RASF6_MOUSE
ID RASF6_MOUSE Reviewed; 353 AA.
AC Q80UQ2; Q9DAW2;
DT 27-JUN-2006, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2003, sequence version 1.
DT 03-AUG-2022, entry version 114.
DE RecName: Full=Ras association domain-containing protein 6;
GN Name=Rassf6;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC STRAIN=C57BL/6J; TISSUE=Placenta;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC STRAIN=FVB/N; TISSUE=Mammary tumor;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [3]
RP FUNCTION, AND INTERACTION WITH KRAS AND MOAP1.
RX PubMed=17404571; DOI=10.1038/sj.onc.1210440;
RA Allen N.P., Donninger H., Vos M.D., Eckfeld K., Hesson L., Gordon L.,
RA Birrer M.J., Latif F., Clark G.J.;
RT "RASSF6 is a novel member of the RASSF family of tumor suppressors.";
RL Oncogene 26:6203-6211(2007).
RN [4]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-155, PHOSPHORYLATION [LARGE
RP SCALE ANALYSIS] AT SER-126 (ISOFORM 2), AND IDENTIFICATION BY MASS
RP SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Kidney;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
CC -!- FUNCTION: Involved in the induction of apoptosis. May act as a Ras
CC effector protein. May suppress the serum-induced basal levels of NF-
CC kappa-B. {ECO:0000269|PubMed:17404571}.
CC -!- SUBUNIT: Interacts with MOAP1. Interaction with activated KRAS is still
CC a matter of debate: interaction has been shown in the mouse
CC (PubMed:17404571), but not in human (PubMed:17367779). Lack of
CC interaction with MRAS, NRAS nor RRAS2 has also been reported
CC (PubMed:17367779). {ECO:0000269|PubMed:17404571}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q80UQ2-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q80UQ2-2; Sequence=VSP_019373, VSP_019374;
CC -!- SEQUENCE CAUTION:
CC Sequence=AAH39934.2; Type=Erroneous initiation; Note=Extended N-terminus.; Evidence={ECO:0000305};
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DR EMBL; AK005472; BAB24062.1; -; mRNA.
DR EMBL; AK132288; BAE21083.1; -; mRNA.
DR EMBL; BC039934; AAH39934.2; ALT_INIT; mRNA.
DR CCDS; CCDS80327.1; -. [Q80UQ2-2]
DR RefSeq; NP_001297585.1; NM_001310656.1. [Q80UQ2-2]
DR AlphaFoldDB; Q80UQ2; -.
DR SMR; Q80UQ2; -.
DR STRING; 10090.ENSMUSP00000031317; -.
DR iPTMnet; Q80UQ2; -.
DR PhosphoSitePlus; Q80UQ2; -.
DR MaxQB; Q80UQ2; -.
DR PaxDb; Q80UQ2; -.
DR PRIDE; Q80UQ2; -.
DR ProteomicsDB; 300306; -. [Q80UQ2-1]
DR ProteomicsDB; 300307; -. [Q80UQ2-2]
DR Antibodypedia; 24560; 208 antibodies from 33 providers.
DR DNASU; 73246; -.
DR Ensembl; ENSMUST00000202784; ENSMUSP00000144337; ENSMUSG00000029370. [Q80UQ2-2]
DR GeneID; 73246; -.
DR KEGG; mmu:73246; -.
DR UCSC; uc008ybe.1; mouse. [Q80UQ2-2]
DR CTD; 166824; -.
DR MGI; MGI:1920496; Rassf6.
DR VEuPathDB; HostDB:ENSMUSG00000029370; -.
DR eggNOG; KOG4239; Eukaryota.
DR GeneTree; ENSGT00940000159886; -.
DR InParanoid; Q80UQ2; -.
DR OrthoDB; 1043350at2759; -.
DR PhylomeDB; Q80UQ2; -.
DR BioGRID-ORCS; 73246; 2 hits in 72 CRISPR screens.
DR ChiTaRS; Rassf6; mouse.
DR PRO; PR:Q80UQ2; -.
DR Proteomes; UP000000589; Chromosome 5.
DR RNAct; Q80UQ2; protein.
DR Bgee; ENSMUSG00000029370; Expressed in placenta labyrinth and 105 other tissues.
DR ExpressionAtlas; Q80UQ2; baseline and differential.
DR GO; GO:0006915; P:apoptotic process; IEA:UniProtKB-KW.
DR GO; GO:0043065; P:positive regulation of apoptotic process; IDA:MGI.
DR GO; GO:0042981; P:regulation of apoptotic process; IDA:UniProtKB.
DR GO; GO:0007165; P:signal transduction; IBA:GO_Central.
DR InterPro; IPR000159; RA_dom.
DR InterPro; IPR033614; RASSF1-6.
DR InterPro; IPR033625; RASSF6.
DR InterPro; IPR011524; SARAH_dom.
DR InterPro; IPR029071; Ubiquitin-like_domsf.
DR PANTHER; PTHR22738; PTHR22738; 2.
DR PANTHER; PTHR22738:SF3; PTHR22738:SF3; 2.
DR Pfam; PF16517; Nore1-SARAH; 1.
DR Pfam; PF00788; RA; 1.
DR SMART; SM00314; RA; 1.
DR SUPFAM; SSF54236; SSF54236; 1.
DR PROSITE; PS50200; RA; 1.
DR PROSITE; PS50951; SARAH; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Apoptosis; Phosphoprotein; Reference proteome.
FT CHAIN 1..353
FT /note="Ras association domain-containing protein 6"
FT /id="PRO_0000240405"
FT DOMAIN 202..290
FT /note="Ras-associating"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00166"
FT DOMAIN 297..344
FT /note="SARAH"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00310"
FT MOD_RES 155
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:21183079"
FT VAR_SEQ 129..141
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:16141072"
FT /id="VSP_019373"
FT VAR_SEQ 148..159
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:16141072"
FT /id="VSP_019374"
FT MOD_RES Q80UQ2-2:126
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:21183079"
SQ SEQUENCE 353 AA; 41252 MW; 1787C9F5DA5A0494 CRC64;
MTAMDHQFPS WIVVNESTSI SREQLNYLLE TYNVFYENQK NLHILYGQTE DGQLIVEGML
DIFWGVKRPI QLKIQDEKQI SSFDLLNTPE TFSSKGRMTR WGEFDDLYRI SELDRTHVLA
SEARHSPEDY LSYHSTLTPY ADEEPESPLL YRTMSEAESP LLYRTMSEAA LVRKRMRAPE
MYRKDRMGVL SNHRASINGH VYDHETSIFT PTFGSETKVR ANSIMRTEEV IKQLLQKFKI
ENSPRDFALY IIFGTGEQRK LKKTDVPLLQ RLLQGPSKSN ARIFLMDKDA EEISRDVAPY
INFHFSFLES ILQRLDEEEK MEIERIMAKF NTERAFILKC LQSKQAAKTE TTV
