RATB_ECOLI
ID RATB_ECOLI Reviewed; 96 AA.
AC P52119; P76603;
DT 01-OCT-1996, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-1996, sequence version 1.
DT 03-AUG-2022, entry version 131.
DE RecName: Full=UPF0125 protein RatB;
GN Name=ratB; Synonyms=pasI, yfjF; OrderedLocusNames=b2618, JW2599;
OS Escherichia coli (strain K12).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=83333;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=9205837; DOI=10.1093/dnares/4.2.91;
RA Yamamoto Y., Aiba H., Baba T., Hayashi K., Inada T., Isono K., Itoh T.,
RA Kimura S., Kitagawa M., Makino K., Miki T., Mitsuhashi N., Mizobuchi K.,
RA Mori H., Nakade S., Nakamura Y., Nashimoto H., Oshima T., Oyama S.,
RA Saito N., Sampei G., Satoh Y., Sivasundaram S., Tagami H., Takahashi H.,
RA Takeda J., Takemoto K., Uehara K., Wada C., Yamagata S., Horiuchi T.;
RT "Construction of a contiguous 874-kb sequence of the Escherichia coli-K12
RT genome corresponding to 50.0-68.8 min on the linkage map and analysis of
RT its sequence features.";
RL DNA Res. 4:91-113(1997).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA Shao Y.;
RT "The complete genome sequence of Escherichia coli K-12.";
RL Science 277:1453-1462(1997).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=16738553; DOI=10.1038/msb4100049;
RA Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT and W3110.";
RL Mol. Syst. Biol. 2:E1-E5(2006).
RN [4]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-20.
RC STRAIN=K12;
RX PubMed=7524073; DOI=10.1073/pnas.91.20.9223;
RA Komine Y., Kitabatake M., Yokogawa T., Nishikawa K., Inokuchi H.;
RT "A tRNA-like structure is present in 10Sa RNA, a small stable RNA from
RT Escherichia coli.";
RL Proc. Natl. Acad. Sci. U.S.A. 91:9223-9227(1994).
RN [5]
RP IDENTIFICATION BY MASS SPECTROMETRY.
RC STRAIN=B / BL21;
RX PubMed=10493123;
RX DOI=10.1002/(sici)1522-2683(19990801)20:11<2181::aid-elps2181>3.0.co;2-q;
RA Fountoulakis M., Takacs M.-F., Berndt P., Langen H., Takacs B.;
RT "Enrichment of low abundance proteins of Escherichia coli by hydroxyapatite
RT chromatography.";
RL Electrophoresis 20:2181-2195(1999).
RN [6]
RP LACK OF ANTITOXIN FUNCTION.
RX PubMed=21323758; DOI=10.1111/j.1365-2958.2010.07506.x;
RA Zhang Y., Inouye M.;
RT "RatA (YfjG), an Escherichia coli toxin, inhibits 70S ribosome association
RT to block translation initiation.";
RL Mol. Microbiol. 79:1418-1429(2011).
RN [7]
RP DISRUPTION PHENOTYPE.
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=23055930; DOI=10.1371/journal.ppat.1002954;
RA Norton J.P., Mulvey M.A.;
RT "Toxin-antitoxin systems are important for niche-specific colonization and
RT stress resistance of uropathogenic Escherichia coli.";
RL PLoS Pathog. 8:E1002954-E1002954(2012).
CC -!- FUNCTION: Does not function as an antitoxin to the upstream RatA toxin.
CC -!- DISRUPTION PHENOTYPE: Deletion of the ratA-ratB operon has no effect on
CC bacterial persistence on rich medium for this strain, but does affect
CC bacterial persistence for E.coli strain CFT073.
CC {ECO:0000269|PubMed:23055930}.
CC -!- SIMILARITY: Belongs to the UPF0125 (RnfH) family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAA79788.1; Type=Erroneous initiation; Note=Extended N-terminus.; Evidence={ECO:0000305};
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DR EMBL; U36840; AAA79788.1; ALT_INIT; Genomic_DNA.
DR EMBL; U00096; AAC75667.2; -; Genomic_DNA.
DR EMBL; AP009048; BAA16503.1; -; Genomic_DNA.
DR EMBL; D12501; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR PIR; T08631; T08631.
DR RefSeq; NP_417108.4; NC_000913.3.
DR RefSeq; WP_001117838.1; NZ_STEB01000040.1.
DR AlphaFoldDB; P52119; -.
DR SMR; P52119; -.
DR BioGRID; 4263354; 33.
DR IntAct; P52119; 19.
DR STRING; 511145.b2618; -.
DR jPOST; P52119; -.
DR PaxDb; P52119; -.
DR PRIDE; P52119; -.
DR EnsemblBacteria; AAC75667; AAC75667; b2618.
DR EnsemblBacteria; BAA16503; BAA16503; BAA16503.
DR GeneID; 66673494; -.
DR GeneID; 945078; -.
DR KEGG; ecj:JW2599; -.
DR KEGG; eco:b2618; -.
DR PATRIC; fig|511145.12.peg.2716; -.
DR EchoBASE; EB2984; -.
DR eggNOG; COG2914; Bacteria.
DR HOGENOM; CLU_150721_1_0_6; -.
DR InParanoid; P52119; -.
DR OMA; DMIHVEV; -.
DR PhylomeDB; P52119; -.
DR BioCyc; EcoCyc:G7357-MON; -.
DR PRO; PR:P52119; -.
DR Proteomes; UP000000318; Chromosome.
DR Proteomes; UP000000625; Chromosome.
DR Gene3D; 3.10.20.280; -; 1.
DR HAMAP; MF_00460; UPF0125_RnfH; 1.
DR InterPro; IPR016155; Mopterin_synth/thiamin_S_b.
DR InterPro; IPR005346; RnfH.
DR InterPro; IPR037021; RnfH_sf.
DR PANTHER; PTHR37483; PTHR37483; 1.
DR Pfam; PF03658; Ub-RnfH; 1.
DR SUPFAM; SSF54285; SSF54285; 1.
PE 1: Evidence at protein level;
KW Reference proteome.
FT CHAIN 1..96
FT /note="UPF0125 protein RatB"
FT /id="PRO_0000192487"
SQ SEQUENCE 96 AA; 10789 MW; 87E099B00E238F18 CRC64;
MPGKIAVEVA YALPEKQYLQ RVTLQEGATV EEAIRASGLL ELRTDIDLTK NKVGIYSRPA
KLSDSVHDGD RVEIYRPLIA DPKELRRQRA EKSANK
