RASF3_HUMAN
ID RASF3_HUMAN Reviewed; 238 AA.
AC Q86WH2; Q86WH1;
DT 27-JUN-2006, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2003, sequence version 1.
DT 03-AUG-2022, entry version 141.
DE RecName: Full=Ras association domain-containing protein 3;
GN Name=RASSF3;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2).
RA Burbee D.G., White M.A., Miller D.S., Minna J.D., Muller C.Y.;
RT "RASSF5 is transcriptionally inactivated by an epigenetic mechanism in
RT ovarian cancers.";
RL Submitted (JAN-2003) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [3]
RP TISSUE SPECIFICITY, AND VARIANT HIS-232.
RX PubMed=11965544; DOI=10.1038/sj.onc.1205365;
RA Tommasi S., Dammann R., Jin S.-G., Zhang X.-F., Avruch J., Pfeifer G.P.;
RT "RASSF3 and NORE1: identification and cloning of two human homologues of
RT the putative tumor suppressor gene RASSF1.";
RL Oncogene 21:2713-2720(2002).
RN [4]
RP SUBCELLULAR LOCATION.
RX PubMed=15569673; DOI=10.1074/jbc.m409701200;
RA Fujita H., Fukuhara S., Sakurai A., Yamagishi A., Kamioka Y., Nakaoka Y.,
RA Masuda M., Mochizuki N.;
RT "Local activation of Rap1 contributes to directional vascular endothelial
RT cell migration accompanied by extension of microtubules on which RAPL, a
RT Rap1-associating molecule, localizes.";
RL J. Biol. Chem. 280:5022-5031(2005).
RN [5]
RP ACETYLATION [LARGE SCALE ANALYSIS] AT SER-2, CLEAVAGE OF INITIATOR
RP METHIONINE [LARGE SCALE ANALYSIS], AND IDENTIFICATION BY MASS SPECTROMETRY
RP [LARGE SCALE ANALYSIS].
RX PubMed=19369195; DOI=10.1074/mcp.m800588-mcp200;
RA Oppermann F.S., Gnad F., Olsen J.V., Hornberger R., Greff Z., Keri G.,
RA Mann M., Daub H.;
RT "Large-scale proteomics analysis of the human kinome.";
RL Mol. Cell. Proteomics 8:1751-1764(2009).
CC -!- INTERACTION:
CC Q86WH2; Q9HC52: CBX8; NbExp=3; IntAct=EBI-2845202, EBI-712912;
CC Q86WH2; Q9UER7: DAXX; NbExp=3; IntAct=EBI-2845202, EBI-77321;
CC Q86WH2; Q9BQ89: FAM110A; NbExp=3; IntAct=EBI-2845202, EBI-1752811;
CC Q86WH2; Q8IWB1: ITPRIP; NbExp=3; IntAct=EBI-2845202, EBI-2556412;
CC Q86WH2; O60341: KDM1A; NbExp=3; IntAct=EBI-2845202, EBI-710124;
CC Q86WH2; Q16719-2: KYNU; NbExp=3; IntAct=EBI-2845202, EBI-12351611;
CC Q86WH2; Q99732: LITAF; NbExp=4; IntAct=EBI-2845202, EBI-725647;
CC Q86WH2; Q9BYG5: PARD6B; NbExp=3; IntAct=EBI-2845202, EBI-295391;
CC Q86WH2; Q86WH2: RASSF3; NbExp=2; IntAct=EBI-2845202, EBI-2845202;
CC Q86WH2; Q9NQG5: RPRD1B; NbExp=3; IntAct=EBI-2845202, EBI-747925;
CC Q86WH2; Q13188: STK3; NbExp=7; IntAct=EBI-2845202, EBI-992580;
CC Q86WH2; Q13043: STK4; NbExp=6; IntAct=EBI-2845202, EBI-367376;
CC Q86WH2; Q8NEK8: TENT5D; NbExp=3; IntAct=EBI-2845202, EBI-744726;
CC Q86WH2; P02786: TFRC; NbExp=3; IntAct=EBI-2845202, EBI-355727;
CC Q86WH2; Q8IWR1: TRIM59; NbExp=3; IntAct=EBI-2845202, EBI-10262539;
CC Q86WH2; Q86VK4-3: ZNF410; NbExp=3; IntAct=EBI-2845202, EBI-11741890;
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:15569673}.
CC Cytoplasm, cytoskeleton {ECO:0000269|PubMed:15569673}. Note=Localized
CC to microtubules in vascular endothelial cells.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1; Synonyms=A;
CC IsoId=Q86WH2-1; Sequence=Displayed;
CC Name=2; Synonyms=B;
CC IsoId=Q86WH2-2; Sequence=VSP_019353, VSP_019354;
CC -!- TISSUE SPECIFICITY: Widely expressed. {ECO:0000269|PubMed:11965544}.
CC -!- CAUTION: Was termed (Ref.1) RASSF5. {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AY217662; AAO61687.1; -; mRNA.
DR EMBL; AY217663; AAO61688.1; -; mRNA.
DR EMBL; BC100951; AAI00952.1; -; mRNA.
DR EMBL; BC100949; AAI00950.1; -; mRNA.
DR CCDS; CCDS8969.1; -. [Q86WH2-1]
DR RefSeq; NP_835463.1; NM_178169.3. [Q86WH2-1]
DR AlphaFoldDB; Q86WH2; -.
DR SMR; Q86WH2; -.
DR BioGRID; 129534; 34.
DR IntAct; Q86WH2; 38.
DR STRING; 9606.ENSP00000443021; -.
DR iPTMnet; Q86WH2; -.
DR PhosphoSitePlus; Q86WH2; -.
DR BioMuta; RASSF3; -.
DR DMDM; 74714093; -.
DR EPD; Q86WH2; -.
DR jPOST; Q86WH2; -.
DR MassIVE; Q86WH2; -.
DR MaxQB; Q86WH2; -.
DR PaxDb; Q86WH2; -.
DR PeptideAtlas; Q86WH2; -.
DR PRIDE; Q86WH2; -.
DR ProteomicsDB; 70160; -. [Q86WH2-1]
DR ProteomicsDB; 70161; -. [Q86WH2-2]
DR Antibodypedia; 29184; 197 antibodies from 32 providers.
DR DNASU; 283349; -.
DR Ensembl; ENST00000283172.8; ENSP00000283172.4; ENSG00000153179.13. [Q86WH2-2]
DR Ensembl; ENST00000336061.2; ENSP00000336616.2; ENSG00000153179.13. [Q86WH2-1]
DR Ensembl; ENST00000542104.6; ENSP00000443021.1; ENSG00000153179.13. [Q86WH2-1]
DR GeneID; 283349; -.
DR KEGG; hsa:283349; -.
DR MANE-Select; ENST00000542104.6; ENSP00000443021.1; NM_178169.4; NP_835463.1.
DR UCSC; uc001ssd.3; human. [Q86WH2-1]
DR CTD; 283349; -.
DR DisGeNET; 283349; -.
DR GeneCards; RASSF3; -.
DR HGNC; HGNC:14271; RASSF3.
DR HPA; ENSG00000153179; Low tissue specificity.
DR MIM; 607019; gene.
DR neXtProt; NX_Q86WH2; -.
DR OpenTargets; ENSG00000153179; -.
DR PharmGKB; PA34247; -.
DR VEuPathDB; HostDB:ENSG00000153179; -.
DR eggNOG; KOG4239; Eukaryota.
DR GeneTree; ENSGT00940000157502; -.
DR HOGENOM; CLU_2440221_0_0_1; -.
DR InParanoid; Q86WH2; -.
DR OrthoDB; 1120975at2759; -.
DR PhylomeDB; Q86WH2; -.
DR TreeFam; TF319243; -.
DR PathwayCommons; Q86WH2; -.
DR SignaLink; Q86WH2; -.
DR BioGRID-ORCS; 283349; 13 hits in 1077 CRISPR screens.
DR ChiTaRS; RASSF3; human.
DR GenomeRNAi; 283349; -.
DR Pharos; Q86WH2; Tbio.
DR PRO; PR:Q86WH2; -.
DR Proteomes; UP000005640; Chromosome 12.
DR RNAct; Q86WH2; protein.
DR Bgee; ENSG00000153179; Expressed in saphenous vein and 194 other tissues.
DR ExpressionAtlas; Q86WH2; baseline and differential.
DR Genevisible; Q86WH2; HS.
DR GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR GO; GO:0005829; C:cytosol; IDA:HPA.
DR GO; GO:0005874; C:microtubule; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IDA:HPA.
DR GO; GO:0042802; F:identical protein binding; IPI:IntAct.
DR GO; GO:0042981; P:regulation of apoptotic process; IEA:InterPro.
DR GO; GO:0007165; P:signal transduction; IBA:GO_Central.
DR InterPro; IPR000159; RA_dom.
DR InterPro; IPR033614; RASSF1-6.
DR InterPro; IPR033619; RASSF3.
DR InterPro; IPR011524; SARAH_dom.
DR InterPro; IPR029071; Ubiquitin-like_domsf.
DR PANTHER; PTHR22738; PTHR22738; 1.
DR PANTHER; PTHR22738:SF8; PTHR22738:SF8; 1.
DR Pfam; PF16517; Nore1-SARAH; 1.
DR Pfam; PF00788; RA; 1.
DR SMART; SM00314; RA; 1.
DR SUPFAM; SSF54236; SSF54236; 1.
DR PROSITE; PS50200; RA; 1.
DR PROSITE; PS50951; SARAH; 1.
PE 1: Evidence at protein level;
KW Acetylation; Alternative splicing; Cytoplasm; Cytoskeleton; Microtubule;
KW Reference proteome.
FT INIT_MET 1
FT /note="Removed"
FT /evidence="ECO:0007744|PubMed:19369195"
FT CHAIN 2..238
FT /note="Ras association domain-containing protein 3"
FT /id="PRO_0000240396"
FT DOMAIN 79..186
FT /note="Ras-associating"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00166"
FT DOMAIN 187..234
FT /note="SARAH"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00310"
FT REGION 26..48
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 2
FT /note="N-acetylserine"
FT /evidence="ECO:0007744|PubMed:19369195"
FT VAR_SEQ 74..90
FT /note="NSNGIYTGFIKVQMELC -> STPASSQTGNIHSTCVW (in isoform
FT 2)"
FT /evidence="ECO:0000303|Ref.1"
FT /id="VSP_019353"
FT VAR_SEQ 91..238
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|Ref.1"
FT /id="VSP_019354"
FT VARIANT 232
FT /note="R -> H (in dbSNP:rs74098110)"
FT /evidence="ECO:0000269|PubMed:11965544"
FT /id="VAR_026724"
SQ SEQUENCE 238 AA; 27562 MW; 2C8AD10649259811 CRC64;
MSSGYSSLEE DAEDFFFTAR TSFFRRAPQG KPRSGQQDVE KEKETHSYLS KEEIKEKVHK
YNLAVTDKLK MTLNSNGIYT GFIKVQMELC KPPQTSPNSG KLSPSSNGCM NTLHISSTNT
VGEVIEALLK KFLVTESPAK FALYKRCHRE DQVYACKLSD REHPLYLRLV AGPRTDTLSF
VLREHEIGEW EAFSLPELQN FLRILDKEED EQLQNLKRRY TAYRQKLEEA LREVWKPD
