RAV1_SCHPO
ID RAV1_SCHPO Reviewed; 1297 AA.
AC Q9Y817;
DT 13-NOV-2007, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1999, sequence version 1.
DT 25-MAY-2022, entry version 120.
DE RecName: Full=Regulator of V-ATPase in vacuolar membrane protein 1;
DE AltName: Full=RAVE complex subunit rav1;
GN Name=rav1; ORFNames=SPBC1105.10;
OS Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Taphrinomycotina;
OC Schizosaccharomycetes; Schizosaccharomycetales; Schizosaccharomycetaceae;
OC Schizosaccharomyces.
OX NCBI_TaxID=284812;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=972 / ATCC 24843;
RX PubMed=11859360; DOI=10.1038/nature724;
RA Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A.,
RA Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S.,
RA Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.,
RA Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S.,
RA Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S.,
RA Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D.,
RA Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P.,
RA Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K.,
RA O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M.,
RA Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N.,
RA Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A.,
RA Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R.,
RA Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M.,
RA Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A.,
RA Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A.,
RA Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H.,
RA Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S.,
RA Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C.,
RA Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A.,
RA Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M.,
RA del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S.,
RA Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R.,
RA Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G.,
RA Nurse P.;
RT "The genome sequence of Schizosaccharomyces pombe.";
RL Nature 415:871-880(2002).
RN [2]
RP SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
RX PubMed=16823372; DOI=10.1038/nbt1222;
RA Matsuyama A., Arai R., Yashiroda Y., Shirai A., Kamata A., Sekido S.,
RA Kobayashi Y., Hashimoto A., Hamamoto M., Hiraoka Y., Horinouchi S.,
RA Yoshida M.;
RT "ORFeome cloning and global analysis of protein localization in the fission
RT yeast Schizosaccharomyces pombe.";
RL Nat. Biotechnol. 24:841-847(2006).
RN [3]
RP IDENTIFICATION IN THE RAVE COMPLEX, INTERACTION WITH VAM2, SUBCELLULAR
RP LOCATION, DISRUPTION PHENOTYPE, AND FUNCTION.
RX PubMed=18441123; DOI=10.1128/ec.00037-08;
RA Dawson K., Toone W.M., Jones N., Wilkinson C.R.;
RT "Loss of regulators of vacuolar ATPase function and ceramide synthesis
RT results in multidrug sensitivity in Schizosaccharomyces pombe.";
RL Eukaryot. Cell 7:926-937(2008).
CC -!- FUNCTION: Component of the RAVE complex which is required for stable
CC assembly of the vacuolar ATPase complex V-ATPase.
CC {ECO:0000269|PubMed:18441123}.
CC -!- SUBUNIT: Component of the RAVE complex composed of rav1, rav2 and skp1.
CC Interacts with vam2. {ECO:0000269|PubMed:18441123}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm. Nucleus. Endomembrane system
CC {ECO:0000250}.
CC -!- DISRUPTION PHENOTYPE: Leads to sensitivity to a range of drugs
CC including doxorubicin, camptothecin, cycloheximide, and bleomycine,
CC hydroxyurea, and fluconazole. {ECO:0000269|PubMed:18441123}.
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DR EMBL; CU329671; CAB50973.1; -; Genomic_DNA.
DR PIR; T39287; T39287.
DR RefSeq; NP_596466.1; NM_001022385.2.
DR AlphaFoldDB; Q9Y817; -.
DR BioGRID; 276258; 27.
DR STRING; 4896.SPBC1105.10.1; -.
DR iPTMnet; Q9Y817; -.
DR MaxQB; Q9Y817; -.
DR PaxDb; Q9Y817; -.
DR PRIDE; Q9Y817; -.
DR EnsemblFungi; SPBC1105.10.1; SPBC1105.10.1:pep; SPBC1105.10.
DR GeneID; 2539705; -.
DR KEGG; spo:SPBC1105.10; -.
DR PomBase; SPBC1105.10; rav1.
DR VEuPathDB; FungiDB:SPBC1105.10; -.
DR eggNOG; KOG1064; Eukaryota.
DR HOGENOM; CLU_000310_0_1_1; -.
DR InParanoid; Q9Y817; -.
DR OMA; TIECVAT; -.
DR PhylomeDB; Q9Y817; -.
DR PRO; PR:Q9Y817; -.
DR Proteomes; UP000002485; Chromosome II.
DR GO; GO:0005737; C:cytoplasm; IDA:PomBase.
DR GO; GO:0005829; C:cytosol; HDA:PomBase.
DR GO; GO:0010008; C:endosome membrane; IDA:PomBase.
DR GO; GO:0005634; C:nucleus; HDA:PomBase.
DR GO; GO:0043291; C:RAVE complex; IPI:PomBase.
DR GO; GO:0044877; F:protein-containing complex binding; IPI:PomBase.
DR GO; GO:0045324; P:late endosome to vacuole transport; TAS:PomBase.
DR GO; GO:0007035; P:vacuolar acidification; IBA:GO_Central.
DR GO; GO:0070072; P:vacuolar proton-transporting V-type ATPase complex assembly; TAS:PomBase.
DR InterPro; IPR022033; Rav1p_C.
DR InterPro; IPR036322; WD40_repeat_dom_sf.
DR Pfam; PF12234; Rav1p_C; 1.
DR SUPFAM; SSF50978; SSF50978; 1.
PE 1: Evidence at protein level;
KW Cytoplasm; Membrane; Nucleus; Reference proteome.
FT CHAIN 1..1297
FT /note="Regulator of V-ATPase in vacuolar membrane protein
FT 1"
FT /id="PRO_0000310740"
FT REGION 1161..1182
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1260..1297
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1161..1175
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 1297 AA; 148739 MW; ECD30BCFBB91D03C CRC64;
MPLAFTNPGT PLKRTAAYDN VQWNQNRILT FGTRYTVLII VNNYESYQNI QFGESIGAVA
LDQRTGFLAI AHGQVLDIYK PADSLKWTHH YNYITQLSED ITQLSWGYNF DLLLCASSYT
KLINFSEDKP RLVWSQNRIS DSFLSSTISS DACFIASIET KNPLAKVWQR TSPQGTVSSC
DDFSYLVHPC RVRFSQWSKH LHPDASGSTP FLSTVGYDNV LRIWQVGSRF GSQLMHLSCF
LNLRKYYKSD QPPYCCFIDK DDFTIALAHA ISRHSGQVHK DRILNRLLAL ASGQPHIFLL
FTFECLYIFS LVIDQETLSS FELINQVPTN FPKGALQPDG FIPIYRTSKH EFYDYNIILF
FQNSAREYMF SLTDFFYSDF ELRVSYDFYG HDYPIKSLTR TSNGHGIVSR DVNRICMFQS
YISQDGKNQL ICRFRLLLGE SDFILPLYAG EYAAVLNLNS LKLWHCNEAI PPTLLCTCRQ
VPTSPIITFF ILPVQNNDNS ALCALTEGGH AWFWSVSNEA HDGSAVLRFI DRVNFTKNLN
IASAVDVMGW SSTLNLSSLS SFDQEVFQSI SKDGLLQTWM ARVLGDDKAE ISEISKVQTS
IKSATMIKGS TSKKVAVVSE NNRRLSIFDT RSSEFSEKEE FSSVFDDYGP INDLDWTSTP
NSHSILAVGF LHDVILLCQN RRSYMNDIPC WSRIRRYNLL EYTNSLISDS SWLDNGTLVT
AIGNGLFYFD NSLPEDQSLL FPASLKRSAK NIFDLAYQLN GILPVFHPQL LQQLLLRNQP
FLFTNILLRF YLCLKDDVDM HFLLNMDCSE FYCCDEEISK DLLIKSLSQN SAEILNPAEL
DFEETSVSKH LGLYIDEMIK KLTALLKVKK VKTLTRSSQF LLLNLIEAFN KARALRDSLD
LNGKRYCIML NQYVLSKHQR QLTSLPTREM AWAFHSSNKA VLLDHTKKLH GKPLLWNAVE
EYAIPFWLNE QLLKDVFLEL SRNHYAEIDD RNPENVSLYH IALHKINVFR DLWRLASWHK
ESARTVKLLS NDFSKKKWKV VASKNAFALL SKHRYFYASA FFLLADSCYD AAKVCIRNLK
SISLAIAMTR VYEGDDGPTL KRLINEYVLP LAVSQNDRWL TNWSFTILKQ EKKALQSLVS
PIRSLVSDND FIQLYTSDKN DQEIHENKDS QRNSTNEPVR NKFPSDDPAF ILLYECLRSS
NVQIDESLEY RFVLHNANVY CQLGCDLIAL GLLRNWKFQK NPATAHLETE DYMVTDKQSA
VEKTHDSPKL GKTLMGQDIR PTPDDVPEFN ESAFSFE
