RASLC_HUMAN
ID RASLC_HUMAN Reviewed; 266 AA.
AC Q9NYN1; B2RC29; B4DJW2; B4DU82;
DT 20-MAY-2008, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2000, sequence version 1.
DT 03-AUG-2022, entry version 169.
DE RecName: Full=Ras-like protein family member 12;
DE EC=3.6.5.2 {ECO:0000250|UniProtKB:P01116};
DE AltName: Full=Ras-like protein Ris;
GN Name=RASL12; Synonyms=RIS;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
RC TISSUE=Iris;
RX PubMed=12107412;
RA Wistow G., Bernstein S.L., Ray S., Wyatt M.K., Behal A., Touchman J.W.,
RA Bouffard G., Smith D., Peterson K.;
RT "Expressed sequence tag analysis of adult human iris for the NEIBank
RT project: steroid-response factors and similarities with retinal pigment
RT epithelium.";
RL Mol. Vis. 8:185-195(2002).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1; 2 AND 3).
RC TISSUE=Brain, Prostate, and Thalamus;
RX PubMed=14702039; DOI=10.1038/ng1285;
RA Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H.,
RA Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M.,
RA Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K.,
RA Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T.,
RA Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M.,
RA Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S.,
RA Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H.,
RA Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K.,
RA Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N.,
RA Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y.,
RA Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K.,
RA Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T.,
RA Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T.,
RA Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y.,
RA Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H.,
RA Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y.,
RA Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H.,
RA Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O.,
RA Isogai T., Sugano S.;
RT "Complete sequencing and characterization of 21,243 full-length human
RT cDNAs.";
RL Nat. Genet. 36:40-45(2004).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=16572171; DOI=10.1038/nature04601;
RA Zody M.C., Garber M., Sharpe T., Young S.K., Rowen L., O'Neill K.,
RA Whittaker C.A., Kamal M., Chang J.L., Cuomo C.A., Dewar K.,
RA FitzGerald M.G., Kodira C.D., Madan A., Qin S., Yang X., Abbasi N.,
RA Abouelleil A., Arachchi H.M., Baradarani L., Birditt B., Bloom S.,
RA Bloom T., Borowsky M.L., Burke J., Butler J., Cook A., DeArellano K.,
RA DeCaprio D., Dorris L. III, Dors M., Eichler E.E., Engels R., Fahey J.,
RA Fleetwood P., Friedman C., Gearin G., Hall J.L., Hensley G., Johnson E.,
RA Jones C., Kamat A., Kaur A., Locke D.P., Madan A., Munson G., Jaffe D.B.,
RA Lui A., Macdonald P., Mauceli E., Naylor J.W., Nesbitt R., Nicol R.,
RA O'Leary S.B., Ratcliffe A., Rounsley S., She X., Sneddon K.M.B.,
RA Stewart S., Sougnez C., Stone S.M., Topham K., Vincent D., Wang S.,
RA Zimmer A.R., Birren B.W., Hood L., Lander E.S., Nusbaum C.;
RT "Analysis of the DNA sequence and duplication history of human chromosome
RT 15.";
RL Nature 440:671-675(2006).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M.,
RA Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J.,
RA Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S.,
RA Turner R., Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H.,
RA Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K.,
RA Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D.,
RA Hunkapiller M.W., Myers E.W., Venter J.C.;
RL Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC TISSUE=Ovary;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [6]
RP X-RAY CRYSTALLOGRAPHY (1.85 ANGSTROMS) OF 18-186 IN COMPLEX WITH GDP.
RG Structural genomics consortium (SGC);
RT "Crystal structure of human ras-like, family 12 protein in complex with
RT GDP.";
RL Submitted (FEB-2009) to the PDB data bank.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=GTP + H2O = GDP + H(+) + phosphate; Xref=Rhea:RHEA:19669,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:37565,
CC ChEBI:CHEBI:43474, ChEBI:CHEBI:58189; EC=3.6.5.2;
CC Evidence={ECO:0000250|UniProtKB:P01116};
CC -!- INTERACTION:
CC Q9NYN1; Q14192: FHL2; NbExp=4; IntAct=EBI-12917066, EBI-701903;
CC Q9NYN1; Q13643: FHL3; NbExp=3; IntAct=EBI-12917066, EBI-741101;
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=3;
CC Name=1;
CC IsoId=Q9NYN1-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q9NYN1-2; Sequence=VSP_055845;
CC Name=3;
CC IsoId=Q9NYN1-3; Sequence=VSP_055846;
CC -!- SIMILARITY: Belongs to the small GTPase superfamily. Ras family.
CC {ECO:0000305}.
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DR EMBL; AF233588; AAF60286.1; -; mRNA.
DR EMBL; AK296261; BAG58974.1; -; mRNA.
DR EMBL; AK300536; BAG62244.1; -; mRNA.
DR EMBL; AK314914; BAG37426.1; -; mRNA.
DR EMBL; AC013553; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; CH471082; EAW77709.1; -; Genomic_DNA.
DR EMBL; BC053734; AAH53734.1; -; mRNA.
DR CCDS; CCDS10200.1; -. [Q9NYN1-1]
DR CCDS; CCDS76769.1; -. [Q9NYN1-3]
DR RefSeq; NP_001294859.1; NM_001307930.1. [Q9NYN1-3]
DR RefSeq; NP_057647.1; NM_016563.3. [Q9NYN1-1]
DR PDB; 3C5C; X-ray; 1.85 A; A/B/C/D=18-186.
DR PDBsum; 3C5C; -.
DR AlphaFoldDB; Q9NYN1; -.
DR SMR; Q9NYN1; -.
DR BioGRID; 119437; 14.
DR IntAct; Q9NYN1; 3.
DR STRING; 9606.ENSP00000220062; -.
DR iPTMnet; Q9NYN1; -.
DR PhosphoSitePlus; Q9NYN1; -.
DR BioMuta; RASL12; -.
DR DMDM; 74734715; -.
DR MassIVE; Q9NYN1; -.
DR PaxDb; Q9NYN1; -.
DR PeptideAtlas; Q9NYN1; -.
DR PRIDE; Q9NYN1; -.
DR ProteomicsDB; 4411; -.
DR ProteomicsDB; 5160; -.
DR ProteomicsDB; 83255; -. [Q9NYN1-1]
DR Antibodypedia; 25905; 140 antibodies from 20 providers.
DR DNASU; 51285; -.
DR Ensembl; ENST00000220062.9; ENSP00000220062.4; ENSG00000103710.11. [Q9NYN1-1]
DR Ensembl; ENST00000421977.7; ENSP00000390028.3; ENSG00000103710.11. [Q9NYN1-3]
DR Ensembl; ENST00000434605.2; ENSP00000412787.2; ENSG00000103710.11. [Q9NYN1-2]
DR GeneID; 51285; -.
DR KEGG; hsa:51285; -.
DR MANE-Select; ENST00000220062.9; ENSP00000220062.4; NM_016563.4; NP_057647.1.
DR UCSC; uc002aoi.2; human. [Q9NYN1-1]
DR CTD; 51285; -.
DR DisGeNET; 51285; -.
DR GeneCards; RASL12; -.
DR HGNC; HGNC:30289; RASL12.
DR HPA; ENSG00000103710; Low tissue specificity.
DR neXtProt; NX_Q9NYN1; -.
DR OpenTargets; ENSG00000103710; -.
DR PharmGKB; PA134920029; -.
DR VEuPathDB; HostDB:ENSG00000103710; -.
DR eggNOG; KOG0395; Eukaryota.
DR GeneTree; ENSGT00940000160167; -.
DR HOGENOM; CLU_041217_9_4_1; -.
DR InParanoid; Q9NYN1; -.
DR OMA; QAEWNLP; -.
DR PhylomeDB; Q9NYN1; -.
DR TreeFam; TF318030; -.
DR PathwayCommons; Q9NYN1; -.
DR SignaLink; Q9NYN1; -.
DR BioGRID-ORCS; 51285; 11 hits in 1070 CRISPR screens.
DR ChiTaRS; RASL12; human.
DR EvolutionaryTrace; Q9NYN1; -.
DR GenomeRNAi; 51285; -.
DR Pharos; Q9NYN1; Tbio.
DR PRO; PR:Q9NYN1; -.
DR Proteomes; UP000005640; Chromosome 15.
DR RNAct; Q9NYN1; protein.
DR Bgee; ENSG00000103710; Expressed in popliteal artery and 170 other tissues.
DR ExpressionAtlas; Q9NYN1; baseline and differential.
DR Genevisible; Q9NYN1; HS.
DR GO; GO:0005886; C:plasma membrane; IBA:GO_Central.
DR GO; GO:0003925; F:G protein activity; IEA:UniProtKB-EC.
DR GO; GO:0019003; F:GDP binding; IBA:GO_Central.
DR GO; GO:0005525; F:GTP binding; IBA:GO_Central.
DR GO; GO:0003924; F:GTPase activity; IBA:GO_Central.
DR GO; GO:0007165; P:signal transduction; IEA:InterPro.
DR Gene3D; 3.40.50.300; -; 1.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR005225; Small_GTP-bd_dom.
DR InterPro; IPR001806; Small_GTPase.
DR InterPro; IPR020849; Small_GTPase_Ras-type.
DR PANTHER; PTHR24070; PTHR24070; 1.
DR Pfam; PF00071; Ras; 1.
DR SMART; SM00174; RHO; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
DR TIGRFAMs; TIGR00231; small_GTP; 1.
DR PROSITE; PS51421; RAS; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Alternative splicing; GTP-binding; Hydrolase;
KW Nucleotide-binding; Reference proteome.
FT CHAIN 1..266
FT /note="Ras-like protein family member 12"
FT /id="PRO_0000333868"
FT BINDING 27..34
FT /ligand="GTP"
FT /ligand_id="ChEBI:CHEBI:37565"
FT BINDING 74..78
FT /ligand="GTP"
FT /ligand_id="ChEBI:CHEBI:37565"
FT /evidence="ECO:0000250"
FT BINDING 134..137
FT /ligand="GTP"
FT /ligand_id="ChEBI:CHEBI:37565"
FT VAR_SEQ 1..34
FT /note="MSSVFGKPRAGSGPQSAPLEVNLAILGRRGAGKS -> MVIMGPELVGSWSD
FT FKNEAADPR (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:14702039"
FT /id="VSP_055845"
FT VAR_SEQ 35..53
FT /note="Missing (in isoform 3)"
FT /evidence="ECO:0000303|PubMed:14702039"
FT /id="VSP_055846"
FT STRAND 20..26
FT /evidence="ECO:0007829|PDB:3C5C"
FT HELIX 33..42
FT /evidence="ECO:0007829|PDB:3C5C"
FT STRAND 55..63
FT /evidence="ECO:0007829|PDB:3C5C"
FT STRAND 66..74
FT /evidence="ECO:0007829|PDB:3C5C"
FT HELIX 85..88
FT /evidence="ECO:0007829|PDB:3C5C"
FT STRAND 92..99
FT /evidence="ECO:0007829|PDB:3C5C"
FT HELIX 103..123
FT /evidence="ECO:0007829|PDB:3C5C"
FT STRAND 129..134
FT /evidence="ECO:0007829|PDB:3C5C"
FT HELIX 136..141
FT /evidence="ECO:0007829|PDB:3C5C"
FT HELIX 146..156
FT /evidence="ECO:0007829|PDB:3C5C"
FT STRAND 159..162
FT /evidence="ECO:0007829|PDB:3C5C"
FT STRAND 165..167
FT /evidence="ECO:0007829|PDB:3C5C"
FT HELIX 170..184
FT /evidence="ECO:0007829|PDB:3C5C"
SQ SEQUENCE 266 AA; 29662 MW; CEF9841F94BE9DBA CRC64;
MSSVFGKPRA GSGPQSAPLE VNLAILGRRG AGKSALTVKF LTKRFISEYD PNLEDTYSSE
ETVDHQPVHL RVMDTADLDT PRNCERYLNW AHAFLVVYSV DSRQSFDSSS SYLELLALHA
KETQRSIPAL LLGNKLDMAQ YRQVTKAEGV ALAGRFGCLF FEVSACLDFE HVQHVFHEAV
REARRELEKS PLTRPLFISE ERALPHQAPL TARHGLASCT FNTLSTINLK EMPTVAQAKL
VTVKSSRAQS KRKAPTLTLL KGFKIF
