RASF4_MOUSE
ID RASF4_MOUSE Reviewed; 322 AA.
AC Q8CB96; Q3TBK1; Q3TPH9; Q3TUT0; Q6P9L7; Q8CIM6;
DT 27-JUN-2006, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2003, sequence version 1.
DT 03-AUG-2022, entry version 117.
DE RecName: Full=Ras association domain-containing protein 4;
GN Name=Rassf4;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
RC STRAIN=C57BL/6J, and NOD; TISSUE=Bone, Cerebellum, and Spinal ganglion;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 3).
RC STRAIN=C57BL/6J, and FVB/N; TISSUE=Brain, and Mammary tumor;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [3]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-142, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Spleen;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
CC -!- FUNCTION: Potential tumor suppressor. May act as a KRAS effector
CC protein. May promote apoptosis and cell cycle arrest.
CC -!- SUBUNIT: Interacts directly with activated KRAS in a GTP-dependent
CC manner. {ECO:0000250}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=3;
CC Name=1;
CC IsoId=Q8CB96-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q8CB96-2; Sequence=VSP_019362;
CC Name=3;
CC IsoId=Q8CB96-3; Sequence=VSP_019360, VSP_019361;
CC -!- SEQUENCE CAUTION:
CC Sequence=BAE42308.1; Type=Frameshift; Evidence={ECO:0000305};
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AK036528; BAC29463.1; -; mRNA.
DR EMBL; AK160584; BAE35891.1; -; mRNA.
DR EMBL; AK164357; BAE37757.1; -; mRNA.
DR EMBL; AK170955; BAE42138.1; -; mRNA.
DR EMBL; AK171198; BAE42308.1; ALT_FRAME; mRNA.
DR EMBL; AK139284; BAE23943.1; -; mRNA.
DR EMBL; BC023245; AAH23245.1; -; mRNA.
DR EMBL; BC060709; AAH60709.1; -; mRNA.
DR CCDS; CCDS20460.1; -. [Q8CB96-1]
DR RefSeq; NP_835146.3; NM_178045.4. [Q8CB96-1]
DR AlphaFoldDB; Q8CB96; -.
DR STRING; 10090.ENSMUSP00000048267; -.
DR iPTMnet; Q8CB96; -.
DR PhosphoSitePlus; Q8CB96; -.
DR MaxQB; Q8CB96; -.
DR PaxDb; Q8CB96; -.
DR PeptideAtlas; Q8CB96; -.
DR PRIDE; Q8CB96; -.
DR ProteomicsDB; 300236; -. [Q8CB96-1]
DR ProteomicsDB; 300237; -. [Q8CB96-2]
DR ProteomicsDB; 300238; -. [Q8CB96-3]
DR Antibodypedia; 26967; 184 antibodies from 28 providers.
DR DNASU; 213391; -.
DR Ensembl; ENSMUST00000035842; ENSMUSP00000048267; ENSMUSG00000042129. [Q8CB96-1]
DR Ensembl; ENSMUST00000203029; ENSMUSP00000144786; ENSMUSG00000042129. [Q8CB96-2]
DR GeneID; 213391; -.
DR KEGG; mmu:213391; -.
DR UCSC; uc009dkm.2; mouse. [Q8CB96-1]
DR UCSC; uc009dko.3; mouse. [Q8CB96-3]
DR CTD; 83937; -.
DR MGI; MGI:2386853; Rassf4.
DR VEuPathDB; HostDB:ENSMUSG00000042129; -.
DR eggNOG; KOG4239; Eukaryota.
DR GeneTree; ENSGT00940000160176; -.
DR HOGENOM; CLU_018893_1_0_1; -.
DR InParanoid; Q8CB96; -.
DR OMA; HAPGEAQ; -.
DR OrthoDB; 1043350at2759; -.
DR PhylomeDB; Q8CB96; -.
DR TreeFam; TF319243; -.
DR BioGRID-ORCS; 213391; 2 hits in 73 CRISPR screens.
DR ChiTaRS; Rassf4; mouse.
DR PRO; PR:Q8CB96; -.
DR Proteomes; UP000000589; Chromosome 6.
DR RNAct; Q8CB96; protein.
DR Bgee; ENSMUSG00000042129; Expressed in vestibular membrane of cochlear duct and 177 other tissues.
DR ExpressionAtlas; Q8CB96; baseline and differential.
DR Genevisible; Q8CB96; MM.
DR GO; GO:0007049; P:cell cycle; IEA:UniProtKB-KW.
DR GO; GO:0007165; P:signal transduction; IBA:GO_Central.
DR InterPro; IPR000159; RA_dom.
DR InterPro; IPR033614; RASSF1-6.
DR InterPro; IPR033622; RASSF4.
DR InterPro; IPR011524; SARAH_dom.
DR InterPro; IPR029071; Ubiquitin-like_domsf.
DR PANTHER; PTHR22738; PTHR22738; 1.
DR PANTHER; PTHR22738:SF4; PTHR22738:SF4; 1.
DR Pfam; PF16517; Nore1-SARAH; 1.
DR Pfam; PF00788; RA; 1.
DR SMART; SM00314; RA; 1.
DR SUPFAM; SSF54236; SSF54236; 1.
DR PROSITE; PS50200; RA; 1.
DR PROSITE; PS50951; SARAH; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Cell cycle; Phosphoprotein; Reference proteome;
KW Tumor suppressor.
FT CHAIN 1..322
FT /note="Ras association domain-containing protein 4"
FT /id="PRO_0000240399"
FT DOMAIN 175..264
FT /note="Ras-associating"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00166"
FT DOMAIN 271..318
FT /note="SARAH"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00310"
FT REGION 96..161
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 142
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:21183079"
FT VAR_SEQ 179..276
FT /note="TSVFTPAYGSVTNVRVNSTMTTQQVLTLLLNKFRVEDGPSEFALYTVHESGE
FT QTKLKDCEYPLISRILHGPCEKIVKIFLMEADLSEEVPHDVAQYIK -> VKSPSALSW
FT ALKLRVQELFPSTEERAPNIQHSSHSSIR (in isoform 3)"
FT /evidence="ECO:0000303|PubMed:15489334"
FT /id="VSP_019360"
FT VAR_SEQ 277..322
FT /note="Missing (in isoform 3)"
FT /evidence="ECO:0000303|PubMed:15489334"
FT /id="VSP_019361"
FT VAR_SEQ 304..322
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:16141072"
FT /id="VSP_019362"
FT CONFLICT 153
FT /note="K -> E (in Ref. 1; BAE42308)"
FT /evidence="ECO:0000305"
FT CONFLICT 291
FT /note="E -> G (in Ref. 1; BAE37757)"
FT /evidence="ECO:0000305"
FT CONFLICT 295
FT /note="R -> I (in Ref. 2; AAH60709)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 322 AA; 36934 MW; C8CEE2610DDEFCC5 CRC64;
MKEACSSSSH VPVSDSKYIL KSELLSLLKT YNCYHEGRSF QLRHREEEGT LIIEGLLNIA
WGLRRPIRLQ MQDDRERVHL PSATWVPERL SYLQKEASPQ DSKVPTEEPG TQPANKAEVS
GDSSGALEGE EEEVPQLMRT KSDASCIIQR RSKSRAPSEA QKIRRHRFSI NGHFYNHKTS
VFTPAYGSVT NVRVNSTMTT QQVLTLLLNK FRVEDGPSEF ALYTVHESGE QTKLKDCEYP
LISRILHGPC EKIVKIFLME ADLSEEVPHD VAQYIKFEMP VLDSFVEKLK EEEEREIIKL
TMKFQALRLT MLQRLEQLVE AK
