetazoa.
DR GO; GO:0007362; P:terminal region determination; IEA:EnsemblMetazoa.
DR GO; GO:0008293; P:torso signaling pathway; IEA:EnsemblMetazoa.
DR GO; GO:0060438; P:trachea development; IEA:EnsemblMetazoa.
DR GO; GO:0007426; P:tracheal outgrowth, open tracheal system; IEA:EnsemblMetazoa.
DR GO; GO:0048010; P:vascular endothelial growth factor receptor signaling pathway; IEA:EnsemblMetazoa.
DR Gene3D; 3.40.50.300; -; 1.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR005225; Small_GTP-bd_dom.
DR InterPro; IPR001806; Small_GTPase.
DR InterPro; IPR020849; Small_GTPase_Ras-type.
DR PANTHER; PTHR24070; PTHR24070; 1.
DR Pfam; PF00071; Ras; 1.
DR SMART; SM00174; RHO; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
DR TIGRFAMs; TIGR00231; small_GTP; 1.
DR PROSITE; PS51421; RAS; 1.
PE 3: Inferred from homology;
KW Cell membrane; GTP-binding; Hydrolase; Lipoprotein; Membrane; Methylation;
KW Nucleotide-binding; Prenylation; Reference proteome.
FT CHAIN 1..186
FT /note="Ras-like protein 1"
FT /evidence="ECO:0000250|UniProtKB:P08646"
FT /id="PRO_0000363714"
FT PROPEP 187..189
FT /note="Removed in mature form"
FT /evidence="ECO:0000250|UniProtKB:P08646"
FT /id="PRO_0000363715"
FT MOTIF 32..40
FT /note="Effector region"
FT BINDING 10..17
FT /ligand="GTP"
FT /ligand_id="ChEBI:CHEBI:37565"
FT /evidence="ECO:0000250|UniProtKB:P01112"
FT BINDING 57..61
FT /ligand="GTP"
FT /ligand_id="ChEBI:CHEBI:37565"
FT /evidence="ECO:0000250|UniProtKB:P01112"
FT BINDING 116..119
FT /ligand="GTP"
FT /ligand_id="ChEBI:CHEBI:37565"
FT /evidence="ECO:0000250|UniProtKB:P01112"
FT MOD_RES 186
FT /note="Cysteine methyl ester"
FT /evidence="ECO:0000250|UniProtKB:P08646"
FT LIPID 186
FT /note="S-geranylgeranyl cysteine"
FT /evidence="ECO:0000250|UniProtKB:P08646"
SQ SEQUENCE 189 AA; 21616 MW; 5954AABE1CD3B8DF CRC64;
MTEYKLVVVG AGGVGKSALT IQLIQNHFVD EYDPTIEDSY RKQVVIDGET CLLDILDTAG
QEEYSAMRDQ YMRTGEGFLL VFAVNSAKSF EDIGTYREQI KRVKDAEEVP MVLVGNKCDL
TTWNVKNEQA REVAKQYGIP YIETSAKTRM GVDDAFYTLV REIRKDKDNK GRRGRKLNKP
NRRFKCKIL
