RASEF_MOUSE
ID RASEF_MOUSE Reviewed; 627 AA.
AC Q5RI75; B2RQ59; Q5RI76;
DT 11-SEP-2007, integrated into UniProtKB/Swiss-Prot.
DT 21-DEC-2004, sequence version 1.
DT 03-AUG-2022, entry version 141.
DE RecName: Full=Ras and EF-hand domain-containing protein homolog;
GN Name=Rasef;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC STRAIN=C57BL/6J; TISSUE=Testis;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C57BL/6J;
RX PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA Eichler E.E., Ponting C.P.;
RT "Lineage-specific biology revealed by a finished genome assembly of the
RT mouse.";
RL PLoS Biol. 7:E1000112-E1000112(2009).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC TISSUE=Brain;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [4]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-266 AND SER-272, AND
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Pancreas;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
CC -!- FUNCTION: Binds predominantly GDP, and also GTP (By similarity). Acts
CC as a dynein adapter protein that activates dynein-mediated transport
CC and dynein-dynactin motility on microtubules (By similarity).
CC {ECO:0000250|UniProtKB:Q8IZ41}.
CC -!- SUBUNIT: Homodimer (By similarity). Interacts with the dynein-dynactin
CC complex (By similarity). {ECO:0000250|UniProtKB:Q8IZ41}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm, perinuclear region
CC {ECO:0000250|UniProtKB:Q8IZ41}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q5RI75-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q5RI75-2; Sequence=VSP_027768;
CC -!- SIMILARITY: Belongs to the small GTPase superfamily. Rab family.
CC {ECO:0000305}.
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DR EMBL; AK132691; BAE21304.1; -; mRNA.
DR EMBL; BX294159; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; BC125539; AAI25540.1; -; mRNA.
DR EMBL; BC137774; AAI37775.1; -; mRNA.
DR CCDS; CCDS18281.1; -. [Q5RI75-1]
DR RefSeq; NP_001017427.1; NM_001017427.1. [Q5RI75-1]
DR RefSeq; XP_017175690.1; XM_017320201.1. [Q5RI75-1]
DR AlphaFoldDB; Q5RI75; -.
DR SMR; Q5RI75; -.
DR STRING; 10090.ENSMUSP00000062771; -.
DR iPTMnet; Q5RI75; -.
DR PhosphoSitePlus; Q5RI75; -.
DR EPD; Q5RI75; -.
DR jPOST; Q5RI75; -.
DR PaxDb; Q5RI75; -.
DR PeptideAtlas; Q5RI75; -.
DR PRIDE; Q5RI75; -.
DR ProteomicsDB; 300353; -. [Q5RI75-1]
DR ProteomicsDB; 300354; -. [Q5RI75-2]
DR Antibodypedia; 13004; 103 antibodies from 25 providers.
DR DNASU; 242505; -.
DR Ensembl; ENSMUST00000058292; ENSMUSP00000062771; ENSMUSG00000043003. [Q5RI75-1]
DR Ensembl; ENSMUST00000102837; ENSMUSP00000099901; ENSMUSG00000043003. [Q5RI75-2]
DR GeneID; 242505; -.
DR KEGG; mmu:242505; -.
DR UCSC; uc008tiw.1; mouse. [Q5RI75-1]
DR CTD; 158158; -.
DR MGI; MGI:2448565; Rasef.
DR VEuPathDB; HostDB:ENSMUSG00000043003; -.
DR eggNOG; KOG0078; Eukaryota.
DR GeneTree; ENSGT00940000159488; -.
DR HOGENOM; CLU_023178_1_0_1; -.
DR InParanoid; Q5RI75; -.
DR PhylomeDB; Q5RI75; -.
DR TreeFam; TF313106; -.
DR BioGRID-ORCS; 242505; 2 hits in 71 CRISPR screens.
DR ChiTaRS; Rasef; mouse.
DR PRO; PR:Q5RI75; -.
DR Proteomes; UP000000589; Chromosome 4.
DR RNAct; Q5RI75; protein.
DR Bgee; ENSMUSG00000043003; Expressed in epithelium of stomach and 63 other tissues.
DR ExpressionAtlas; Q5RI75; baseline and differential.
DR Genevisible; Q5RI75; MM.
DR GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR GO; GO:0005829; C:cytosol; ISO:MGI.
DR GO; GO:0048471; C:perinuclear region of cytoplasm; ISO:MGI.
DR GO; GO:0019003; F:GDP binding; ISO:MGI.
DR GO; GO:0005525; F:GTP binding; ISO:MGI.
DR GO; GO:0003924; F:GTPase activity; IEA:InterPro.
DR GO; GO:0042802; F:identical protein binding; ISO:MGI.
DR Gene3D; 3.40.50.300; -; 1.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR005225; Small_GTP-bd_dom.
DR InterPro; IPR001806; Small_GTPase.
DR Pfam; PF00071; Ras; 1.
DR SMART; SM00174; RHO; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
DR TIGRFAMs; TIGR00231; small_GTP; 1.
DR PROSITE; PS51419; RAB; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Coiled coil; Cytoplasm; GTP-binding;
KW Nucleotide-binding; Phosphoprotein; Reference proteome.
FT CHAIN 1..627
FT /note="Ras and EF-hand domain-containing protein homolog"
FT /id="PRO_0000299578"
FT COILED 55..245
FT /evidence="ECO:0000255"
FT BINDING 438..443
FT /ligand="GTP"
FT /ligand_id="ChEBI:CHEBI:37565"
FT /evidence="ECO:0000250|UniProtKB:Q8IZ41"
FT BINDING 541..544
FT /ligand="GTP"
FT /ligand_id="ChEBI:CHEBI:37565"
FT /evidence="ECO:0000250|UniProtKB:Q8IZ41"
FT BINDING 578..579
FT /ligand="GTP"
FT /ligand_id="ChEBI:CHEBI:37565"
FT /evidence="ECO:0000250|UniProtKB:Q8IZ41"
FT MOD_RES 266
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:21183079"
FT MOD_RES 272
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:21183079"
FT VAR_SEQ 1..72
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:16141072"
FT /id="VSP_027768"
SQ SEQUENCE 627 AA; 70752 MW; 3D9530B81BDF339D CRC64;
MAQEVSSSVA FIHRLLYTAL RGLLGDRLKR GLKKEQVSTL YQNITLVEPR LLQPYERVIR
NFLREIKLQS TEMENLAIAV KRAQDKAAIQ LSELEEEMDQ RIQAVENESR KDEKRKAEEA
LTDLRRQYET EVGDLQVTIK RLKKLEEQSR QISQKQDVTA LKKQIHDLTM ENQKLKKELL
EAQTNVAFLQ SELDALKSDY ADQSLNSERD LEIIREYTED RSSLERQIEI LQTANRKLHD
SNDGLRSALE NTYSKLNRSL RINNISPGNT ISRSSPKFNH HSSQPLAYDR SFHSSYADED
CDSLALCDPL QKMNYEVDSL PESCFDSGLS TLRDNECDSE VDYKHQGEFQ TLHRTEESLG
GDASDTDVPD IRDEEAFDSE SVASVLHWQP QGSAGEGSTL SSSRKPISAL SLQTDMVDNT
SKVTSQKAYK IVLAGDAAVG KSSFLMRLCK NEFQGNTSAT LGVDFQMKTL IVDGEQTVLQ
LWDTAGQERF RSIAKSYFRK ADGVLLLYDV TCEKSFLNVR EWVDMVEDGT HRTIPIMLVG
NKADLRDVDN AENQKCISAY LGEKLAMTYG ALFCETSAKD GSNVVEAVLH LAREVKKRTE
DDDSRSITSL AGSTSKKSLQ MKNCCNG
