RASE_HUMAN
ID RASE_HUMAN Reviewed; 233 AA.
AC Q7Z444;
DT 25-JUL-2006, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2003, sequence version 1.
DT 03-AUG-2022, entry version 151.
DE RecName: Full=GTPase ERas;
DE Short=E-Ras;
DE EC=3.6.5.2 {ECO:0000250|UniProtKB:P01116};
DE AltName: Full=Embryonic stem cell-expressed Ras;
DE Flags: Precursor;
GN Name=ERAS; Synonyms=HRAS2, HRASP;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RX PubMed=12774123; DOI=10.1038/nature01646;
RA Takahashi K., Mitsui K., Yamanaka S.;
RT "Role of Eras in promoting tumor-like properties in mouse embryonic stem
RT cells.";
RL Nature 423:541-545(2003).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Brain;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
CC -!- FUNCTION: Ras proteins bind GDP/GTP and possess intrinsic GTPase
CC activity. Plays an important role in the tumor-like growth properties
CC of embryonic stem cells (By similarity). {ECO:0000250}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=GTP + H2O = GDP + H(+) + phosphate; Xref=Rhea:RHEA:19669,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:37565,
CC ChEBI:CHEBI:43474, ChEBI:CHEBI:58189; EC=3.6.5.2;
CC Evidence={ECO:0000250|UniProtKB:P01116};
CC -!- ACTIVITY REGULATION: Alternates between an inactive form bound to GDP
CC and an active form bound to GTP. Activated by a guanine nucleotide-
CC exchange factor (GEF) and inactivated by a GTPase-activating protein
CC (GAP).
CC -!- SUBUNIT: Interacts with PIK3CD. {ECO:0000250}.
CC -!- INTERACTION:
CC Q7Z444; A0A024R8L2: hCG_1987119; NbExp=3; IntAct=EBI-13351543, EBI-14103818;
CC Q7Z444; O43711: TLX3; NbExp=3; IntAct=EBI-13351543, EBI-3939165;
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000250}; Lipid-anchor
CC {ECO:0000250}; Cytoplasmic side {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the small GTPase superfamily. Ras family.
CC {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AB093575; BAC76997.1; -; mRNA.
DR EMBL; BC101642; AAI01643.1; -; mRNA.
DR EMBL; BC101644; AAI01645.1; -; mRNA.
DR CCDS; CCDS35246.1; -.
DR RefSeq; NP_853510.1; NM_181532.3.
DR AlphaFoldDB; Q7Z444; -.
DR SMR; Q7Z444; -.
DR BioGRID; 109502; 4.
DR IntAct; Q7Z444; 2.
DR STRING; 9606.ENSP00000339136; -.
DR PhosphoSitePlus; Q7Z444; -.
DR BioMuta; ERAS; -.
DR DMDM; 74723320; -.
DR MassIVE; Q7Z444; -.
DR PaxDb; Q7Z444; -.
DR PeptideAtlas; Q7Z444; -.
DR PRIDE; Q7Z444; -.
DR Antibodypedia; 25880; 433 antibodies from 31 providers.
DR DNASU; 3266; -.
DR Ensembl; ENST00000338270.1; ENSP00000339136.1; ENSG00000187682.2.
DR Ensembl; ENST00000636362.1; ENSP00000490793.1; ENSG00000187682.2.
DR GeneID; 3266; -.
DR KEGG; hsa:3266; -.
DR MANE-Select; ENST00000636362.1; ENSP00000490793.1; NM_181532.3; NP_853510.1.
DR UCSC; uc064zbl.1; human.
DR CTD; 3266; -.
DR DisGeNET; 3266; -.
DR GeneCards; ERAS; -.
DR HGNC; HGNC:5174; ERAS.
DR HPA; ENSG00000187682; Tissue enhanced (brain).
DR MIM; 300437; gene.
DR neXtProt; NX_Q7Z444; -.
DR OpenTargets; ENSG00000187682; -.
DR PharmGKB; PA29448; -.
DR VEuPathDB; HostDB:ENSG00000187682; -.
DR eggNOG; KOG0395; Eukaryota.
DR GeneTree; ENSGT00940000162901; -.
DR HOGENOM; CLU_041217_9_8_1; -.
DR InParanoid; Q7Z444; -.
DR OMA; KAMCRCG; -.
DR OrthoDB; 1259506at2759; -.
DR PhylomeDB; Q7Z444; -.
DR TreeFam; TF312796; -.
DR PathwayCommons; Q7Z444; -.
DR SignaLink; Q7Z444; -.
DR BioGRID-ORCS; 3266; 9 hits in 698 CRISPR screens.
DR ChiTaRS; ERAS; human.
DR GenomeRNAi; 3266; -.
DR Pharos; Q7Z444; Tbio.
DR PRO; PR:Q7Z444; -.
DR Proteomes; UP000005640; Chromosome X.
DR RNAct; Q7Z444; protein.
DR Bgee; ENSG00000187682; Expressed in hypothalamus and 72 other tissues.
DR GO; GO:0005886; C:plasma membrane; IBA:GO_Central.
DR GO; GO:0003925; F:G protein activity; IEA:UniProtKB-EC.
DR GO; GO:0019003; F:GDP binding; IBA:GO_Central.
DR GO; GO:0005525; F:GTP binding; IBA:GO_Central.
DR GO; GO:0003924; F:GTPase activity; IBA:GO_Central.
DR GO; GO:0007265; P:Ras protein signal transduction; IBA:GO_Central.
DR Gene3D; 3.40.50.300; -; 1.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR005225; Small_GTP-bd_dom.
DR InterPro; IPR001806; Small_GTPase.
DR InterPro; IPR020849; Small_GTPase_Ras-type.
DR PANTHER; PTHR24070; PTHR24070; 1.
DR Pfam; PF00071; Ras; 1.
DR SMART; SM00174; RHO; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
DR TIGRFAMs; TIGR00231; small_GTP; 1.
DR PROSITE; PS51421; RAS; 1.
PE 1: Evidence at protein level;
KW Cell membrane; GTP-binding; Hydrolase; Lipoprotein; Membrane; Methylation;
KW Nucleotide-binding; Palmitate; Prenylation; Reference proteome.
FT CHAIN 1..230
FT /note="GTPase ERas"
FT /id="PRO_0000247538"
FT PROPEP 231..233
FT /note="Removed in mature form"
FT /evidence="ECO:0000250"
FT /id="PRO_0000247539"
FT MOTIF 70..78
FT /note="Effector region"
FT BINDING 48..55
FT /ligand="GTP"
FT /ligand_id="ChEBI:CHEBI:37565"
FT /evidence="ECO:0000250"
FT BINDING 95..99
FT /ligand="GTP"
FT /ligand_id="ChEBI:CHEBI:37565"
FT /evidence="ECO:0000250"
FT BINDING 151..154
FT /ligand="GTP"
FT /ligand_id="ChEBI:CHEBI:37565"
FT /evidence="ECO:0000250"
FT MOD_RES 230
FT /note="Cysteine methyl ester"
FT /evidence="ECO:0000250"
FT LIPID 226
FT /note="S-palmitoyl cysteine"
FT /evidence="ECO:0000250"
FT LIPID 228
FT /note="S-palmitoyl cysteine"
FT /evidence="ECO:0000250"
FT LIPID 230
FT /note="S-farnesyl cysteine"
FT /evidence="ECO:0000250"
SQ SEQUENCE 233 AA; 25287 MW; D5451F95978E7823 CRC64;
MELPTKPGTF DLGLATWSPS FQGETHRAQA RRRDVGRQLP EYKAVVVGAS GVGKSALTIQ
LNHQCFVEDH DPTIQDSYWK ELTLDSGDCI LNVLDTAGQA IHRALRDQCL AVCDGVLGVF
ALDDPSSLIQ LQQIWATWGP HPAQPLVLVG NKCDLVTTAG DAHAAAAALA HSWGAHFVET
SAKTRQGVEE AFSLLVHEIQ RVQEAMAKEP MARSCREKTR HQKATCHCGC SVA
