RAV2_YEAST
ID RAV2_YEAST Reviewed; 351 AA.
AC Q03956; D6VSI4;
DT 01-FEB-2005, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1996, sequence version 1.
DT 03-AUG-2022, entry version 140.
DE RecName: Full=Regulator of V-ATPase in vacuolar membrane protein 2;
GN Name=RAV2; OrderedLocusNames=YDR202C; ORFNames=YD9346.12C;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=9169867;
RA Jacq C., Alt-Moerbe J., Andre B., Arnold W., Bahr A., Ballesta J.P.G.,
RA Bargues M., Baron L., Becker A., Biteau N., Bloecker H., Blugeon C.,
RA Boskovic J., Brandt P., Brueckner M., Buitrago M.J., Coster F.,
RA Delaveau T., del Rey F., Dujon B., Eide L.G., Garcia-Cantalejo J.M.,
RA Goffeau A., Gomez-Peris A., Granotier C., Hanemann V., Hankeln T.,
RA Hoheisel J.D., Jaeger W., Jimenez A., Jonniaux J.-L., Kraemer C.,
RA Kuester H., Laamanen P., Legros Y., Louis E.J., Moeller-Rieker S.,
RA Monnet A., Moro M., Mueller-Auer S., Nussbaumer B., Paricio N., Paulin L.,
RA Perea J., Perez-Alonso M., Perez-Ortin J.E., Pohl T.M., Prydz H.,
RA Purnelle B., Rasmussen S.W., Remacha M.A., Revuelta J.L., Rieger M.,
RA Salom D., Saluz H.P., Saiz J.E., Saren A.-M., Schaefer M., Scharfe M.,
RA Schmidt E.R., Schneider C., Scholler P., Schwarz S., Soler-Mira A.,
RA Urrestarazu L.A., Verhasselt P., Vissers S., Voet M., Volckaert G.,
RA Wagner G., Wambutt R., Wedler E., Wedler H., Woelfl S., Harris D.E.,
RA Bowman S., Brown D., Churcher C.M., Connor R., Dedman K., Gentles S.,
RA Hamlin N., Hunt S., Jones L., McDonald S., Murphy L.D., Niblett D.,
RA Odell C., Oliver K., Rajandream M.A., Richards C., Shore L., Walsh S.V.,
RA Barrell B.G., Dietrich F.S., Mulligan J.T., Allen E., Araujo R., Aviles E.,
RA Berno A., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M.,
RA Hunicke-Smith S., Hyman R.W., Komp C., Lashkari D., Lew H., Lin D.,
RA Mosedale D., Nakahara K., Namath A., Oefner P., Oh C., Petel F.X.,
RA Roberts D., Schramm S., Schroeder M., Shogren T., Shroff N., Winant A.,
RA Yelton M.A., Botstein D., Davis R.W., Johnston M., Andrews S., Brinkman R.,
RA Cooper J., Ding H., Du Z., Favello A., Fulton L., Gattung S., Greco T.,
RA Hallsworth K., Hawkins J., Hillier L.W., Jier M., Johnson D., Johnston L.,
RA Kirsten J., Kucaba T., Langston Y., Latreille P., Le T., Mardis E.,
RA Menezes S., Miller N., Nhan M., Pauley A., Peluso D., Rifkin L., Riles L.,
RA Taich A., Trevaskis E., Vignati D., Wilcox L., Wohldman P., Vaudin M.,
RA Wilson R., Waterston R., Albermann K., Hani J., Heumann K., Kleine K.,
RA Mewes H.-W., Zollner A., Zaccaria P.;
RT "The nucleotide sequence of Saccharomyces cerevisiae chromosome IV.";
RL Nature 387:75-78(1997).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=17322287; DOI=10.1101/gr.6037607;
RA Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F.,
RA Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J.,
RA Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J.,
RA Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D.,
RA LaBaer J.;
RT "Approaching a complete repository of sequence-verified protein-encoding
RT clones for Saccharomyces cerevisiae.";
RL Genome Res. 17:536-543(2007).
RN [4]
RP FUNCTION, IDENTIFICATION IN THE RAVE COMPLEX WITH RAV1 AND CBF3D, AND
RP INTERACTION WITH RAV1; CBF3D; VMA1; VMA2; VMA4 AND VMA8.
RX PubMed=11283612; DOI=10.1038/35070067;
RA Seol J.H., Shevchenko A., Shevchenko A., Deshaies R.J.;
RT "Skp1 forms multiple protein complexes, including RAVE, a regulator of V-
RT ATPase assembly.";
RL Nat. Cell Biol. 3:384-391(2001).
RN [5]
RP FUNCTION OF THE RAVE COMPLEX.
RX PubMed=11844802; DOI=10.1074/jbc.m200682200;
RA Smardon A.M., Tarsio M., Kane P.M.;
RT "The RAVE complex is essential for stable assembly of the yeast V-ATPase.";
RL J. Biol. Chem. 277:13831-13839(2002).
RN [6]
RP LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
RX PubMed=14562106; DOI=10.1038/nature02046;
RA Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N.,
RA O'Shea E.K., Weissman J.S.;
RT "Global analysis of protein expression in yeast.";
RL Nature 425:737-741(2003).
CC -!- FUNCTION: Component of the RAVE complex, which is required for stable
CC assembly of the vacuolar ATPase complex V-ATPase under many conditions.
CC May be required for transport between the early endosome and the late
CC endosome/prevacuolar compartment (PVC). {ECO:0000269|PubMed:11283612,
CC ECO:0000269|PubMed:11844802}.
CC -!- SUBUNIT: Component of the RAVE complex composed of RAV1, RAV2 and
CC CBF3D/SKP1. Within the complex, it interacts directly with RAV1 and
CC CBF3D. Interacts with the V-ATPase V1 subunits VMA1, VMA2 and VMA8.
CC {ECO:0000269|PubMed:11283612}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000305}. Early endosome membrane
CC {ECO:0000305}; Peripheral membrane protein {ECO:0000305}; Cytoplasmic
CC side {ECO:0000305}. Note=May be membrane-associated and localize to
CC early endosomes via its interaction with RAV1.
CC -!- MISCELLANEOUS: Present with 2860 molecules/cell in log phase SD medium.
CC {ECO:0000269|PubMed:14562106}.
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DR EMBL; Z48784; CAA88714.1; -; Genomic_DNA.
DR EMBL; AY557695; AAS56021.1; -; Genomic_DNA.
DR EMBL; BK006938; DAA12044.1; -; Genomic_DNA.
DR PIR; S52708; S52708.
DR RefSeq; NP_010488.1; NM_001180510.1.
DR AlphaFoldDB; Q03956; -.
DR BioGRID; 32253; 186.
DR ComplexPortal; CPX-1627; RAVE complex.
DR DIP; DIP-8529N; -.
DR IntAct; Q03956; 11.
DR MINT; Q03956; -.
DR STRING; 4932.YDR202C; -.
DR iPTMnet; Q03956; -.
DR MaxQB; Q03956; -.
DR PaxDb; Q03956; -.
DR PRIDE; Q03956; -.
DR EnsemblFungi; YDR202C_mRNA; YDR202C; YDR202C.
DR GeneID; 851784; -.
DR KEGG; sce:YDR202C; -.
DR SGD; S000002610; RAV2.
DR VEuPathDB; FungiDB:YDR202C; -.
DR eggNOG; ENOG502RBKJ; Eukaryota.
DR HOGENOM; CLU_813985_0_0_1; -.
DR InParanoid; Q03956; -.
DR OMA; EISIDFR; -.
DR BioCyc; YEAST:G3O-29787-MON; -.
DR PRO; PR:Q03956; -.
DR Proteomes; UP000002311; Chromosome IV.
DR RNAct; Q03956; protein.
DR GO; GO:0031901; C:early endosome membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0012505; C:endomembrane system; IC:ComplexPortal.
DR GO; GO:0043291; C:RAVE complex; IPI:SGD.
DR GO; GO:0015031; P:protein transport; IEA:UniProtKB-KW.
DR GO; GO:0043254; P:regulation of protein-containing complex assembly; IMP:SGD.
DR GO; GO:0007035; P:vacuolar acidification; IMP:SGD.
DR GO; GO:0070072; P:vacuolar proton-transporting V-type ATPase complex assembly; IDA:ComplexPortal.
DR InterPro; IPR028241; RAVE2/Rogdi.
DR PANTHER; PTHR13618; PTHR13618; 1.
DR Pfam; PF10259; Rogdi_lz; 1.
PE 1: Evidence at protein level;
KW Cytoplasm; Endosome; Membrane; Protein transport; Reference proteome;
KW Transport.
FT CHAIN 1..351
FT /note="Regulator of V-ATPase in vacuolar membrane protein
FT 2"
FT /id="PRO_0000097175"
SQ SEQUENCE 351 AA; 40017 MW; 0194C41FBF3819E9 CRC64;
MSVDLFPNDR FGAEDKYDNF KDAVKECSWL IEEIVKPQLP NIIDNFSKCL EMLESDQIFK
MPVSNGIPNE SNKQNDSPTV KGVITRQGQY IVDFHIVVRF PQFQRGKQVM FRMNTGLNFL
LIQFSKIMTH LKNILEILNQ LQVATDVSEF VSKFGVAMEL LNHSLILLQN PPRDLVFPED
NNFAMKEMFQ DCYSVCESTA HILGLELTLC RNELCIELRN LIKVTKKPWC EIDSKTGRSF
CDQIRNQVTN ERNKTLSKIL SENGVQVQDS TLLNHIISSF QSEAITLPEA QELLRRGVTF
DNRVVMECEK LIVSTSDPTL ISISAKLNSL KASMANHQAN LVASKQLSTY K
