RAS1_DROPS
ID RAS1_DROPS Reviewed; 189 AA.
AC Q295X7;
DT 10-FEB-2009, integrated into UniProtKB/Swiss-Prot.
DT 04-APR-2006, sequence version 1.
DT 03-AUG-2022, entry version 107.
DE RecName: Full=Ras-like protein 1 {ECO:0000250|UniProtKB:P08646};
DE EC=3.6.5.2 {ECO:0000250|UniProtKB:P01112};
DE Flags: Precursor;
GN Name=Ras85D {ECO:0000250|UniProtKB:P08646}; ORFNames=GA21740;
OS Drosophila pseudoobscura pseudoobscura (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=46245;
RN [1] {ECO:0000312|EMBL:EAL28581.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=MV2-25 / Tucson 14011-0121.94;
RX PubMed=15632085; DOI=10.1101/gr.3059305;
RA Richards S., Liu Y., Bettencourt B.R., Hradecky P., Letovsky S.,
RA Nielsen R., Thornton K., Hubisz M.J., Chen R., Meisel R.P., Couronne O.,
RA Hua S., Smith M.A., Zhang P., Liu J., Bussemaker H.J., van Batenburg M.F.,
RA Howells S.L., Scherer S.E., Sodergren E., Matthews B.B., Crosby M.A.,
RA Schroeder A.J., Ortiz-Barrientos D., Rives C.M., Metzker M.L., Muzny D.M.,
RA Scott G., Steffen D., Wheeler D.A., Worley K.C., Havlak P., Durbin K.J.,
RA Egan A., Gill R., Hume J., Morgan M.B., Miner G., Hamilton C., Huang Y.,
RA Waldron L., Verduzco D., Clerc-Blankenburg K.P., Dubchak I., Noor M.A.F.,
RA Anderson W., White K.P., Clark A.G., Schaeffer S.W., Gelbart W.M.,
RA Weinstock G.M., Gibbs R.A.;
RT "Comparative genome sequencing of Drosophila pseudoobscura: chromosomal,
RT gene, and cis-element evolution.";
RL Genome Res. 15:1-18(2005).
CC -!- FUNCTION: Ras proteins bind GDP/GTP and possess intrinsic GTPase
CC activity. Plays a role in eye development by regulating cell growth,
CC survival of postmitotic ommatidial cells and differentiation of
CC photoreceptor cells. During larval development, mediates Ptth/tor
CC signaling leading to the production of ecdysone, a hormone required for
CC the initiation of metamorphosis. {ECO:0000250|UniProtKB:P01112,
CC ECO:0000250|UniProtKB:P08646}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=GTP + H2O = GDP + H(+) + phosphate; Xref=Rhea:RHEA:19669,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:37565,
CC ChEBI:CHEBI:43474, ChEBI:CHEBI:58189; EC=3.6.5.2;
CC Evidence={ECO:0000250|UniProtKB:P01112};
CC -!- ACTIVITY REGULATION: Alternates between an inactive form bound to GDP
CC and an active form bound to GTP. Activated by a guanine nucleotide-
CC exchange factor (GEF) and inactivated by a GTPase-activating protein
CC (GAP).
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000250|UniProtKB:P08646};
CC Lipid-anchor {ECO:0000250|UniProtKB:P08646}; Cytoplasmic side
CC {ECO:0000250|UniProtKB:P08646}.
CC -!- SIMILARITY: Belongs to the small GTPase superfamily. Ras family.
CC {ECO:0000255}.
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DR EMBL; CM000070; EAL28581.1; -; Genomic_DNA.
DR RefSeq; XP_001359435.1; XM_001359398.3.
DR AlphaFoldDB; Q295X7; -.
DR SMR; Q295X7; -.
DR STRING; 7237.FBpp0284490; -.
DR EnsemblMetazoa; FBtr0286052; FBpp0284490; FBgn0081725.
DR GeneID; 4802534; -.
DR KEGG; dpo:Dpse_GA21740; -.
DR eggNOG; KOG0395; Eukaryota.
DR HOGENOM; CLU_041217_9_8_1; -.
DR InParanoid; Q295X7; -.
DR OMA; CCSGCVV; -.
DR PhylomeDB; Q295X7; -.
DR ChiTaRS; Ras85D; fly.
DR Proteomes; UP000001819; Chromosome 2.
DR Bgee; FBgn0081725; Expressed in female reproductive system and 2 other tissues.
DR GO; GO:0016020; C:membrane; ISS:UniProtKB.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0003925; F:G protein activity; IEA:UniProtKB-EC.
DR GO; GO:0005525; F:GTP binding; IEA:UniProtKB-KW.
DR GO; GO:0043539; F:protein serine/threonine kinase activator activity; IEA:EnsemblMetazoa.
DR GO; GO:0007298; P:border follicle cell migration; IEA:EnsemblMetazoa.
DR GO; GO:0009267; P:cellular response to starvation; IEA:EnsemblMetazoa.
DR GO; GO:0030381; P:chorion-containing eggshell pattern formation; IEA:EnsemblMetazoa.
DR GO; GO:0051607; P:defense response to virus; IEA:EnsemblMetazoa.
DR GO; GO:0008340; P:determination of adult lifespan; IEA:EnsemblMetazoa.
DR GO; GO:0007395; P:dorsal closure, spreading of leading edge cells; IEA:EnsemblMetazoa.
DR GO; GO:0007173; P:epidermal growth factor receptor signaling pathway; IEA:EnsemblMetazoa.
DR GO; GO:0007427; P:epithelial cell migration, open tracheal system; IEA:EnsemblMetazoa.
DR GO; GO:0035088; P:establishment or maintenance of apical/basal cell polarity; IEA:EnsemblMetazoa.
DR GO; GO:0007455; P:eye-antennal disc morphogenesis; IEA:EnsemblMetazoa.
DR GO; GO:0008543; P:fibroblast growth factor receptor signaling pathway; IEA:EnsemblMetazoa.
DR GO; GO:0035099; P:hemocyte migration; IEA:EnsemblMetazoa.
DR GO; GO:0008586; P:imaginal disc-derived wing vein morphogenesis; IEA:EnsemblMetazoa.
DR GO; GO:0007474; P:imaginal disc-derived wing vein specification; IEA:EnsemblMetazoa.
DR GO; GO:0002168; P:instar larval development; IEA:EnsemblMetazoa.
DR GO; GO:0036335; P:intestinal stem cell homeostasis; IEA:EnsemblMetazoa.
DR GO; GO:0007479; P:leg disc proximal/distal pattern formation; IEA:EnsemblMetazoa.
DR GO; GO:0035170; P:lymph gland crystal cell differentiation; IEA:EnsemblMetazoa.
DR GO; GO:0035169; P:lymph gland plasmatocyte differentiation; IEA:EnsemblMetazoa.
DR GO; GO:0072002; P:Malpighian tubule development; IEA:EnsemblMetazoa.
DR GO; GO:0000165; P:MAPK cascade; IEA:EnsemblMetazoa.
DR GO; GO:0001710; P:mesodermal cell fate commitment; IEA:EnsemblMetazoa.
DR GO; GO:0048626; P:myoblast fate specification; IEA:EnsemblMetazoa.
DR GO; GO:2001234; P:negative regulation of apoptotic signaling pathway; IEA:EnsemblMetazoa.
DR GO; GO:0046673; P:negative regulation of compound eye retinal cell programmed cell death; IEA:EnsemblMetazoa.
DR GO; GO:0010629; P:negative regulation of gene expression; IEA:EnsemblMetazoa.
DR GO; GO:0016242; P:negative regulation of macroautophagy; IEA:EnsemblMetazoa.
DR GO; GO:0016318; P:ommatidial rotation; IEA:EnsemblMetazoa.
DR GO; GO:0007309; P:oocyte axis specification; IEA:EnsemblMetazoa.
DR GO; GO:0007422; P:peripheral nervous system development; IEA:EnsemblMetazoa.
DR GO; GO:0043703; P:photoreceptor cell fate determination; IEA:EnsemblMetazoa.
DR GO; GO:0008594; P:photoreceptor cell morphogenesis; IEA:EnsemblMetazoa.
DR GO; GO:0045793; P:positive regulation of cell size; IEA:EnsemblMetazoa.
DR GO; GO:0070374; P:positive regulation of ERK1 and ERK2 cascade; IEA:EnsemblMetazoa.
DR GO; GO:0035208; P:positive regulation of hemocyte proliferation; IEA:EnsemblMetazoa.
DR GO; GO:0046534; P:positive regulation of photoreceptor cell differentiation; IEA:EnsemblMetazoa.
DR GO; GO:1904263; P:positive regulation of TORC1 signaling; IEA:EnsemblMetazoa.
DR GO; GO:0007265; P:Ras protein signal transduction; IEA:EnsemblMetazoa.
DR GO; GO:0040014; P:regulation of multicellular organism growth; IEA:EnsemblMetazoa.
DR GO; GO:0045500; P:sevenless signaling pathway; IEA:EnsemblMetazoa.
DR GO; GO:0048865; P:stem cell fate commitment; IEA:EnsemblMetazoa.
DR GO; GO:0072089; P:stem cell proliferation; IEA:EnsemblMetazoa.
DR GO; GO:0007430; P:terminal branching, open tracheal system; IEA:EnsemblMetazoa.
DR GO; GO:0007362; P:terminal region determination; IEA:EnsemblMetazoa.
DR GO; GO:0008293; P:torso signaling pathway; IEA:EnsemblMetazoa.
DR GO; GO:0060438; P:trachea development; IEA:EnsemblMetazoa.
DR GO; GO:0007426; P:tracheal outgrowth, open tracheal system; IEA:EnsemblMetazoa.
DR GO; GO:0048010; P:vascular endothelial growth factor receptor signaling pathway; IEA:EnsemblMetazoa.
DR Gene3D; 3.40.50.300; -; 1.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR005225; Small_GTP-bd_dom.
DR InterPro; IPR001806; Small_GTPase.
DR InterPro; IPR020849; Small_GTPase_Ras-type.
DR PANTHER; PTHR24070; PTHR24070; 1.
DR Pfam; PF00071; Ras; 1.
DR SMART; SM00174; RHO; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
DR TIGRFAMs; TIGR00231; small_GTP; 1.
DR PROSITE; PS51421; RAS; 1.
PE 3: Inferred from homology;
KW Cell membrane; GTP-binding; Hydrolase; Lipoprotein; Membrane; Methylation;
KW Nucleotide-binding; Prenylation; Reference proteome.
FT CHAIN 1..186
FT /note="Ras-like protein 1"
FT /evidence="ECO:0000250|UniProtKB:P08646"
FT /id="PRO_0000363716"
FT PROPEP 187..189
FT /note="Removed in mature form"
FT /evidence="ECO:0000250|UniProtKB:P08646"
FT /id="PRO_0000363717"
FT MOTIF 32..40
FT /note="Effector region"
FT BINDING 10..17
FT /ligand="GTP"
FT /ligand_id="ChEBI:CHEBI:37565"
FT /evidence="ECO:0000250|UniProtKB:P01112"
FT BINDING 57..61
FT /ligand="GTP"
FT /ligand_id="ChEBI:CHEBI:37565"
FT /evidence="ECO:0000250|UniProtKB:P01112"
FT BINDING 116..119
FT /ligand="GTP"
FT /ligand_id="ChEBI:CHEBI:37565"
FT /evidence="ECO:0000250|UniProtKB:P01112"
FT MOD_RES 186
FT /note="Cysteine methyl ester"
FT /evidence="ECO:0000250|UniProtKB:P08646"
FT LIPID 186
FT /note="S-geranylgeranyl cysteine"
FT /evidence="ECO:0000250|UniProtKB:P08646"
SQ SEQUENCE 189 AA; 21616 MW; 5954AABE1CD3B8DF CRC64;
MTEYKLVVVG AGGVGKSALT IQLIQNHFVD EYDPTIEDSY RKQVVIDGET CLLDILDTAG
QEEYSAMRDQ YMRTGEGFLL VFAVNSAKSF EDIGTYREQI KRVKDAEEVP MVLVGNKCDL
TTWNVKNEQA REVAKQYGIP YIETSAKTRM GVDDAFYTLV REIRKDKDNK GRRGRKLNKP
NRRFKCKIL
