RAVA_ECOLI
ID RAVA_ECOLI Reviewed; 498 AA.
AC P31473; Q2M864;
DT 01-JUL-1993, integrated into UniProtKB/Swiss-Prot.
DT 15-JUL-1998, sequence version 2.
DT 03-AUG-2022, entry version 168.
DE RecName: Full=ATPase RavA;
DE EC=3.6.3.-;
DE AltName: Full=Regulatory ATPase variant A;
GN Name=ravA; Synonyms=yieN; OrderedLocusNames=b3746, JW3725;
OS Escherichia coli (strain K12).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=83333;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=7686882; DOI=10.1006/geno.1993.1230;
RA Burland V.D., Plunkett G. III, Daniels D.L., Blattner F.R.;
RT "DNA sequence and analysis of 136 kilobases of the Escherichia coli genome:
RT organizational symmetry around the origin of replication.";
RL Genomics 16:551-561(1993).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], AND SEQUENCE REVISION.
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA Shao Y.;
RT "The complete genome sequence of Escherichia coli K-12.";
RL Science 277:1453-1462(1997).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=16738553; DOI=10.1038/msb4100049;
RA Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT and W3110.";
RL Mol. Syst. Biol. 2:E1-E5(2006).
RN [4]
RP CHARACTERIZATION, SUBUNIT, AND INTERACTION WITH CADA AND VIAA.
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=16301313; DOI=10.1074/jbc.m511172200;
RA Snider J., Gutsche I., Lin M., Baby S., Cox B., Butland G., Greenblatt J.,
RA Emili A., Houry W.A.;
RT "Formation of a distinctive complex between the inducible bacterial lysine
RT decarboxylase and a novel AAA+ ATPase.";
RL J. Biol. Chem. 281:1532-1546(2006).
CC -!- FUNCTION: Functions as an ATPase. May play a role in metal insertion
CC (metal-chelatase) or as a chaperone.
CC -!- ACTIVITY REGULATION: The complex formed with CadA represents a possible
CC means of regulating RavA activity in response to acid stress
CC conditions. This interaction results in an increase in RavA ATPase
CC activity.
CC -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC Kinetic parameters:
CC KM=790 uM for ATP (at pH 7.5);
CC Note=RavA also has GTPase activity.;
CC pH dependence:
CC Optimum pH is 7.5.;
CC -!- SUBUNIT: Hexameric oligomer. Interactions of five RavA oligomers with
CC two CadA decamers. Possible formation of a ternary complex RavA-ViaA-
CC CadA. {ECO:0000269|PubMed:16301313}.
CC -!- INTERACTION:
CC P31473; P0A9H3: cadA; NbExp=13; IntAct=EBI-561223, EBI-545922;
CC P31473; P31473: ravA; NbExp=7; IntAct=EBI-561223, EBI-561223;
CC -!- SUBCELLULAR LOCATION: Cytoplasm.
CC -!- INDUCTION: Expression is sigma S-dependent.
CC -!- SIMILARITY: Belongs to the RavA family. {ECO:0000305}.
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DR EMBL; L10328; AAA62099.1; ALT_FRAME; Genomic_DNA.
DR EMBL; U00096; AAC76769.2; -; Genomic_DNA.
DR EMBL; AP009048; BAE77542.1; -; Genomic_DNA.
DR PIR; C65178; C65178.
DR RefSeq; NP_418202.4; NC_000913.3.
DR RefSeq; WP_001299914.1; NZ_LN832404.1.
DR PDB; 3NBX; X-ray; 2.91 A; X=1-498.
DR PDB; 4UPB; EM; 11.00 A; C/D/E=1-498.
DR PDB; 4UPF; EM; 7.50 A; D=329-440.
DR PDB; 5FL2; EM; 6.20 A; K=332-437.
DR PDB; 6Q7L; EM; 7.60 A; U=1-498.
DR PDB; 6SZA; EM; 6.00 A; A/B/C/D/E/F=1-498.
DR PDB; 6SZB; EM; 7.00 A; A/B/C/D/E/F=1-498.
DR PDBsum; 3NBX; -.
DR PDBsum; 4UPB; -.
DR PDBsum; 4UPF; -.
DR PDBsum; 5FL2; -.
DR PDBsum; 6Q7L; -.
DR PDBsum; 6SZA; -.
DR PDBsum; 6SZB; -.
DR AlphaFoldDB; P31473; -.
DR SMR; P31473; -.
DR BioGRID; 4262601; 626.
DR DIP; DIP-12469N; -.
DR IntAct; P31473; 22.
DR STRING; 511145.b3746; -.
DR jPOST; P31473; -.
DR PaxDb; P31473; -.
DR PRIDE; P31473; -.
DR EnsemblBacteria; AAC76769; AAC76769; b3746.
DR EnsemblBacteria; BAE77542; BAE77542; BAE77542.
DR GeneID; 948256; -.
DR KEGG; ecj:JW3725; -.
DR KEGG; eco:b3746; -.
DR PATRIC; fig|511145.12.peg.3871; -.
DR EchoBASE; EB1682; -.
DR eggNOG; COG0714; Bacteria.
DR HOGENOM; CLU_018678_1_0_6; -.
DR InParanoid; P31473; -.
DR OMA; HANAFEY; -.
DR PhylomeDB; P31473; -.
DR BioCyc; EcoCyc:EG11731-MON; -.
DR BioCyc; MetaCyc:EG11731-MON; -.
DR EvolutionaryTrace; P31473; -.
DR PRO; PR:P31473; -.
DR Proteomes; UP000000318; Chromosome.
DR Proteomes; UP000000625; Chromosome.
DR GO; GO:0005737; C:cytoplasm; IDA:EcoCyc.
DR GO; GO:0005829; C:cytosol; IDA:EcoCyc.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0016887; F:ATP hydrolysis activity; IDA:EcoCyc.
DR GO; GO:0042802; F:identical protein binding; IPI:IntAct.
DR Gene3D; 3.40.50.300; -; 1.
DR HAMAP; MF_01625; ATPase_RavA; 1.
DR InterPro; IPR003593; AAA+_ATPase.
DR InterPro; IPR023671; ATPase_RavA.
DR InterPro; IPR022547; ATPase_RavA_C.
DR InterPro; IPR045427; MoxR.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR041538; RavA-like_AAA_lid.
DR Pfam; PF17868; AAA_lid_8; 1.
DR Pfam; PF20030; bpMoxR; 1.
DR Pfam; PF12592; DUF3763; 1.
DR SMART; SM00382; AAA; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
PE 1: Evidence at protein level;
KW 3D-structure; ATP-binding; Cytoplasm; Hydrolase; Nucleotide-binding;
KW Reference proteome.
FT CHAIN 1..498
FT /note="ATPase RavA"
FT /id="PRO_0000209370"
FT HELIX 4..19
FT /evidence="ECO:0007829|PDB:3NBX"
FT HELIX 26..38
FT /evidence="ECO:0007829|PDB:3NBX"
FT STRAND 41..45
FT /evidence="ECO:0007829|PDB:3NBX"
FT STRAND 48..51
FT /evidence="ECO:0007829|PDB:3NBX"
FT HELIX 52..58
FT /evidence="ECO:0007829|PDB:3NBX"
FT HELIX 59..62
FT /evidence="ECO:0007829|PDB:3NBX"
FT STRAND 63..65
FT /evidence="ECO:0007829|PDB:3NBX"
FT STRAND 68..72
FT /evidence="ECO:0007829|PDB:3NBX"
FT HELIX 79..83
FT /evidence="ECO:0007829|PDB:3NBX"
FT HELIX 105..107
FT /evidence="ECO:0007829|PDB:3NBX"
FT STRAND 109..115
FT /evidence="ECO:0007829|PDB:3NBX"
FT HELIX 116..118
FT /evidence="ECO:0007829|PDB:3NBX"
FT HELIX 121..132
FT /evidence="ECO:0007829|PDB:3NBX"
FT STRAND 133..137
FT /evidence="ECO:0007829|PDB:3NBX"
FT STRAND 139..144
FT /evidence="ECO:0007829|PDB:3NBX"
FT STRAND 149..156
FT /evidence="ECO:0007829|PDB:3NBX"
FT HELIX 165..168
FT /evidence="ECO:0007829|PDB:3NBX"
FT STRAND 173..176
FT /evidence="ECO:0007829|PDB:3NBX"
FT HELIX 183..190
FT /evidence="ECO:0007829|PDB:3NBX"
FT TURN 203..205
FT /evidence="ECO:0007829|PDB:3NBX"
FT HELIX 209..219
FT /evidence="ECO:0007829|PDB:3NBX"
FT HELIX 226..241
FT /evidence="ECO:0007829|PDB:3NBX"
FT STRAND 243..245
FT /evidence="ECO:0007829|PDB:3NBX"
FT HELIX 250..266
FT /evidence="ECO:0007829|PDB:3NBX"
FT HELIX 274..282
FT /evidence="ECO:0007829|PDB:3NBX"
FT HELIX 288..304
FT /evidence="ECO:0007829|PDB:3NBX"
FT TURN 305..307
FT /evidence="ECO:0007829|PDB:3NBX"
FT HELIX 309..329
FT /evidence="ECO:0007829|PDB:3NBX"
FT TURN 354..356
FT /evidence="ECO:0007829|PDB:3NBX"
FT STRAND 360..372
FT /evidence="ECO:0007829|PDB:3NBX"
FT STRAND 375..383
FT /evidence="ECO:0007829|PDB:3NBX"
FT HELIX 384..393
FT /evidence="ECO:0007829|PDB:3NBX"
FT STRAND 398..401
FT /evidence="ECO:0007829|PDB:3NBX"
FT STRAND 405..409
FT /evidence="ECO:0007829|PDB:3NBX"
FT STRAND 411..414
FT /evidence="ECO:0007829|PDB:3NBX"
FT STRAND 420..423
FT /evidence="ECO:0007829|PDB:3NBX"
FT STRAND 429..433
FT /evidence="ECO:0007829|PDB:3NBX"
FT HELIX 444..465
FT /evidence="ECO:0007829|PDB:3NBX"
FT HELIX 475..495
FT /evidence="ECO:0007829|PDB:3NBX"
SQ SEQUENCE 498 AA; 56389 MW; 3D2EDCAA17240BE9 CRC64;
MAHPHLLAER ISRLSSSLEK GLYERSHAIR LCLLAALSGE SVFLLGPPGI AKSLIARRLK
FAFQNARAFE YLMTRFSTPE EVFGPLSIQA LKDEGRYERL TSGYLPEAEI VFLDEIWKAG
PAILNTLLTA INERQFRNGA HVEKIPMRLL VAASNELPEA DSSLEALYDR MLIRLWLDKV
QDKANFRSML TSQQDENDNP VPDALQVTDE EYERWQKEIG EITLPDHVFE LIFMLRQQLD
KLPDAPYVSD RRWKKAIRLL QASAFFSGRS AVAPVDLILL KDCLWYDAQS LNLIQQQIDV
LMTGHAWQQQ GMLTRLGAIV QRHLQLQQQQ SDKTALTVIR LGGIFSRRQQ YQLPVNVTAS
TLTLLLQKPL KLHDMEVVHI SFERSALEQW LSKGGEIRGK LNGIGFAQKL NLEVDSAQHL
VVRDVSLQGS TLALPGSSAE GLPGEIKQQL EELESDWRKQ HALFSEQQKC LFIPGDWLGR
IEASLQDVGA QIRQAQQC
