ID   RAS_MELOI               Reviewed;         427 AA.
AC   G0LD36;
DT   29-MAY-2013, integrated into UniProtKB/Swiss-Prot.
DT   19-OCT-2011, sequence version 1.
DT   03-AUG-2022, entry version 27.
DE   RecName: Full=Rosmarinate synthase;
DE            Short=MoRAS;
DE            EC=2.3.1.140;
DE   AltName: Full=Hydroxycinnamoyl-CoA:hydroxyphenyllactate hydroxycinnamoyltransferase;
GN   Name=RAS;
OS   Melissa officinalis (Lemon balm).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   asterids; lamiids; Lamiales; Lamiaceae; Nepetoideae; Mentheae; Salviinae;
OC   Melissa.
OX   NCBI_TaxID=39338;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, CATALYTIC ACTIVITY, AND
RP   BIOPHYSICOCHEMICAL PROPERTIES.
RX   PubMed=21354582; DOI=10.1016/j.phytochem.2011.01.039;
RA   Weitzel C., Petersen M.;
RT   "Cloning and characterisation of rosmarinic acid synthase from Melissa
RT   officinalis L.";
RL   Phytochemistry 72:572-578(2011).
CC   -!- FUNCTION: Catalyzes the formation of rosmarinic acid using caffeoyl-CoA
CC       and 3.4-dihydroxyphenyllactate as substrates. Can also use 4-coumaroyl-
CC       CoA and 4-hydroxyphenyllactate as substrates. Rosmarinic acid is an
CC       ester of caffeic acid and 3,4-dihydroxyphenyllactic acid.
CC       {ECO:0000269|PubMed:21354582}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=(2R)-3-(3,4-dihydroxyphenyl)lactate + (E)-caffeoyl-CoA = (R)-
CC         rosmarinate + CoA; Xref=Rhea:RHEA:22344, ChEBI:CHEBI:57287,
CC         ChEBI:CHEBI:71492, ChEBI:CHEBI:71493, ChEBI:CHEBI:87136;
CC         EC=2.3.1.140; Evidence={ECO:0000269|PubMed:21354582};
CC   -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC       Kinetic parameters:
CC         KM=4.8 uM for 4-coumaroyl-CoA (with 4-hydroxyphenyllactate as
CC         acceptor substrate) {ECO:0000269|PubMed:21354582};
CC         KM=5.8 uM for caffeoyl-CoA (with 4-hydroxyphenyllactate as acceptor
CC         substrate) {ECO:0000269|PubMed:21354582};
CC         KM=7.3 uM for 4-coumaroyl-CoA (with 3,4-dihydroxyphenyllactic acid as
CC         acceptor substrate) {ECO:0000269|PubMed:21354582};
CC         KM=5.2 uM for caffeoyl-CoA (with 3,4-dihydroxyphenyllactic acid as
CC         acceptor substrate) {ECO:0000269|PubMed:21354582};
CC       pH dependence:
CC         Optimum pH is 7.1. {ECO:0000269|PubMed:21354582};
CC       Temperature dependence:
CC         Optimum temperature is 45 degrees Celsius.
CC         {ECO:0000269|PubMed:21354582};
CC   -!- SIMILARITY: Belongs to the plant acyltransferase family. {ECO:0000305}.
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DR   EMBL; FR670523; CBW35684.1; -; Genomic_DNA.
DR   AlphaFoldDB; G0LD36; -.
DR   SMR; G0LD36; -.
DR   PRIDE; G0LD36; -.
DR   KEGG; ag:CBW35684; -.
DR   GO; GO:0050266; F:rosmarinate synthase activity; IDA:UniProtKB.
DR   Gene3D; 3.30.559.10; -; 2.
DR   InterPro; IPR023213; CAT-like_dom_sf.
PE   1: Evidence at protein level;
KW   Acyltransferase; Transferase.
FT   CHAIN           1..427
FT                   /note="Rosmarinate synthase"
FT                   /id="PRO_0000422583"
FT   REGION          181..208
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   ACT_SITE        152
FT                   /note="Proton acceptor"
FT                   /evidence="ECO:0000255"
FT   ACT_SITE        374
FT                   /note="Proton acceptor"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   427 AA;  47161 MW;  4B4383045C3AB9E2 CRC64;
     MRIDIKDSTM VKPAAETPGG SVWLTNLDLL SPANYHTLSV HFYHHDGSEN FFDAAALKEA
     LSRALVDFYP YAGRLKLKDN RLEIDCNGEG VLLVEAESDG ALAELGEFAP RPDLNLIPQV
     DYAKGISTYP LMLFQLTRFK CGGVGLGVAN EHHLSDGVAA LHFINTWAHL ARGVPAPSPP
     PVFDRRSLSA RNPPKPQFSH AEYQPPPTLP TPLTDTAIAY SKLKVTRDQL GALKAKCLAG
     DPSGKPRSTF EVLAGHIWRC VCAARGLPED QETKLHIPFD GRAKLRLPPG YFGNAIFFAT
     PVATCGEIES NSLAHAVKRV GDAIARLDED YLRSSIDFLE LQEDISKLAQ GAHSFRCPNL
     WVISWVRLPV YEPDFGWGKA VYMGPWAAPF EGKSYLLPNP DNDGSLFVAI TLHTQHMERF
     EKLFYEI