RASEF_CAEEL
ID RASEF_CAEEL Reviewed; 634 AA.
AC Q22908; Q21466; Q22906;
DT 11-SEP-2007, integrated into UniProtKB/Swiss-Prot.
DT 07-APR-2021, sequence version 3.
DT 03-AUG-2022, entry version 156.
DE RecName: Full=Ras and EF-hand domain-containing protein homolog;
DE Flags: Precursor;
GN Name=rsef-1 {ECO:0000312|WormBase:C33D12.6};
GN Synonyms=tag-312 {ECO:0000312|WormBase:C33D12.6};
GN ORFNames=C33D12.6 {ECO:0000312|WormBase:C33D12.6};
OS Caenorhabditis elegans.
OC Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
OC Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae;
OC Caenorhabditis.
OX NCBI_TaxID=6239;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Bristol N2;
RX PubMed=9851916; DOI=10.1126/science.282.5396.2012;
RG The C. elegans sequencing consortium;
RT "Genome sequence of the nematode C. elegans: a platform for investigating
RT biology.";
RL Science 282:2012-2018(1998).
RN [2]
RP FUNCTION, DEVELOPMENTAL STAGE, AND DISRUPTION PHENOTYPE.
RX PubMed=25281934; DOI=10.1016/j.ydbio.2014.09.028;
RA Ghosh S., Sternberg P.W.;
RT "Spatial and molecular cues for cell outgrowth during C. elegans uterine
RT development.";
RL Dev. Biol. 396:121-135(2014).
CC -!- FUNCTION: Binds GTP and GDP (By similarity). Plays a role in uterine
CC seam cell development (PubMed:25281934). {ECO:0000250|UniProtKB:Q8IZ41,
CC ECO:0000269|PubMed:25281934}.
CC -!- SUBUNIT: Homodimer. {ECO:0000250|UniProtKB:Q8IZ41}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm, perinuclear region
CC {ECO:0000250|UniProtKB:Q8IZ41}.
CC -!- DEVELOPMENTAL STAGE: Expressed in the spermatheca and spermathecal-
CC uterine junction at the L4 lethargus stage of larval development and in
CC uterine toroid cells of young adults. Not expressed during early and
CC mid-L4 stages of larval development. {ECO:0000269|PubMed:25281934}.
CC -!- DISRUPTION PHENOTYPE: RNAi-mediated knockdown results in defective
CC uterine seam (utse) cell nuclear migration, defective utse cell
CC outgrowth and irregular uterine lumen formation.
CC {ECO:0000269|PubMed:25281934}.
CC -!- SIMILARITY: Belongs to the small GTPase superfamily. Rab family.
CC {ECO:0000305}.
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DR EMBL; BX284606; CCD66473.2; -; Genomic_DNA.
DR PIR; T16628; T16628.
DR PIR; T25607; T25607.
DR RefSeq; NP_508523.1; NM_076122.3.
DR AlphaFoldDB; Q22908; -.
DR SMR; Q22908; -.
DR STRING; 6239.C33D12.6; -.
DR PaxDb; Q22908; -.
DR PRIDE; Q22908; -.
DR EnsemblMetazoa; C33D12.6.1; C33D12.6.1; WBGene00016344.
DR UCSC; C33D12.6; c. elegans.
DR WormBase; C33D12.6; CE53320; WBGene00016344; rsef-1.
DR eggNOG; KOG0078; Eukaryota.
DR GeneTree; ENSGT00940000167998; -.
DR HOGENOM; CLU_023178_1_0_1; -.
DR InParanoid; Q22908; -.
DR OrthoDB; 1184845at2759; -.
DR PhylomeDB; Q22908; -.
DR Reactome; R-CEL-6798695; Neutrophil degranulation.
DR Reactome; R-CEL-8873719; RAB geranylgeranylation.
DR PRO; PR:Q22908; -.
DR Proteomes; UP000001940; Chromosome X.
DR Bgee; WBGene00016344; Expressed in pharyngeal muscle cell (C elegans) and 3 other tissues.
DR GO; GO:0048471; C:perinuclear region of cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0005509; F:calcium ion binding; IEA:InterPro.
DR GO; GO:0005525; F:GTP binding; IEA:UniProtKB-KW.
DR GO; GO:0003924; F:GTPase activity; IEA:InterPro.
DR GO; GO:0016192; P:vesicle-mediated transport; IBA:GO_Central.
DR Gene3D; 3.40.50.300; -; 1.
DR InterPro; IPR011992; EF-hand-dom_pair.
DR InterPro; IPR018247; EF_Hand_1_Ca_BS.
DR InterPro; IPR002048; EF_hand_dom.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR005225; Small_GTP-bd_dom.
DR InterPro; IPR001806; Small_GTPase.
DR Pfam; PF00071; Ras; 1.
DR SMART; SM00054; EFh; 2.
DR SMART; SM00174; RHO; 1.
DR SUPFAM; SSF47473; SSF47473; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
DR TIGRFAMs; TIGR00231; small_GTP; 1.
DR PROSITE; PS00018; EF_HAND_1; 1.
DR PROSITE; PS50222; EF_HAND_2; 1.
DR PROSITE; PS51419; RAB; 1.
PE 2: Evidence at transcript level;
KW Calcium; Coiled coil; Cytoplasm; GTP-binding; Metal-binding;
KW Nucleotide-binding; Reference proteome; Repeat.
FT CHAIN 1..631
FT /note="Ras and EF-hand domain-containing protein homolog"
FT /id="PRO_0000299582"
FT PROPEP 632..634
FT /note="Removed in mature form"
FT /evidence="ECO:0000255"
FT /id="PRO_0000370842"
FT DOMAIN 5..33
FT /note="EF-hand 1"
FT /evidence="ECO:0000305"
FT DOMAIN 33..68
FT /note="EF-hand 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT REGION 212..234
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 169..310
FT /evidence="ECO:0000255"
FT BINDING 46
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 48
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 50
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 52
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 57
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 449..454
FT /ligand="GTP"
FT /ligand_id="ChEBI:CHEBI:37565"
FT /evidence="ECO:0000250|UniProtKB:Q8IZ41"
FT BINDING 552..555
FT /ligand="GTP"
FT /ligand_id="ChEBI:CHEBI:37565"
FT /evidence="ECO:0000250|UniProtKB:Q8IZ41"
FT BINDING 585..586
FT /ligand="GTP"
FT /ligand_id="ChEBI:CHEBI:37565"
FT /evidence="ECO:0000250|UniProtKB:Q8IZ41"
SQ SEQUENCE 634 AA; 72272 MW; 3E4176A62C8A8048 CRC64;
MSKPEVENLF SLCDSESKGY LTMEDLRKVC PQLDDNDLRF IFTELDQDGS GKIEKLEFLR
GFQDTVQHGE SHGLNGMQRR ASVAVEDPPM FLRDEQMFDS ESDSTSSRPA IRVFDEEHYH
SESDTNINID FSVPCQEEVL VLYEQLQSSG VPALLRKFER VVGSFHKELS EKKHENERLQ
RIYASEREMY NRRMEEMESE VDQQLELTEM KARQEERDRL TKEKEEMRQR MSDEMSEMRN
NIERLQKMEK ALERENERLN HQKELSDKLK VVNEENNDLR QNLAENHLEL AMIKSELAQV
RCEFDQKQDE LSARRDQASH ATEESESVRK QLQLLFDANR KLHETNESLR DALDSRASVL
RQFNLRTPSP GLLSSNRNSV ENFQTSTNVF RSVPLHAIST EEQVPETSLI LDDAHSLQGL
DTPGDLMGLN DANGPAERTF RIVMCGDAAV GKSSFVMRVI RRQFTNQLPS TLGVDFHVKT
VNVDGRNVAL QLWDTAGQER FRSLCKSYFR RADGAILVYD VCAEQSFLRV RDWIETIKES
TERSIPIILV GNKVDMRIST PGSVAKTDGA SMAAAMGVLF METSALDGSN IDNAMLALTR
ELMAVEDVEI RSTGVVLNPA VAKKGGCFSK CRGS
