RAS1_DROSI
ID RAS1_DROSI Reviewed; 189 AA.
AC P83831; B4QWL7;
DT 29-MAR-2004, integrated into UniProtKB/Swiss-Prot.
DT 15-MAR-2004, sequence version 1.
DT 03-AUG-2022, entry version 118.
DE RecName: Full=Ras-like protein 1;
DE EC=3.6.5.2 {ECO:0000250|UniProtKB:P01112};
DE Flags: Precursor;
GN Name=Ras85D; Synonyms=Ras1; ORFNames=GD20791;
OS Drosophila simulans (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7240 {ECO:0000312|EMBL:AAF15515.1};
RN [1] {ECO:0000305}
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=10552039; DOI=10.1007/pl00006579;
RA Gasperini R., Gibson G.;
RT "Absence of protein polymorphism in the Ras genes of Drosophila
RT melanogaster.";
RL J. Mol. Evol. 49:583-590(1999).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=17994087; DOI=10.1038/nature06341;
RG Drosophila 12 genomes consortium;
RT "Evolution of genes and genomes on the Drosophila phylogeny.";
RL Nature 450:203-218(2007).
RN [3] {ECO:0000305}
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-18.
RX PubMed=14762063; DOI=10.1101/gr.1329204;
RA Halligan D.L., Eyre-Walker A., Andolfatto P., Keightley P.D.;
RT "Patterns of evolutionary constraints in intronic and intergenic DNA of
RT Drosophila.";
RL Genome Res. 14:273-279(2004).
CC -!- FUNCTION: Ras proteins bind GDP/GTP and possess intrinsic GTPase
CC activity. Plays a role in eye development by regulating cell growth,
CC survival of postmitotic ommatidial cells and differentiation of
CC photoreceptor cells. During larval development, mediates Ptth/tor
CC signaling leading to the production of ecdysone, a hormone required for
CC the initiation of metamorphosis. {ECO:0000250|UniProtKB:P01112,
CC ECO:0000250|UniProtKB:P08646}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=GTP + H2O = GDP + H(+) + phosphate; Xref=Rhea:RHEA:19669,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:37565,
CC ChEBI:CHEBI:43474, ChEBI:CHEBI:58189; EC=3.6.5.2;
CC Evidence={ECO:0000250|UniProtKB:P01112};
CC -!- ACTIVITY REGULATION: Alternates between an inactive form bound to GDP
CC and an active form bound to GTP. Activated by a guanine nucleotide-
CC exchange factor (GEF) and inactivated by a GTPase-activating protein
CC (GAP).
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000250}; Lipid-anchor
CC {ECO:0000250}; Cytoplasmic side {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the small GTPase superfamily. Ras family.
CC {ECO:0000305}.
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DR EMBL; AF186649; AAF15515.1; -; Genomic_DNA.
DR EMBL; CM000364; EDX13621.1; -; Genomic_DNA.
DR EMBL; AY459550; AAR23008.1; -; Genomic_DNA.
DR RefSeq; XP_016035463.1; XM_016177119.1.
DR AlphaFoldDB; P83831; -.
DR SMR; P83831; -.
DR STRING; 7240.P83831; -.
DR EnsemblMetazoa; FBtr0220701; FBpp0219193; FBgn0029246.
DR GeneID; 6728783; -.
DR HOGENOM; CLU_041217_9_8_1; -.
DR OMA; SSRRFKC; -.
DR PhylomeDB; P83831; -.
DR ChiTaRS; Ras85D; fly.
DR Proteomes; UP000000304; Chromosome 3r.
DR Bgee; FBgn0029246; Expressed in embryo and 3 other tissues.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0003925; F:G protein activity; IEA:UniProtKB-EC.
DR GO; GO:0005525; F:GTP binding; IEA:UniProtKB-KW.
DR GO; GO:0043539; F:protein serine/threonine kinase activator activity; IEA:EnsemblMetazoa.
DR GO; GO:0007298; P:border follicle cell migration; IEA:EnsemblMetazoa.
DR GO; GO:0009267; P:cellular response to starvation; IEA:EnsemblMetazoa.
DR GO; GO:0030381; P:chorion-containing eggshell pattern formation; IEA:EnsemblMetazoa.
DR GO; GO:0051607; P:defense response to virus; IEA:EnsemblMetazoa.
DR GO; GO:0008340; P:determination of adult lifespan; IEA:EnsemblMetazoa.
DR GO; GO:0007395; P:dorsal closure, spreading of leading edge cells; IEA:EnsemblMetazoa.
DR GO; GO:0007173; P:epidermal growth factor receptor signaling pathway; IEA:EnsemblMetazoa.
DR GO; GO:0007427; P:epithelial cell migration, open tracheal system; IEA:EnsemblMetazoa.
DR GO; GO:0035088; P:establishment or maintenance of apical/basal cell polarity; IEA:EnsemblMetazoa.
DR GO; GO:0007455; P:eye-antennal disc morphogenesis; IEA:EnsemblMetazoa.
DR GO; GO:0008543; P:fibroblast growth factor receptor signaling pathway; IEA:EnsemblMetazoa.
DR GO; GO:0035099; P:hemocyte migration; IEA:EnsemblMetazoa.
DR GO; GO:0008586; P:imaginal disc-derived wing vein morphogenesis; IEA:EnsemblMetazoa.
DR GO; GO:0007474; P:imaginal disc-derived wing vein specification; IEA:EnsemblMetazoa.
DR GO; GO:0002168; P:instar larval development; IEA:EnsemblMetazoa.
DR GO; GO:0036335; P:intestinal stem cell homeostasis; IEA:EnsemblMetazoa.
DR GO; GO:0007479; P:leg disc proximal/distal pattern formation; IEA:EnsemblMetazoa.
DR GO; GO:0035170; P:lymph gland crystal cell differentiation; IEA:EnsemblMetazoa.
DR GO; GO:0035169; P:lymph gland plasmatocyte differentiation; IEA:EnsemblMetazoa.
DR GO; GO:0072002; P:Malpighian tubule development; IEA:EnsemblMetazoa.
DR GO; GO:0000165; P:MAPK cascade; IEA:EnsemblMetazoa.
DR GO; GO:0001710; P:mesodermal cell fate commitment; IEA:EnsemblMetazoa.
DR GO; GO:0048626; P:myoblast fate specification; IEA:EnsemblMetazoa.
DR GO; GO:2001234; P:negative regulation of apoptotic signaling pathway; IEA:EnsemblMetazoa.
DR GO; GO:0046673; P:negative regulation of compound eye retinal cell programmed cell death; IEA:EnsemblMetazoa.
DR GO; GO:0010629; P:negative regulation of gene expression; IEA:EnsemblMetazoa.
DR GO; GO:0016242; P:negative regulation of macroautophagy; IEA:EnsemblMetazoa.
DR GO; GO:0016318; P:ommatidial rotation; IEA:EnsemblMetazoa.
DR GO; GO:0007309; P:oocyte axis specification; IEA:EnsemblMetazoa.
DR GO; GO:0007422; P:peripheral nervous system development; IEA:EnsemblMetazoa.
DR GO; GO:0043703; P:photoreceptor cell fate determination; IEA:EnsemblMetazoa.
DR GO; GO:0008594; P:photoreceptor cell morphogenesis; IEA:EnsemblMetazoa.
DR GO; GO:0045793; P:positive regulation of cell size; IEA:EnsemblMetazoa.
DR GO; GO:0070374; P:positive regulation of ERK1 and ERK2 cascade; IEA:EnsemblMetazoa.
DR GO; GO:0035208; P:positive regulation of hemocyte proliferation; IEA:EnsemblMetazoa.
DR GO; GO:0046534; P:positive regulation of photoreceptor cell differentiation; IEA:EnsemblMetazoa.
DR GO; GO:1904263; P:positive regulation of TORC1 signaling; IEA:EnsemblMetazoa.
DR GO; GO:0007265; P:Ras protein signal transduction; IEA:EnsemblMetazoa.
DR GO; GO:0040014; P:regulation of multicellular organism growth; IEA:EnsemblMetazoa.
DR GO; GO:0045500; P:sevenless signaling pathway; IEA:EnsemblMetazoa.
DR GO; GO:0048865; P:stem cell fate commitment; IEA:EnsemblMetazoa.
DR GO; GO:0072089; P:stem cell proliferation; IEA:EnsemblMetazoa.
DR GO; GO:0007430; P:terminal branching, open tracheal system; IEA:EnsemblMetazoa.
DR GO; GO:0007362; P:terminal region determination; IEA:EnsemblMetazoa.
DR GO; GO:0008293; P:torso signaling pathway; IEA:EnsemblMetazoa.
DR GO; GO:0060438; P:trachea development; IEA:EnsemblMetazoa.
DR GO; GO:0007426; P:tracheal outgrowth, open tracheal system; IEA:EnsemblMetazoa.
DR GO; GO:0048010; P:vascular endothelial growth factor receptor signaling pathway; IEA:EnsemblMetazoa.
DR Gene3D; 3.40.50.300; -; 1.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR005225; Small_GTP-bd_dom.
DR InterPro; IPR001806; Small_GTPase.
DR InterPro; IPR020849; Small_GTPase_Ras-type.
DR PANTHER; PTHR24070; PTHR24070; 1.
DR Pfam; PF00071; Ras; 1.
DR SMART; SM00174; RHO; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
DR TIGRFAMs; TIGR00231; small_GTP; 1.
DR PROSITE; PS51421; RAS; 1.
PE 3: Inferred from homology;
KW Cell membrane; GTP-binding; Hydrolase; Lipoprotein; Membrane; Methylation;
KW Nucleotide-binding; Prenylation; Reference proteome.
FT CHAIN 1..186
FT /note="Ras-like protein 1"
FT /id="PRO_0000082667"
FT PROPEP 187..189
FT /note="Removed in mature form"
FT /evidence="ECO:0000250"
FT /id="PRO_0000281315"
FT MOTIF 32..40
FT /note="Effector region"
FT BINDING 10..17
FT /ligand="GTP"
FT /ligand_id="ChEBI:CHEBI:37565"
FT /evidence="ECO:0000250|UniProtKB:P08646"
FT BINDING 57..61
FT /ligand="GTP"
FT /ligand_id="ChEBI:CHEBI:37565"
FT /evidence="ECO:0000250|UniProtKB:P08646"
FT BINDING 116..119
FT /ligand="GTP"
FT /ligand_id="ChEBI:CHEBI:37565"
FT /evidence="ECO:0000250|UniProtKB:P08646"
FT MOD_RES 186
FT /note="Cysteine methyl ester"
FT /evidence="ECO:0000250"
FT LIPID 186
FT /note="S-geranylgeranyl cysteine"
FT /evidence="ECO:0000250|UniProtKB:P08646"
SQ SEQUENCE 189 AA; 21594 MW; 14236DCD65EACCD2 CRC64;
MTEYKLVVVG AGGVGKSALT IQLIQNHFVD EYDPTIEDSY RKQVVIDGET CLLDILDTAG
QEEYSAMRDQ YMRTGEGFLL VFAVNSAKSF EDIGTYREQI KRVKDAEEVP MVLVGNKCDL
ASWNVNNEQA REVAKQYGIP YIETSAKTRM GVDDAFYTLV REIRKDKDNK GRRGRKMNKP
NRRFKCKML
